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Polibotánica

Print version ISSN 1405-2768

Abstract

GOMEZ-SERRANO, Gabriela; CRISTIANI-URBINA, Eliseo  and  VILLEGAS-GARRIDO, Thelma Lilia. Establishment of protocols for in vitro propagation of Acourtia cordata (Cerv.) Turner (Compositae), plants collected in the Sierra de Guadalupe. Polibotánica [online]. 2010, n.30, pp.89-110. ISSN 1405-2768.

The Guadalupe Range (GR) is one of several volcanic complexes within the Mexico Basin. Located in the north-central portion of the basin, GR is now a Protected Natural Area. Therefore, it has become a strategic zone of major importance within the region, for which efforts to preserve its native flora will determine range sustainability. The objective of this work was to establish in vitro plant regeneration protocols for Acourtia cordata, a plant known since pre-Columbian times for its medicinal properties and nowadays for the presence of a dye in its roots. Plants were collected at two localities of the GR and propagated through apical and axillary bud culture, as well as the induction of morphogenetic processes. Two protocols for in vitro plant multiplication were developed; the explants came from buds, leaves and internodes of donor plants obtained from in vitro germination of seeds collected from wild plants. In one protocol plants were formed after indirect shoot organogenesis induced in MS medium containing 5 mg/L of IAA and 0.5 of BAP, followed by rooting in presence of the auxin IBA. In the second one, plants were regenerated by rooting the shoots developed from apical and axillary buds in the same medium used in the morphogenetic processes. The combination of both protocols yielded, from each seed germinated in vitro (germination indices were practically 100%), an average of 22 ± 2 plantlets after 6.5 months, ready for their ex vitro transfer. Results of this study support the importance of the use of in vitro culture systems for the conservation and propagation of valuable genetic resources such as A. cordata, for which there have been no previously reported protocols for in vitro propagation. Such propagation methods can be a valuable tool for the enhancement of natural habitats, to say nothing of their potential to multiply genotypes of useful plants that are sources of technologically important secondary metabolites without damaging their natural populations.

Keywords : Acourtia; Perezia; in vitro plant culture; micropropagation; Guadalupe Range.

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