Resumo

QUI-ZAPATA, Joaquín Alejandro; PENA-RODRIGUEZ, Luis Manuel; CASTRO-CONCHA, Lizbeth Arianelly  e  MIRANDA-HAM, María de Lourdes. Lipid Peroxidation as a Marker of Cell Death in Cell Cultures of Mexican Marigold (Tagetes erecta L.). Rev. mex. fitopatol [online]. 2010, vol.28, n.2, pp.165-167. ISSN 2007-8080.

Lipid peroxidation in plants is an important feature of hypersensitive cell death during incompatible or non-host interactions of plants with pathogens. Malondialdehyde is a marker of oxidative lipid injury. The 1 -methyl-2-phenylindole colorimetric assay has been considered specific for the quantitation of malondialdehyde in animal systems, but its applicability to plant tissues remains unknown. In here, we report its utilization in cell cultures of Tagetes erecta L. (cempasúchil) treated with hydrogen peroxide. Our results suggest a close relationship between the extent of membrane damage that resulted from lipid peroxidation and cell viability.

Palavras-chave : Colorimetric assay; lipid peroxidation; malondialdehyde; oxidative stress.

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