Abstract

AMBRIZ, Enrique et al. Effect of arbuscular mycorrhizal fungi on the survival and growth of Dalbergia congestiflora plants propagated in vitro and from seed under greenhouse conditions. Polibotánica [online]. 2026, n.61, pp.259-271.  Epub May 18, 2026. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.61.15.

Endangered tree species, such as Dalbergia congestiflora Pittier (Fabaceae), have a low quality of growth and development, which makes it difficult for them to survive in greenhouses and fields. To mitigate this, strategies such as fertilization and the addition of beneficial microorganisms are used, highlighting arbuscular mycorrhizal fungi (AMF). The objective of the present research was to determine the effect of the addition of AMF on the survival, growth and development of greenhouse-grown D. congestiflora plants, using two propagation methods (micropropagated and seed-propagated plants). From shoots multiplied in vitro from callus, micropropagated plantlets were obtained, successfully acclimatized and subsequently grown in greenhouse. The seed-propagated plants were developed from germination in Petri dishes with wet filter paper and then grown in greenhouse. In both cases, the plants were inoculated with a commercial AMF product, using three concentrations of spores/plant (80, 128 and 176) and a control treatment (0 spores) (n=6), evaluating growth parameters such as survival, plant height, stem diameter, number of leaflets and leaf area, during 180 days. Inoculation with spores had variable effects depending on the type of plant propagation, the survival of micropropagated and seed-obtained plants was higher (p<0.05) in inoculation treatments, compared to the control treatment. In micropropagated plants, inoculation did not affect stem height or diameter, but with inoculation of 128 and 176 spores, they resulted in a larger leaf area at 180 days (p<0.05). In seed-propagated plants, with the three inoculation concentrations, an increase in plant height and stem diameter was obtained at 180 days (p<0.05). In these plants, with 176 spores, the number of leaflets was also significantly increased (p<0.05), and with 80 and 128 spores, the largest leaf area was produced at 180 days (p<0.05). Overall, seed-propagated inoculated plants showed the highest values in all evaluated variables compared to micropropagated plants.

Keywords : Campincerán; plant quality; mycorrhizae; propagation.

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