Resumo

GARCIA-ROMO, Joel Said et al. Nitrosative and oxidative anti-stress, and anti-inflammatory potential of Litopenaeus stylirostris cytoprotective fraction. Biotecnia [online]. 2025, vol.27, e2425.  Epub 26-Maio-2025. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v27.2425.

Biological activities of a fraction from Litopenaeus stylirostris muscle, containing a bioactive novel indolocarbazole alkaloid derivative, eicosapentaenoic acid, and dioctyl phthalate, were determined. Reactive nitrogen species (RNS) scavenging activity was studied by means of the sodium nitroprusside chemical assay, as well as the inhibition of nitric oxide (NO) production and the reduction of reactive oxygen species (ROS) in the RAW 264.7 macrophage cell line, and the regulation of cytokine production in human peripheral blood mononuclear cells, after both cell models were stimulated with Escherichia coli lipopolysaccharide. In addition, an in-silico analysis was performed to determine the possible interaction of the fraction with proteins related to inflammation. Results showed that the fraction decreases RNS, NO, and the intracellular ROS levels; furthermore, it was found capable of up- and down-regulating anti- and pro-inflammatory cytokines, respectively, suggesting the presence of compounds that could be associated to decreases of nitrosative and oxidative stress, as well as inflammation processes; furthermore, this could be corroborated by predicting the interactions between EPA and selected inflammatory proteins (iNOS, NF-κB and TNF-α).

Palavras-chave : Reactive nitrogen species; reactive oxygen species; nitric oxide; cytokines.

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