Abstract

HENAO-ARDILA, A.; QUINTANILLA-CARVAJAL, M.X; KLOTZ-CEBERIO, B.F  and  SERNA-JIMENEZ, J.A. Evaluation of inhibition of the Candida intermedia due to the effect of supernatants from bioprotectors and their synergistic effect. Rev. Mex. Ing. Quím [online]. 2015, vol.14, n.2, pp.373-381. ISSN 1665-2738.

In this work, the inhibitory effect of the supernatants of three commercial bioprotectors (HOLDBAC YM-C® 100DCU, MicroGARD 200® y Lyofasr FPR2®) was evaluated sn order to establish the most effective bioprotector against the food spoilage yeast Candidal intermedia. Additional, the synergistic effect of the bioprotectors was studied through an experimental design of optimized mixture to determine the optimum mixing ratio that maximizes inhibition of Candida intermedia at a concentration of 10 exp 5 CFU/mL. The highest inhibition was obtained using the supernatants of MicroGARD 200®, reducing the microbial load in 3.2 logarithmic cycles, while HOLDBAC YM-C® and Lyofast FPR2® reduced the microbial load in 1.7 and 1.2 logarithmic cycles respectively. It was determined that there is a synergistic effect between the supernatants of the bioprotectors and that the optimum mixing of bioprotectors that maximized inhibition of Candida intermediate contained 23.7 % of HOLDBAC and 76.3 % of MicroGARD, reducing the microbial load in 3.4 logarithmic cycles.

Keywords : bioprotectors; supernatants; Candida intermedia; optimum mixing ratio; maximum inhibition.

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