Resumen

SANCHEZ-ESCAMILLA, José Luis et al. Ostespheroid caracterization to promote mineralization. Mundo nano [online]. 2025, vol.18, n.34, e69829.  Epub 25-Feb-2025. ISSN 2448-5691.  https://doi.org/10.22201/ceiich.24485691e.2025.34.69829.

This work shows the obtaining of osteospheroids, which have the ability to produce mineralized nodules, which can be used as study models to test nanodrugs or nanomaterials. Cell suspension cultures were made to obtain fetal osteoblast spheroids, with different cell concentrations/mL for 5 days. The condition where the diameter ranges from 80 to 150 µM was selected to make tests at 3, 7, 14 and 21 days. The cell viability of the osteospheroids was quantified by trypan blue exclusion and clonogenic assays were realized to determine the effect of the mineralizing medium on the formation of these structures. The integrity of the osteospheroids was observed by H&E and the formation of mineralized nodules was detected by alizarin red staining. The results show regular osteospheroids without aggregates at a concentration of 5 x 102 cells/mL and viable above 70% after 7 days of formation. Clonogenic assays do not show significant differences in morphology or the number of colonies between the control and the medium that induces mineralization. H&E stains show nuclei and defined cytoplasm at 3, 7, and 14 days, and at 7 and 14 days, alizarin red staining suggests that they are generating calcium deposits.

Palabras llave : osteospheroids; 3D culture; mineralized nodules; stem cell.

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