Abstract

MOLINA-GAYOSSO, E.; ANDRADE-HOYOS, P.; GARCIA-ESPINOSA, R.  and  SOSA-HERNANDEZ, C. M.. Survivability of three species of Phytophthora and two of Pythium substrates preserved in short and long term. Rev. Mex. Cienc. Agríc [online]. 2016, vol.7, n.7, pp.1759-1764. ISSN 2007-0934.

The maintenance and viability of microorganisms preserved in the collections is a common problem in laboratory diagnosis of plant diseases and research institutions, and even in those where they include species of the genera Phytophthora and Pythium and in which cryopreservation is not an option. The oomycetes conservation in sterile water is an economic and reliable method. They have been reported retention periods up to 23 years for species of Phytophthora. In this work were evaluated the ability to survival and viability of Phytophthora and Pythium species kept in soil for periods of three to seven years in sterile water for periods of 6 to 21 years. Viability was confirmed by plating on medium cornmeal-agar culture medium supplemented with antibiotics. None of the oomycetes survived preserved in soil conservation during periods while in sterile water conservation remained viable Phytophthora species for up to 21 years and Pythium for 7 years.

Keywords : antibiotics; corn-agar; cryopreservation; microorganisms; oomicetos.

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