Resumen

SOSA-LARIOS, Tonantzin Citlali et al. Testosterone treatment positively regulates protein and mRNA levels of Mn-dependent superoxide dismutase enzyme in pancreatic islets of male rats (Rattus norvegicus). TIP [online]. 2023, vol.26, e618.  Epub 20-Ago-2024. ISSN 1405-888X.  https://doi.org/10.22201/fesz.23958723e.2023.618.

The androgenic hormone testosterone protects against pharmacological-induced damage to pancreatic islets in rodent models of diabetes. The increased production of reactive oxygen species damage the pancreatic islets through oxidative stress and apoptosis. The precise protective mechanism of testosterone has yet to be determined. The aim of this study was to evaluate the effect of post-gonadectomy testosterone substitution on the expression of two key antioxidant enzymes, Mn-dependent superoxide dismutase and catalase. The protein expression of the enzymes was examined by immunohistochemistry in male rats: intact, gonadectomized, and gonadectomized followed by testosterone substitution. mRNA expression was analyzed by reverse transcription-quantitative polymerase chain reaction on pancreatic islets cultured with testosterone, dihydrotestosterone (DHT) or the vehicle. Testosterone increased the protein level of MnSOD in pancreatic tissue and its mRNA expression in cultured pancreatic islets. DHT (a non-aromatizing androgen) had similar effects. For catalase, only the mRNA expression increased by testosterone treatment. Testosterone induced overexpression of MnSOD protein and mRNA in pancreatic islets through a mechanism unrelated to androgen aromatization, most likely involving androgen receptors, demonstrating the implications of testosterone in preventing damage to insulin-producing cells by oxidative stress.

Palabras llave : androgens; antioxidant enzymes; oxidative stress; pancreatic beta cells; Rattus norvegicus.

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