<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092016000200131</article-id>
<article-id pub-id-type="doi">10.18781/R.MEX.FIT.1601-1</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Detección de Pineapple mealybug wilt-associated virus 1 y 3 en México]]></article-title>
<article-title xml:lang="en"><![CDATA[Detection of Pineapple mealybug wilt-associated virus 1 and 3 in Mexico]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ochoa-Martínez]]></surname>
<given-names><![CDATA[Daniel Leobardo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Uriza-Ávila]]></surname>
<given-names><![CDATA[Daniel Emigdio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rojas-Martínez]]></surname>
<given-names><![CDATA[Reyna Isabel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez-Martínez]]></surname>
<given-names><![CDATA[Douglas]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Colegio de Postgraduados Campus Montecillo Postgrado en Fitosanidad-Fitopatología]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
</aff>
<aff id="Af2">
<institution><![CDATA[,INIFAP Campo Experimental Cotaxtla ]]></institution>
<addr-line><![CDATA[ Ver.]]></addr-line>
</aff>
<aff id="Af3">
<institution><![CDATA[,Colegio de Postgraduados Campus Montecillo Postgrado en Fitosanidad-Fitopatología]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2016</year>
</pub-date>
<volume>34</volume>
<numero>2</numero>
<fpage>131</fpage>
<lpage>141</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092016000200131&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092016000200131&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092016000200131&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: En El Bajo Papaloapan, principal zona productora de piña, se colectaron hojas con síntomas de clorosis, flacidez, reducción del crecimiento y enrojecimiento foliar típicos de infecciones virales en este cultivo. Mediante RT-PCR con indicadores específicos para el gen hsp70 y posterior secuenciación, fueron detectados los virus Pineapple mealybug wilt associated-virus 1 (PMWaV-1) y Pineapple mealybug wilt associated-virus 3 (PMWaV-3). A partir de las secuencias obtenidas se construyó un árbol con secuencias procedentes de diferentes regiones del mundo disponibles en el GenBank para determinar su similitud. La secuencia obtenida del aislamiento mexicano del PMWaV-1 resultó ser más próxima genéticamente a secuencias de aislamientos procedentes de Cuba, Taiwán, Tailandia y Hawai y más distante del aislamiento australiano. La secuencia obtenida para el aislamiento mexicano del PMWaV-3 se encontró más relacionada con los aislamientos de Hawai, Cuba, Australia y Taiwán y más distante del aislamiento tailandés. Este es el primer reporte de la presencia de estos dos virus en México.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: In El Bajo Papaloapan, the main producing area of pineapple of Mexico, leaves with typical symptoms of viral infection consisting in chlorosis, flaccidity, reduced growth and reddening were collected. By RT-PCR with specific primers for the hsp70 gene and subsequent sequencing were detected Pineapple mealybug wilt virus associated-virus 1 (PMWaV-1) and Pineapple mealybug wilt virus associated-3 (PMWaV-3). From the sequences obtained a tree was done with sequences from different regions of the world available in GenBank in order to know their similarity. The sequence obtained from the Mexican isolate PMWaV-1 was genetically related to the sequences of isolates from Cuba, Taiwan, Thailand and Hawaii and more distant from the Australian isolate. The sequence obtained for the Mexican isolate PMWaV-3 was more related to isolates from Hawaii, Cuba, Australia and Taiwan and more distant from the Thailand isolate. This is the first report of the presence of these two viruses in Mexico.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[ampelovirus]]></kwd>
<kwd lng="es"><![CDATA[piojo harinoso]]></kwd>
<kwd lng="es"><![CDATA[marchitez roja de la piña]]></kwd>
<kwd lng="es"><![CDATA[filogenia]]></kwd>
<kwd lng="en"><![CDATA[ampeloviruses]]></kwd>
<kwd lng="en"><![CDATA[mealybug]]></kwd>
<kwd lng="en"><![CDATA[pineapple red wilt]]></kwd>
<kwd lng="en"><![CDATA[phylogeny]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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