Resumen

ARZATE-FERNANDEZ, Amaury M. et al. Effect of organic complexes on micropropagation of Phalaenopsis var. Dudu. Polibotánica [online]. 2025, n.60, pp.263-272.  Epub 10-Nov-2025. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.60.15.

Phalaenopsis species, belonging to the Orchidaceae family, are popular ornamental plants worldwide due to their elegant appearance and long lifespan, making them a resource of great economic importance for the floral industry. Therefore, it is essential to develop efficient protocols for the micropropagation of both these species and the genus as a whole. Traditionally, the addition of auxins and cytokinins has been the method used to regulate the growth and development of plant tissues, although their use can be expensive. In this context, the aim of this work was to evaluate the effect of peanut beetle feces (HEM), coconut water (AC), and pulque (PUL) on the proliferation of protocorm-like bodies (PLBs) and on the regeneration of Phalaenopsis var. Dudu plants. Clusters of five PLBs were used as explants, with a trifactorial arrangement (5x2x2) that included the following levels: factor A with five levels (0, 2.5, 5.0, 7.5, and 10 g L-1 of HEM), factor B with two levels (0- and 100-mL L-1 AC), and factor C with two levels (0- and 10-mL L-1 PUL). The highest number of PLBs (22.2) was obtained in the treatment with 7.5 g L-1 of HEM and 100 mL L-1 of AC, while the highest number of regenerated plants (5.3) was achieved in the treatment without the addition of organic substitutes, followed by the treatment with 7.5 g L-1 of HEM and 100 mL L-1 of AC (3.7). These results suggest that the combination of peanut beetle feces and coconut water favors the proliferation of PLBs in Phalaenopsis var. Dudu.

Palabras llave : Phalaenopsis; propagation; Ulomoides dermestoides; antioxidants.

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