SciELO - Scientific Electronic Library Online

vol.12 número2Efecto de los procesos de secado y encurtido sobre la capacidad antioxidante de los fitoquímicos del chiltepín (Capsicum annuum L. var. glabriusculum)Uso de catalizadores en los procesos Fischer-Tropsch índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados




Links relacionados

  • Não possue artigos similaresSimilares em SciELO


Revista mexicana de ingeniería química

versão impressa ISSN 1665-2738


CASTRO, A.; GONZALEZ, I.; TZOMPANTZI, F.  e  VINIEGRA-GONZALEZ, G.. Influence of the type of support and immobilization on the activity and stability of laccase enzyme (Trametes versicolor). Rev. Mex. Ing. Quím [online]. 2013, vol.12, n.2, pp.241-255. ISSN 1665-2738.

This work presents a comparative study of the influence of the nature of the substrate and the immobilization mechanism on laccase enzyme activity and stability. The supports employed were: hydrotalcite-like particles (ZnAl2), amorphous silica crystals, and glassy carbon surface. Two immobilization mechanisms were applied: one physical, by adsorption, and the other chemical, with two versions of covalent bonding. In the first, using silanes and glutaraldehyde (GA), in the second, thiols were used as anchoring reagents. Hydrotalcite and silica supports were characterized before and after immobilizing the enzyme by X-ray diffraction analysis (XRD), while in the case of glassy carbon supports electrochemical characterization was performed. The catalytic properties Km and Kcat/Km of every enzymatic system were evaluated in a complementary fashion, as well as the free enzyme. The kinetic characterizations were done using ABTS (ammonium 2,2' azino-bis-(3 -ethylbenzothiazoline-6-sulfonate acid)), as a reagent typical of laccase in 0.1 M acetate buffer solution pH 3.7. Efficiency of the enzyme adsorbed on each support it was demonstrated that the method of immobilization is decisive in the catalysis. Greater efficiency was observed in the system that employed glassy carbon with either immobilization mechanism and these results are analogous with the tree enzyme.

Palavras-chave : laccase; immobilization; thiol; glassy carbon; hydrotalcite.

        · resumo em Espanhol     · texto em Inglês     · Inglês ( pdf )


Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons