Resumen

HERNANDEZ HERNANDEZ, Rosa Elena et al. Evaluation of two substrate disinfection methods for the production of Pleurotus ostreatus. Polibotánica [online]. 2025, n.60, pp.355-366.  Epub 10-Nov-2025. ISSN 1405-2768.  https://doi.org/10.18387/polibotanica.60.21.

The objective of this work was to evaluate two disinfection methods for corn stover (M) and sorghum straw (S) substrates used as substrates for the production of Pleurotus ostreatus, in a rustic manner. Two substrate disinfection methods were tested: a) water with calcium oxide (CaO) (400 g) and hydrated calcium sulfate (CaSO4 2H2O) (400 g) plus thermal application (T1M and T5S), b) water with CaO (400 g) and CaSO4 2H2O (400 g) without thermal application (T2M and T6S), c) water with commercial sodium hypochlorite (NaClO) (400 ml) plus thermal application (T3M and T7S) and d) water with NaClO (400 ml) without thermal application (T4M and T8S), both for corn stover and sorghum stover. Eight treatments were obtained, each with five replicates. Thermal application was a very important factor in disinfection, because treatments with this method presented a lower percentage of contamination (T1M, T3M, T5S and T7S), contrary to those that did not have thermal application, which were 100% contaminated; except for T2M with 43%, with respect to the total number of bags planted. From the uncontaminated ones, carpophore harvests could be obtained; a greater quantity of primordia was obtained in T3M (65) and T1M (48), greater carpophore production in T7S and T3M with a total weight of 87.33 g and 85.25 g on average, respectively. The length of the carpophore stipe was greater in T7S with 3.94 cm. The diameter of the carpophore stipe was greater in T7S with 1 cm. It is recommended that for the production of P. ostreatus in a rustic and small-scale manner, agricultural residues available in the region be used, in addition to the disinfection of the substrate through thermal treatment, with the addition of NaClO or with CaO and CaSO4 2H2O to the water.

Palabras llave : Disinfection; Edible mushrooms; Mycelium; Production; Agricultural waste.

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