Intente links en:Similares en:
Blood samples were provided by Khyber Teaching Hospital, Peshawar and stored at -20 °C. 500 μg standard of each of the drug i.e. ceftriaxone and cefaclor, was added to 5 mL plasma and deproteinized by mixing with 15 mL of acetonitrile. The mixture was centrifuged at the rate of 3500 rpm for 5 minutes. The supernatant was removed and diluted to 50 mL with distilled water. The resulting plasma solution has a concentration of 10 μgmL-1 with respect to both drugs. Plasma samples in concentration range of 0.5 μgmL-1, 2.5 μgmL-1 and 5 μgmL-1 were prepared by dilution with mobile phase. All spiked plasma samples were filtered through 0.45 um membrane filter and injected to the chromatographic system under the optimum chromatographic condition already mentioned.