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Revista mexicana de fitopatología

On-line version ISSN 2007-8080Print version ISSN 0185-3309


MEDINA GOMEZ, Edgar; RAMIREZ SUAREZ, Angel; CUEVAS OJEDA, Juventino  and  MARTINEZ GOMEZ, Daniel. Identification and phylogenetic analysis of the leaf-galling nematode Orrina phyllobia affecting Solanum elaeagnifolium Cav. in Guanajuato, Mexico. Rev. mex. fitopatol [online]. 2016, vol.34, n.2, pp.184-199. ISSN 2007-8080.

The silver-leaf nightshade Solanum elaeagnifolium is an economic importance weed affected by natural enemies that would be used as biological control agent. The leaf-galling nematode Orrina phyllobia was detected parasitizing S. elaeagnifolium in San Luis de la Paz, Guanajuato. Morphotaxonomic and molecular analysis was performed on specimens extracted from foliar galls and distortioned leaves. The morphometric values were in agreement with those reported by the original species description except with de Man ratios: a and b. The Polymerase Chain Reaction amplification products of two molecular markers ITS1-5.8S-ITS2 and the D2-D3 expansion segments of 28S gene were 850 and 900 bp respectively. The homology by BLASTING of the mexican population genetic sequences together with those from NCBI database showed 99% similarity with Ditylenchus phyllobius (E=0.0). The bayesian inference of the ribosomal DNA marker ITS1-5.8S-ITS2 with sequences of genus and anguinid species uploaded in GenBank displayed a clear grouping affinity with Ditylenchus phyllobius. The combination of morphometric and genetic characters allowed to confirm the identity of O. phyllobia atacking S. elaeagnifolium in Mexico.

Keywords : Biocontrol; nematodes; weeds; sequencing; phylogeny.

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