Background/Objective.

The objective was to determine the inhibitory effect of actinobacterial morphotypes on mycelial growth of fungi associated with wheat leaf blight.

Materials and methods.

A total of 136 samples of wheat leaves were processed from the highlands of Mexico. They were sectioned, washed and placed in a humid chamber; fungi were isolated on potato dextrose agar. A morphological identification was carried out and the incidence of diseases was recorded by federal entity. Actinobacteria were isolated from soil samples using the dilution technique and microcolonies were isolated on nutrient agar. Preliminary in vitro bioassays were established at 27 °C with 144 actinobacteria and a dual confrotation with morphotypes presented a percentage of inhibition of mycelial growth (PIMG) greater than 40% and to identify those with potential for the control of fungi associated with leaf spots. The experimental design was completely randomized and an analysis of variance (p ≤ 0.05) and mean test (Tukey p ≤ 0.05) were performed.

Results.

At least three actinobacteria inhibited mycelial growth of Fusarium spp. in a range of 42.5 - 66.9 %. The inhibitory efficacy of strain M2B M60 was highest with Fusarium isolate 1, that of M1B33 with Fusarium isolate 2 and that of M1B M4 was most effective with Fusarium isolate 3.

Conclusion.

The use of actinobacteria represents an alternative for integrated management in the control of fungi associated with foliar blight in wheat, as they present an inhibitory effect on mycelial growth.

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