Abstract

RUIZ-GONZALEZ, Miguel Ángel; SOLANO-VIDAL, Roney; VALADEZ-MOCTEZUMA, Ernestina  and  SERRATO-CRUZ, Miguel Ángel. Chemical compounds of Tagetes lucida essential oil and effects against Botrytis cinerea. Rev. Mex. Cienc. Agríc [online]. 2025, vol.16, n.3, e3613.  Epub Aug 12, 2025. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v16i3.3613.

The environment influences the chemical composition of plant essential oil and its biological effect. The purpose of the study was to describe the chemical profile of Tagetes lucida essential oil and to evaluate the in vitro biological effect against B. cinerea. The study was conducted in Texcoco, Mexico in October 2021. Essential oil was obtained from flowering plants by hydrodistillation; the identification of chemical compounds was carried out using the GC-MS technique. The in vitro bioassay employed the method of poisoned agar and mycelium of B. cinerea of three days of growth. Twelve treatments were evaluated: essential oil 0.1, 0.5, 1, and 2%, Tween 20 at 0.1, 0.3, 0.5, 0.8, 1 and 2%, commercial fungicide, and absolute control (only with sterile double-distilled water). Every 24 h, radial growth of the fungus was measured with a digital vernier and growth rate and inhibition of mycelial growth were estimated. Thirty-one chemical compounds were identified, (1S)-(-)-β-Pinene (36.4%), 1, 3, 5, 7-Cyclooctatetraene (12.7%), eucalyptol (10.6%), and o-Cymene (6.1%). The concentrations of 0.1, 0.5, 1 and 2% inhibited the mycelial growth and sporulation of B. cinerea. The commercial fungicide and the 2% concentration totally inhibited the growth of the fungus. Tween 20 also inhibited mycelial growth. The LC50 was 0.06% and the LC95 was 1.69%. The abundance of terpenes in the essential oil of T. lucida showed a fungicidal effect against B. cinerea. The surfactant had minor effects.

Keywords : Tagetes lucida; B. cinerea; essential oil..

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