<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-5337</journal-id>
<journal-title><![CDATA[Perinatología y reproducción humana]]></journal-title>
<abbrev-journal-title><![CDATA[Perinatol. Reprod. Hum.]]></abbrev-journal-title>
<issn>0187-5337</issn>
<publisher>
<publisher-name><![CDATA[Instituto Nacional de Perinatología]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-53372023000300108</article-id>
<article-id pub-id-type="doi">10.24875/per.23000024</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente]]></article-title>
<article-title xml:lang="en"><![CDATA[Multiplex PCR testing for the atypical microorganisms diagnosis sexually transmitted]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Escobedo-Guerra]]></surname>
<given-names><![CDATA[Marcos R.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López-Hurtado]]></surname>
<given-names><![CDATA[Marcela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gutiérrez-Trujillo]]></surname>
<given-names><![CDATA[Rodrigo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bustos-López]]></surname>
<given-names><![CDATA[Abraham D.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Guerra-Infante]]></surname>
<given-names><![CDATA[Fernando M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Nacional de Perinatología Isidro Espinoza de los Reyes Departamento de Infectología Perinatal ]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro Médico Naval Laboratorio de Pruebas Especiales ]]></institution>
<addr-line><![CDATA[Ciudad de México ]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2023</year>
</pub-date>
<volume>37</volume>
<numero>3</numero>
<fpage>108</fpage>
<lpage>114</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-53372023000300108&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-53372023000300108&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-53372023000300108&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen  Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos. Los microorganismos atípicos como Chlamydia trachomatis y micoplasmas no son identificados debido a los requerimientos. Además, no es incluida Gardnerella vaginalis, aunque se asocia a la vaginosis bacteriana.  Objetivo: Desarrollar una PCR múltiplex para el diagnóstico de C. trachomatis, micoplasmas y G. vaginalis.  Método: Se estandarizó la PCR múltiplex utilizando oligonucleótidos para C. trachomatis (gen ompA, orf6 plasmídico), Mycoplasma/Ureaplasma y G. vaginalis (genes rRNA16s).  Resultados: Se estandarizaron pruebas de PCR múltiplex para los microorganismos estudiados, optimizándose las concentraciones y condiciones de las reacciones múltiplex. Se obtuvieron PCR dúplex para C. trachomatis (ompA, orf6), Chlamydia/Gardnerella y Chlamydia/micoplasmas y tríplex para Chlamydia/Mycoplasma/Ureaplasma. También un cuádruplex para Chlamydia/Mycoplasma/Ureaplasma/Gardnerella. Los resultados fueron verificados por PCR e hibridación automática (HybriSpot 12) y análisis in silico.  Conclusión: Se desarrollaron pruebas de PCR múltiplex con una alta sensibilidad y especificidad para la identificación de C. trachomatis, micoplasmas y G. vaginalis.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Background: Sexually transmitted infections are a global public health problem. Routine analysis includes microbiological and serological tests for the diagnosis of pathogens. Atypical microorganisms such as Chlamydia trachomatis and mycoplasmas are not determined due to the requirements for their identification. Furthermore, Gardnerella vaginalis is not included despite being associated with bacterial vaginosis.  Objective: To develop a multiplex PCR to diagnose Chlamydia, mycoplasmas, and Gardnerella.  Method: Standardization of multiplex PCR tests was carried out using oligonucleotides for the identification of Chlamydia (ompA gene, plasmid orf6), Mycoplasma/Ureaplasma and Gardnerella (rRNA16s genes).  Results: Multiplex PCR tests were standardized for the microorganisms studied, optimizing the concentrations and conditions of the multiplex reactions. Duplex PCR was obtained for Chlamydia (ompA, orf6), Chlamydia/Gardnerella, and Chlamydia/mycoplasmas, and triplex PCR for Chlamydia/mycoplasmas. Also, a quadruplex for Chlamydia, Mycoplasma/Ureaplasma and Gardnerella. PCR and automatic hybridization verified the results obtained (HybriSpot 12) and in silico analysis.  Conclusion: Multiplex PCR tests with high sensitivity and specificity were developed to identify C. trachomatis, mycoplasmas, and G. vaginalis.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[PCR múltiplex]]></kwd>
<kwd lng="es"><![CDATA[Chlamydia trachomatis]]></kwd>
<kwd lng="es"><![CDATA[Mycoplasma spp]]></kwd>
<kwd lng="es"><![CDATA[Ureaplasma spp]]></kwd>
<kwd lng="es"><![CDATA[Gardnerella vaginalis]]></kwd>
<kwd lng="es"><![CDATA[Infección de transmisión sexual]]></kwd>
<kwd lng="en"><![CDATA[Multiplex PCR]]></kwd>
<kwd lng="en"><![CDATA[Chlamydia trachomatis]]></kwd>
<kwd lng="en"><![CDATA[Mycoplasma spp]]></kwd>
<kwd lng="en"><![CDATA[Ureaplasma spp]]></kwd>
<kwd lng="en"><![CDATA[Gardnerella vaginalis]]></kwd>
<kwd lng="en"><![CDATA[Sexually transmitted infection]]></kwd>
</kwd-group>
</article-meta>
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