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For reversed-phase chromatography, the separation was evaluated initially using two columns (Atlantis dC18 and Zor-bax Eclipse XDB C18) and the mobile phases described in Experimental section. The Atlantis column has been manufactured for use with highly aqueous mobile phases and is recommended for polar compound retention, whereas the Zorbax one has a deactivated stationary phase that can work over a wide pH range (2 to 9) and, in accordance to the manufacturer's specifications, provide more symmetrical chromatographic peaks, particularly for basic compounds.