<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092025000100005</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.2310-5</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Diversidad y taxonomía de Fusarium solani aislado de plantas marchitas de Agave tequilana var azul]]></article-title>
<article-title xml:lang="en"><![CDATA[Diversity and taxonomy of Fusarium solani isolated of wilted Agave tequilana var. azul plants]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Montaño-Becerrra]]></surname>
<given-names><![CDATA[Viviana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mancilla-Margalli]]></surname>
<given-names><![CDATA[Norma Alejandra]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chávez-Sánchez]]></surname>
<given-names><![CDATA[Cristina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Avila-Miranda]]></surname>
<given-names><![CDATA[Martin Eduardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Tecnológico Nacional de México  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Tecnológico Nacional de México  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2025</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2025</year>
</pub-date>
<volume>43</volume>
<numero>1</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092025000100005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092025000100005&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092025000100005&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN   Antecedentes/Objetivo. El objetivo del trabajo fue identificar 24 cepas de F. solani aisladas de agave con marchitez, respecto a las nuevas especies filogenéticas; de- terminar su semejanza molecular con f. spp. de F. solani; determinar su diversidad genética y su capacidad patogénica en agave, frijol (Phaseolus vulgaris) y maíz (Zea mays).   Materiales y Métodos. Secuencias del fragmento ITS1-5.8S-ITS2 de 24 aislados de agave y de las f.spp. de F. solani, se compararon con el GenBank y FUSAROID- ID. Secuencias amplificadas del 18S rRNA, se alinearon con secuencias reportadas de F. solani f. spp. phaseoli y batatas, definiendo presencia de intrones. Se deter- minó diversidad genética con el marcador de DNA RepPCR. Cepas representativas se confrontaron con plántulas de agave, frijol y maíz, evaluando su patogenicidad como severidad de pudrición radicular.   Resultados. Aislados morfológicamente identificados como F. solani, el GenBank los ubicó como F. solani o incluidos en el FSSC, tres cepas se identificaron como Xenoacremonium sp. FUSAROID-ID definió que las secuencias de F. solani eran altamente similares a las de Neocosmospora martii, N. pseudoradicicola, N. solani y N. falciformis. Las secuencias ITS1-5.8S-ITS2 y ausencia de intrones en su SSU, indicó que ninguno es F. solani f.sp. phaseoli. Aislados obtenidos de agave fueron patogénicos a A. tequilana y a un cv de maíz criollo, pero no a maíces con resisten- cia a Fusarium. Ningún aislado de agave fue patogénico a frijol.  Conclusiones. Cuatro especies filogenéticas del FSSC provocan pudrición radi- cular en agave; aislados de F. solani de agave no afectaron a maíces resistentes a Fusarium. Es seguro intercalar frijol en el cultivo de agave.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract  Background/Objective. The objective of this work was to identify 24 strains of F. solani isolated from agave with wilt, with respect to the new phylogenetic species; determine their molecular similarity to F. solani f. spp.; determine their genetic diversity and their pathogenic capacity in agave, bean and corn.   Materials and Methods. Sequences of the ITS1-5.8S-ITS2 fragment of 24 agave isolates and those of F. solani f.spp., were compared with GenBank and FUSAROID-ID. Amplified 18S rRNA sequences were aligned with sequences reported of F. solani f. spp. phaseoli and batatas, defining the presence of introns. Genetic diversity was determined with the DNA RepPCR marker. Representative strains were tested against agave, bean and maize seedlings, evaluating their pathogenicity as root rot severity.   Results. Isolates morphologically identified as F. solani, GenBank placed them as F. solani or included in the FSSC, three strains were identified as Xenoacremonium sp. FUSAROID-ID defined that the sequences of F. solani were highly similar to those of Neocosmospora martii, N. pseudoradicicola, N. solani and N. falciformis. The ITS1-5.8S-ITS2 sequences and absence of introns in its SSU indicated that none is F. solani f. sp. phaseoli. Isolates obtained from agave were pathogenic to A. tequilana and a criollo corn cv, but not to Fusarium-resistant corn. No agave isolates were pathogenic to beans.   Conclusions. Four phylogenetic species of FSSC cause root rot in agave; F. solani isolates from agave did not affect Fusarium-resistant corn. It is safe to intercrop beans in agave.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Neocosmospora]]></kwd>
<kwd lng="es"><![CDATA[Rep-PCR]]></kwd>
<kwd lng="es"><![CDATA[f. sp.]]></kwd>
<kwd lng="es"><![CDATA[phaseoli]]></kwd>
<kwd lng="en"><![CDATA[Neocosmospora]]></kwd>
<kwd lng="en"><![CDATA[Rep-PCR]]></kwd>
<kwd lng="en"><![CDATA[f. sp. phaseoli.]]></kwd>
</kwd-group>
</article-meta>
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