Resumen

ENCALADA-MENA, Lisandro Alberto et al. In vitro anthelmintic evaluation of curcumin against the eggs and larvae of three Haemonchus contortus isolates with different susceptibility to ivermectin. Rev. mex. de cienc. pecuarias [online]. 2024, vol.15, n.4, pp.811-832.  Epub 21-Mar-2025. ISSN 2448-6698.  https://doi.org/10.22319/rmcp.v15i4.6440.

Increasing gastrointestinal nematode resistance to conventional anthelmintics (AH) is a growing worldwide problem. Among various sources, the search for alternative AH has focused on plant secondary metabolites such as curcumin. The in vitro AH activity of curcumin (CUR) was evaluated against three isolates of the ruminant nematode Haemonchus contortus with different susceptibilities to conventional AH. Four in vitro tests were run: egg hatching inhibition (EHI), larval migration inhibition (LMI), larval exsheatment inhibition (LEI) and 72 h mortality of unsheathed L3. Curcumin (CUR) concentration range was 0 - 8.5 μg CUR/mL in the EHI, LMI and mortality tests. In the LEI test it was 0 - 17.3 μg CUR/mL. Concentration-response curves were generated using a log-logistic regression. Experimental design was completely random and results were analyzed with an ANOVA. Curcumin did not exhibit AH activity in the EHI, LMI and mortality tests, but had a significant AH effect in the LEI test. This effect was strongest against the FMVZ-UADY isolate (EC50= 1.9 μg/mL, 95%CI= 1.58-2.31), followed by the Paraíso isolate (EC50= 3.2 μg/mL, 95%CI = 2.69-3.81) and the CENID-SAI, INIFAP isolate (EC50= 7.0 μg/mL; 95%CI= 6.58-7.43). At the evaluated doses, curcumin had an AH effect against exsheatment of H. contortus L3, but no effect on egg hatching, L3 migration or mortality of exsheathed L3.

Palabras llave : Polyphenol; Anthelmintic; Larval exsheathed; Polymeric stabilizers.

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