Abstract

TELLEZ-CASAS, J. Manuel; LOPEZ-PERALTA, Ma. Cristina G.; HERNANDEZ-MENESES, Eleodoro  and  CRUZ-HUERTA, Nicacio. Direct shoot organogenesis from in vitro germinated seedlings of Rhyncholaelia glauca (Lindley) Schlechter. Rev. fitotec. mex [online]. 2024, vol.47, n.3, pp.293-300.  Epub Mar 04, 2025. ISSN 0187-7380.  https://doi.org/10.35196/rfm.2024.3.293.

Rhyncholaelia glauca (Lindley) Schlechter, included in the CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) is a Mexican epiphytic orchid of extraordinary floricultural and genetic potential. In vitro plant tissue culture techniques provide a viable alternative for the preservation and large-scale multiplication of endangered species. The objective of this research was to establish a protocol for the induction of direct organogenesis of shoots from seedlings germinated in vitro. The process started with germination of seeds from a mature, closed capsule. Organogenesis was evaluated in 100 % MS medium with 6-benzyladenine (BA, 1.0-3.0 mg L-1) and 1-naphthaleneacetic acid (NAA, 0.25 and 0.5 mg L-1) with activated charcoal (1.0 g L-1). In multiplication, shoots (1.5-2 cm) were grown on MS medium (50 and 100 %) with BA (2.0-3.0 mg L-1) and NAA or indoleacetic acid (IAA, 0.5 mg L-1) with activated charcoal (0.5 g L-1). In elongation, groups of shoots were placed in MS medium (50 and 100 %) with or without activated charcoal (0.5 g L-1). The assays were placed in a completely randomized experimental design and analysis of variance was used to analyze the data. The highest percentage of germination (90.48 %) was obtained with MS medium 50 % without growth regulators. The highest number of shoots in induction (2.48) and multiplication (3.10) was achieved with 2.5 mg L-1 of BA combined with 0.5 mg L-1 of NAA and 3.0 mg L-1 of BA combined with 0.5 mg L-1 of IAA, respectively. The longest shoot length was 1.2 cm on MS medium 50 % added with 0.5 g L-1 of activated charcoal. The knowledge generated in this research offers an alternative of propagation for sustainable use of Rhyncholaelia glauca to counteract its vulnerability status.

Keywords : Rhyncholaelia glauca; endemic; in vitro germination; organogenesis.

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