Abstract

BETANCURT-OLVERA, Marcela et al. COMPARISON OF SIX DNA EXTRACTION METHODS IN MEXICAN HAWTHORN (Crataegus mexicana Moc. & Sessé). Rev. fitotec. mex [online]. 2018, vol.41, n.1, pp.75-79.  Epub Sep 21, 2020. ISSN 0187-7380.  https://doi.org/10.35196/rfm.2018.1.75-79.

The use of molecular marker techniques starts with a good DNA extracts. Six DNA extraction methods were tested to identify the most adequate method that produces pure and integral DNA: Dumolin, Doyle, Tsumura, Núñez, modified CTAB and the commercial kit Wizard Genomic DNA on fresh and lyophilized shoots and in dried leaves of Mexican hawthorn (Crataegus Mexicana Moc. & Sessé). The highest DNA quality was obtained with the Doyle and CTAB methods, while the highest yields were obtained with the Kit and the Doyle methods. Likewise, it was found that lyophilized plant material produced higher yield in all the protocols in both fresh shoots and dry leaves. In the verification by PCR, it was found that the ribosomal 16S gene could be easily amplified with the Doyle and modified CTAB protocols as well as with the commercial kit.

Keywords : Crataegus mexicana; DNA quality; DNA purification; PCR; Mexican hawthorn.

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