ESCAMINA-LOZANO, Y. et al. Synthesis of α-L-Fucosidase in different strains of lactic acid bacteria. []. , 14, 3, pp.623-629. ISSN 1665-2738.

^len^aThe ability of six lactic acid bacteria to produce α-L-fucosidase is reported here for the first time, opening a wide field of investigation into the metabolism and assimilation of human milk oligosaccharides by lactic acid bacteria. Lactobacillus casei IMAU60214, Lactobacillus casei Shirota, Lactobacillus rhamnosus GG, Lactobacillus rhamnosus KLDS, Lactobacillus helveticus IMAU70129 and Lactobacillus delbrueckii subsp. bulgaricus NCFB-2772 were all able to produce α-L-fucosidases. Growth kinetics and carbohydrate consumption meas urements indicated that the six strains were able to metabolise D-glucose and D-galactose as a carbon source; surprisingly, they did not assimilate L-fucose. However, α-L-fucosidase was a cell-associated enzyme and produced constitutively in different carbon sources. The highest cell-associated α-L-fucosidase activity was observed in L. rhamnosus GG (0.16 U mg^-1).^les^aEste es el primer estudio que reporta las síntesis de α-L-fucosidasa en seis bacterias ácido lácticas, lo que abre un amplio campo de investigación sobre el metabolismo y la asimilación de los oligosacáridos de la leche humanos por parte de estos microorganismos. Lactobacillus casei IMAU60214, Lactobacillus casei Shirota, Lactobacillus rhamnosus GG, Lactobacillus rhamnosus KLDS, Lactobacillus helveticus IMAU70129 y Lactobacillus delbrueckii subsp. bulgaricus NCFB-2772 fueron capaces de producir α-L-fucosidasa. Las cinéticas de crecimiento y el consumo de carbohidratos indican que las seis cepas son capaces de metabolizar D-glucosa y D-galactosa como fuente de carbono; pero no asimilaron L-fucosa. Por otro lado, las α-L-fucosidasas sintetizadas son enzimas asociadas a la célula y se produjeron de manera constitutiva en diferentes fuentes de carbono. La mayor actividad enzimática se observó en L. rhamnosus GG (0.16 U mg^-1).

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