<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-4298</journal-id>
<journal-title><![CDATA[Botanical Sciences]]></journal-title>
<abbrev-journal-title><![CDATA[Bot. sci]]></abbrev-journal-title>
<issn>2007-4298</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Botánica de México A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-42982018000400678</article-id>
<article-id pub-id-type="doi">10.17129/botsci.1924</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Lacandonia granules are present in the cell nucleus of Welwitschia mirabilis]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Agredano-Moreno]]></surname>
<given-names><![CDATA[Lourdes Teresa]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Segura-Valdez]]></surname>
<given-names><![CDATA[María de Lourdes]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Jiménez-Ramírez]]></surname>
<given-names><![CDATA[Jaime]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Jiménez-García]]></surname>
<given-names><![CDATA[Luis Felipe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,National Autonomous University of Mexico Faculty of Sciences ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,National Autonomous University of Mexico Faculty of Sciences Department of Cell Biology]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,National Autonomous University of Mexico Faculty of Sciences Department of Comparative Biology]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<volume>96</volume>
<numero>4</numero>
<fpage>678</fpage>
<lpage>683</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-42982018000400678&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-42982018000400678&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-42982018000400678&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract:  Background: Lacandonia granules are extranucleolar ribonucleoprotein (RNPs) particles, 32 nanometers in diameter that were first described in the nucleus of Lacandonia schismatica. Cytochemical and immunocytochemical studies suggest that these particles are equivalent to perichromatin and Balbiani ring granules described in mammals and salivary glands cells of the insect Chironomus tentans, respectively. Lacandonia granules are also present in the related Triuris brevystilis, and they were later described in the gymnosperm Ginkgo biloba. These findings suggest that Lacandonia granules have a wider distribution in the plant kingdom.  Species study: Welwitschia mirabilis, a gymnosperm of the order Gnetales.  Hyphotesis: Lacandonia granules are present in the cell nucleus of W. mirabilis.  Methods: Plants were cultivated in a germination chamber and samples of leaves were processed for transmission electron microscope. Thin sections were stained with the EDTA technique preferential for ribonucleoproteins and osmium amine specific for DNA and observed with an electron microscope.  Results: Light, electronic and atomic force microscopy revealed that cell nuclei of W. mirabilis display a reticulated arrangement of chromatin. Moreover, granules of 32.17 ± 1.7 nm in diameter were observed among strands of reticulated chromatin.  Conclusions: Our results indicate that Lacandonia granules are present in the nuclei of the gnetal W. mirabilis.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen:  Antecedentes: los gránulos de Lacandonia son partículas ribonucleoproteicas extranucleolares (RNPs), de 32 nanómetros de diámetro, que se describieron por primera vez en el núcleo de Lacandonia schismatica. Los estudios citoquímicos e inmunoquímicos sugieren que estas partículas son equivalentes a los gránulos pericromatinianos y de los anillos de Balbiani descritos en mamíferos y células de glándulas salivales del insecto Chironomus tentans, respectivamente. Los gránulos de Lacandonia también están presentes en Triuris brevystilis, y posteriormente se describieron en la gimnosperma Ginkgo biloba. Estos hallazgos sugieren que los gránulos de Lacandonia tienen una distribución más amplia en el reino vegetal.  Especie de estudio: Welwitschia mirabilis, una gimnosperma del orden Gnetales.  Hipotesis: Los gránulos de Lacandonia están presentes en el núcleo celular de W. mirabilis  Métodos: Las plantas se cultivaron en una cámara de germinación y se procesaron muestras de hojas para microscopía electrónica de transmisión. Los cortes delgados se tiñeron con la técnica de EDTA preferencial para ribonucleoproteínas y amina de osmio específicas para ADN y se observaron al microscopio electrónico.  Resultados: La microscopía de luz, electrónica y de fuerza atómica revelaron que los núcleos celulares de W. mirabilis muestran una disposición reticulada de la cromatina. Además, se observaron gránulos de 32.17 ± 1.7 nm de diámetro entre las hebras de cromatina reticulada.  Conclusiones: Nuestros resultados indican que los gránulos de Lacandonia están presentes en los núcleos del gnetal W. mirabilis.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Atomic force microscopy]]></kwd>
<kwd lng="en"><![CDATA[gymnosperms]]></kwd>
<kwd lng="en"><![CDATA[Lacandonia granules]]></kwd>
<kwd lng="en"><![CDATA[plant cell ultrastructure]]></kwd>
<kwd lng="en"><![CDATA[Welwitschia]]></kwd>
<kwd lng="es"><![CDATA[gimnospermas]]></kwd>
<kwd lng="es"><![CDATA[gránulos de Lacandonia]]></kwd>
<kwd lng="es"><![CDATA[microscopía de fuerza atómica]]></kwd>
<kwd lng="es"><![CDATA[ultraestructura de plantas]]></kwd>
<kwd lng="es"><![CDATA[Welwitschia]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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