Abstract

HERRERA GARCIA, Mauricio; RODRIGUEZ DORANTES, Angélica Ma.  and  GUERRERO ZUNIGA, L. Angélica. Evaluation of growing, hemoperoxidasas activity and phenanthrene removal of the cell culture of Fouquieria splendens and Fouquieria fasciculata. Polibotánica [online]. 2008, n.25, pp.101-119. ISSN 1405-2768.

Knowledge about the metabolic potential of plants to transform and degrade xenobiotics has been generated by analyzing responses of in vitro plant cell cultures to contaminants, and these reactions have been considered a useful tool to evaluate the phytotoxicity, metabolic rates and persistence of xenobiotics in plants. The present study evaluated phenanthrene removal by suspension cell cultures of two species of Fouquieriaceae, Fouquieria splendens and F. fasciculata, focusing on the effect of the presence of this contaminant on cell viability and the activity of an enzymatic system. The results in both cultures showed that there was no damaging effect by phenanthrene, between 40 to 90% of which was removed by the suspension cells. The cell cultures of F. fasciculata have more phenanthrene removal capacity compared to those of F. splendens at the different lengths of time of exposure to the contaminant. An inductive effect of hemoperoxidase activity was observed simultaneously with phenanthrene removal; therefore, it is possibly related to transformation of the contaminant.

Keywords : cell cultures; phenanthrene; hemoperoxidases.

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