SciELO - Scientific Electronic Library Online

vol.25 número2Carbono y nitrógeno del suelo en los sistemas bosque mesófilo de montaña, agroforestal y monocultivo de café índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados

  • Não possue artigos similaresSimilares em SciELO


Revista Chapingo serie ciencias forestales y del ambiente

versão On-line ISSN 2007-4018versão impressa ISSN 2007-3828


VILLANUEVA-ALONZO, Hernán de J. et al. Proteolytic activity of wild fruits of Bromelia karatas L. of Yucatán, Mexico. Rev. Chapingo ser. cienc. for. ambient [online]. 2019, vol.25, n.2, pp.157-168.  Epub 19-Fev-2021. ISSN 2007-4018.


Bromeliads are a source of proteases that have not been fully studied.


To evaluate the proteolytic activity of wild fruits of Bromelia karatas L. under different temperature, pH and NaCl conditions, and to estimate the thermal stability of their proteases.

Materials and methods:

The effect of pH (6, 7, 8, 9, 10 and 12), incubation temperatures (30, 40, 50, 60 and 70 °C) and NaCl concentrations (5, 10 and 20 %) on the proteolytic activity of the fruits was compared. The thermal stability of the proteases was assessed at 30, 50 and 70 °C for 240 minutes. The molecular weights and isoelectric points of the proteases were estimated by non-reducing two-dimensional zymography. The data were analyzed using a one-factor ANOVA and Tukey's test (α = 0.05).

Results and discussion:

The proteolytic activity of B. karatas fruit extract (8.59 U·mg-1) was greater than that of Ananas comosus (L.) Merr. variety comosus (3.42 U·mg-1). The activity was higher at pH 6 and 7 and in concentrations lower than 5 % NaCl. Proteases were stable at 30 and 50 °C for 210 minutes. The two-dimensional zymogram, under non-reducing conditions, showed at least 40 light zones with apparent molecular weights between 27.3 and 290 kDa, potentially representing proteases.


The proteases of B. karatas have potential for application in the food industry.

Palavras-chave : Bromeliaceae; proteases; karatasin; 2D zymography.

        · resumo em Espanhol     · texto em Inglês | Espanhol     · Inglês ( pdf ) | Espanhol ( pdf )