Abstract

GONZALEZ-CERON, Lilia; RODRIGUEZ, Mario H; BETANZOS, Angel F  and  ABADIA, Acatl. Eficacia de una prueba rápida para el diagnóstico de Plasmodium vivax en pacientes sintomáticos de Chiapas, México. Salud pública Méx [online]. 2005, vol.47, n.4, pp.282-287. ISSN 0036-3634.

OBJECTIVE: To evaluate, under laboratory conditions, the sensitivity and specificity of a rapid diagnostic test (OptiMAL), based on immunoreactive strips, to detect Plasmodium vivax infection in febrile patients in Southern Chiapas, Mexico. MATERIAL AND METHODS: The presence of parasites in blood samples of 893 patients was investigated by Giemsa-stained thick blood smear microscopic examination (gold standard). A blood drop from the same sample was smeared on immunoreactive strips to investigate the presence of the parasite pLDH. Discordant results were resolved by PCR amplification of the parasite's 18S SSU rRNA, to discard infection. RESULTS: OptiMAL had an overall sensitivity of 93.3% and its specificity was 99.5%. Its positive and negative predictive values were 96.5% and 98.9%, respectively. Signal intensity in OptiMAL strips correlated well with the parasitemia density in the blood samples (r= 0.601, p= 0.0001). CONCLUSIONS: This rapid test had acceptable sensitivity and specificity to detect P. vivax under laboratory conditions and could be useful for malaria diagnosis in field operations in Mexico.

Keywords : Plasmodium vivax; diagnosis; rapid test; OptiMAL; thick blood smear; Mexico.

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