Abstract

RAYA-MONTANO, Yurixhi Atenea et al. In vitro propagation of Laelia halbingeriana. Rev. Mex. Cienc. Agríc [online]. 2011, vol.2, n.spe3, pp.539-553. ISSN 2007-0934.

Laelia halbingeriana (Orchidaceae) is an epiphytic plant, endemic from Oaxaca's glen, threatened by its habitat shrinking due to deforestation, land-use changes and, illegal plant extraction. A methodology was developed in the Graduated College in Agricultural Sciences in 2010, for its in vitro propagation as a conservation strategy using Murashige and Skoog medium (MS) supplemented with 6-benzyladenine (BA) (0, 2.22 and 4.43 µM L^-1) and 3-naphthaleneacetic acid (NAA) (0, 2.6, 5.2 and 10.4 µM L^-1). With these media, seedlings of 2.5 cm with three concentrations of sucrose were grown (58.43, 87.64 and 116.85 µM L^-1) and five mineral salts of MS medium (50, 75, 100, 125 and 150%). For rooting, the seedlings were grown on MS medium supplemented with five concentrations of NAA (0, 2.6, 7.8, 10.4 and 13 µM L^-1) and five 3-indole butyric acid (IBA) (0, 4.92, 7.38, 9.84 and 12.3 µML¹). A completely randomized design with factorial arrangement and ten repetitions was used. In the multiplication, BA (2.22 µM L^-1) increased the average number of leaves per plant (3.93), while the longest (1.11 cm) was achieved by adding NAA (5.2 µM L-¹). The sugar and mineral salts concentration did not influenced the in vitro organogenesis of L. halbingeriana. Rooting showed higher number of roots per plant (2.62), obtained by reducing 75% of mineral salts, the addition of 7.8 µM L^-1 of NAA increased the number of roots (2.86).

Keywords : Orchidaceae; mineral salts; phytoregulators.

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