SciELO Mexico - www.scielo.org.mx

Links

All the weights were measured with Sartorius GMBH GÜTTINGEN Germany, digital single pan balance (maximum capacity 30.00000g±0.00001 g). The chromatographic system consisted of an Acme 9000 Series HPLC equipped with SP 930 isocratic pump and UV 730 D detector (Young Lin, Korea). All the separations were carried out on C₁₈ column (5μm, 4.6 mm Ø x 250 mm, Teknokroma S. Coop. C. Ltd. Barcelona, Spain) with a guard column containing with identical packing material to that of the analytical column and a rheodyne injector with 20 μL loop were used. Degassing of the mobile phase was carried out with a ks300 KUM SUNG ultrasonic (Korea) sonicator. The biological samples were centrifuged by using CL international clinical centrifuge (USA). A ternary mixture of acetonitrile, methanol and triethyl amine (TEA) buffer (pH 7) in ratio of 1:1:2 v/v was used as mobile phase. The mobile phase was filtered through 0.45 um membrane filter (Milli-pore), degassed by sonication for 30 min and delivered at rate of 0.9 mLmin^-1. All the sample solutions were also filtered through micropore filtration assembly (Millipore) before injection. A 20 μl sample was injected to the column by using a 100 μL syringe (Hamilton Co., Reno. Nevada USA) and the column effluents were monitored with UV detector at 240 nm. All measurements were performed at ambient temperature i.e. 25 °C with run time of 10 min.