Abstract

DIAZ GARCIA, Luis Antonio. Sequencing of V3 and V6 hypervariable regions of microorganisms in a digester of nopal. Rev. Mex. Cienc. Agríc [online]. 2016, vol.7, n.3, pp.559-572. ISSN 2007-0934.

The development of clean, efficient and low cost power generation strategies is one of the most important current challenges for society. The methanogenesis, a set of metabolic processes carried out by archaea in the final stage, is used in biodigesters to generate methane gas. Control of microorganisms in a digester is decisive in the efficiency of methane production; it also lays the foundation for change, by metabolic engineering, methanogenic microorganisms and improve efficiency. In this paper we identified the microorganisms present in a sample from a digester of nopal. To identify as many microorganisms as possible a metagenomic analysis was performed to determine two hypervariable sequences of ribosomal genes 16 s. The primers used allowed only identify organisms domain of prokaryotes; archaea they not identified. The group of organisms was the most represented phylum Firmicutes, an important group of bacteria whose function is the degradation of green material for the production of carbon dioxide.

Keywords : gen 16 s; metagenomics; methane.

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