Abstract

CABANAS-RODRIGUEZ, Marcela et al. Growth of Laelia autumnalis and Encyclia cordigera seedlings as a function of sucrose and activated charcoal concentration. Polibotánica [online]. 2026, n.61, pp.221-234.  Epub May 18, 2026. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.61.13.

In plant tissue culture, different components are used to improve plant growth; among them, the addition of different concentrations of sucrose and activated charcoal to the culture media has favored the growth of many species. Most orchids grow slowly and have low photosynthetic capacity; this also occurs in vitro, although to a lesser extent; therefore, it is necessary to investigate the environmental components that promote the growth of this plants. The aim of the research was to evaluate the effect of three concentrations of sucrose and three concentrations activated charcoal on the in vitro growth of plants of Laelia autumnalis and Encyclia cordigera. The macro and micronutrients of the medium Murashige and Skoog (1962) were used, supplemented whit 0.5 mg L-1 of thiamine HCI, 100 mg L-1 of myo-inositol and three concentration whit activated charcoal (0, 1 and 3 g L-1). A completely randomized, factorial arrangement of treatments was used for each species, whit 10 replications and 10 seedlings per replication for each of the 9 treatments. In L. autumnalis, 45 g of sucrose and 1 g of activated charcoal generated the highest results for seedling height (31.97 mm), leaf length (13.44 mm) and root length (8.63 cm), plant dry matter and root (6.57 and 2.65 mg, respectively). In E. cordigera, 45 g of sucrose and 3 g of activated charcoal promoted the highest height of the aerial part (6.06 mm), number (5.78) and leaf length (21.54 mm), number of roots (8.02), protocorm diameter (3.21 mm), plant dry matter and roots (27.78 and 52.92 mg, respectively). The greater the amount of sucrose and activated charcoal, the greater the growth. Shorter length was observed in the plants without the addition of activated charcoal. Therefore, to have greater growth, it is suggested to in vitro culture of L. autumnalis seedlings in MS medium with 45 g L-1 of sucrose in combination with 1 g L-1 of activated charcoal, and those of E. cordigera in MS medium supplemented with 45 g L-1 of sucrose and 3 g L-1 of activated charcoal.

Keywords : orchids; carbohydrates; growth.

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