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All reagents were of HPLC grade purity or high grade purity. Triethyl amine (TEA), glacial acetic acid, acetonitrile and methanol (BioM laboratories Cerritos, USA) were used in this work. Standard reference ceftriaxone and cefaclor were provided by Cirin Pharmaceutical (Pvt) Ltd., Hattar, Pakistan. Commercial formulations were purchased from local market.