Resumen

ESPINOSA-HUERTA, Elsa et al. Resistance to Colletotrichum lindemuthianum in transgenic common bean expressing an Arabidopsis thaliana defensin gene. Rev. Mex. Cienc. Agríc [online]. 2013, vol.4, n.7, pp.1027-1042. ISSN 2007-0934.

The defensin gene from Arabidopsis thaliana is an antimicrobial peptide that provides protection to a wide array of pathogens. Transgenic common bean (Phaseolus vulgaris L.) plants of cv. Flor de Mayo Anita constitutively expressing the defensin pdf 1.2 gene were generated by Agrobacterium-mediated transformation via hypocotyls direct organogenic regeneration. The pdf 1. 2 gene under the control of promoter CaMV-35 S was expressed in transformed common bean plants challenged with Colletotrichum lindemuthianum. The observed response was a significant reduction in the formation of lesions and spore mass proliferation in T0, T1 and T3 lines as compared to non-transformed plants. Twenty-seven T3 common bean transgenic plants from 5 independent lines expressing pdf 1.2 gene showed resistance to C. lindemuthianum races 448 and 1472 compared to non-transformed plants. Analysis ofthe level of pdf 1.2 gene expression by Q-PCR indicated that all plants considered resistant or tolerant had similar levels of transcriptional expression as opposed to susceptible plants, which did not have detectable levels of gene expression.

Palabras llave : Phaseolus vulgaris L.; Agrobacterium tumefaciens; antimicrobial peptide; transformation, pdf 1.2 gene; regeneration.

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