Resumen

GIO-TRUJILLO, José Alberto et al. Behavior of Cucurbita pepo L. var. “Grey Zucchini”, in native arbuscular mycorrhizal fungi propagation in soils with different management. Biotecnia [online]. 2024, vol.26, e1972.  Epub 08-Nov-2024. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v26.1972.

The symbiosis between arbuscular mycorrhizal fungi (AMF) and plants represents an important alternative to increase yields in agricultural crops. The objective of the study was to evaluate the propagation of AMF isolated from soils with different management, and inoculated in Cucurbita pepo var. “Grey Zucchini.” A trap culture was established as a means of AMF propagation and a block design was used (five treatments x 15 repetitions). The native AMF came from three systems (T1 = secondary vegetation; T2 = multipurpose system; T3 = agricultural system). A positive (CP = GLUMIX®MICORRIZAS) and negative (CN = no inoculation) control were used. Mycorrhizal inoculation began in the seedbed, using Peat Moss-sterile soil (50:50 v/v). The substrate method with 12.5 g of native inoculant (T1 = 46 spores; T2 = 32 spores and T3 = 22 spores) was used as the inoculation method. In CP, 5 g of product = 100 spores were used. The results indicate that the greatest mycorrhizal propagation occurred in CP and T1, with a spore multiplication of 135.20 ± 26.24 and 74.67 ± 5.56 spores in 10 g of soil, and identifying a native consortium made up of AMF spores from the Glomaceae and Gigasporacea families. In conclusion, C. pepo represents a promising host for the trapping and multiplication of AMF.

Palabras llave : Mycorrhizal fungi; trap culture; spores; colonization; inoculation.

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