<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-0934</journal-id>
<journal-title><![CDATA[Revista mexicana de ciencias agrícolas]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Mex. Cienc. Agríc]]></abbrev-journal-title>
<issn>2007-0934</issn>
<publisher>
<publisher-name><![CDATA[Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-09342016000701759</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Capacidad de sobrevivencia de tres especies de Phytophthora y dos de Pythium preservados en dos sustratos a corto y largo plazo]]></article-title>
<article-title xml:lang="en"><![CDATA[Survivability of three species of Phytophthora and two of Pythium substrates preserved in short and long term]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Molina-Gayosso]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Andrade-Hoyos]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Espinosa]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Sosa-Hernández]]></surname>
<given-names><![CDATA[C. M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Politécnica de Puebla  ]]></institution>
<addr-line><![CDATA[Puebla ]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Politécnica  ]]></institution>
<addr-line><![CDATA[Francisco I. Madero Hidalgo]]></addr-line>
</aff>
<aff id="Af3">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Montecillos ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>11</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>11</month>
<year>2016</year>
</pub-date>
<volume>7</volume>
<numero>7</numero>
<fpage>1759</fpage>
<lpage>1764</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-09342016000701759&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-09342016000701759&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-09342016000701759&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen El mantenimiento y viabilidad de microorganismos preservados en las colecciones es un problema común en laboratorios de diagnóstico de enfermedades de las plantas e instituciones de investigación, y más aún, en aquellas en donde se incluyen a especies de los géneros Phytophthora y Pythium y en el que la criopreservación no es una opción. La conservación de oomicetos en agua estéril es un método económico y confiable. Se han reportado periodos de conservación de hasta 23 años para especies de Phytophthora. En el presente trabajo se evaluó la capacidad de sobrevivencia y viabilidad de especies de Phytophthora y Pythium mantenidos en suelo por periodos de 3 a 7 años y en agua estéril por periodos de 6 a 21 años. La viabilidad se confirmó mediante siembra en medio de cultivo harina de maíz-agar suplementado con antibióticos. Ninguno de los oomicetos conservados en suelo sobrevivió durante los periodos de conservación mientras que la conservación en agua estéril mantuvo viables a especies de Phytophthora por periodos de hasta 21 años y de Pythium por 7 años.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract The maintenance and viability of microorganisms preserved in the collections is a common problem in laboratory diagnosis of plant diseases and research institutions, and even in those where they include species of the genera Phytophthora and Pythium and in which cryopreservation is not an option. The oomycetes conservation in sterile water is an economic and reliable method. They have been reported retention periods up to 23 years for species of Phytophthora. In this work were evaluated the ability to survival and viability of Phytophthora and Pythium species kept in soil for periods of three to seven years in sterile water for periods of 6 to 21 years. Viability was confirmed by plating on medium cornmeal-agar culture medium supplemented with antibiotics. None of the oomycetes survived preserved in soil conservation during periods while in sterile water conservation remained viable Phytophthora species for up to 21 years and Pythium for 7 years.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[antibióticos]]></kwd>
<kwd lng="es"><![CDATA[criopreservación]]></kwd>
<kwd lng="es"><![CDATA[maíz-agar]]></kwd>
<kwd lng="es"><![CDATA[microorganismos]]></kwd>
<kwd lng="es"><![CDATA[oomicetos]]></kwd>
<kwd lng="en"><![CDATA[antibiotics]]></kwd>
<kwd lng="en"><![CDATA[corn-agar]]></kwd>
<kwd lng="en"><![CDATA[cryopreservation]]></kwd>
<kwd lng="en"><![CDATA[microorganisms]]></kwd>
<kwd lng="en"><![CDATA[oomicetos]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Boesewinkel]]></surname>
<given-names><![CDATA[H. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Storage of fungal cultures in water]]></article-title>
<source><![CDATA[Trans. Br. Mycol. Soc.]]></source>
<year>1976</year>
<volume>66</volume>
<page-range>183-5</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Borman]]></surname>
<given-names><![CDATA[M. A.]]></given-names>
</name>
<name>
<surname><![CDATA[Szekely]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Campbell]]></surname>
<given-names><![CDATA[C. K.]]></given-names>
</name>
<name>
<surname><![CDATA[Johnson]]></surname>
<given-names><![CDATA[M. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Evaluation of the viability of pathogenic filomentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods]]></article-title>
<source><![CDATA[Mycopathologia.]]></source>
<year>2006</year>
<volume>161</volume>
<page-range>361-8</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Coffey]]></surname>
<given-names><![CDATA[D. M. D.]]></given-names>
</name>
</person-group>
<source><![CDATA[The world oomycetes genetic resource collection. Third international workshop Phytophthora/Pythium and related genera. Slide presentation]]></source>
<year>2008</year>
</nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dahmen]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
<name>
<surname><![CDATA[Staub]]></surname>
<given-names><![CDATA[Th.]]></given-names>
</name>
<name>
<surname><![CDATA[Schwinn]]></surname>
<given-names><![CDATA[F. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Technique for long-term preservation of phytopathogenic fungi in liquid nitrogen]]></article-title>
<source><![CDATA[Phytopathology.]]></source>
<year>1983</year>
<volume>73</volume>
<page-range>241-6</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dhingra]]></surname>
<given-names><![CDATA[D. O.]]></given-names>
</name>
<name>
<surname><![CDATA[Sinclair]]></surname>
<given-names><![CDATA[J. B.]]></given-names>
</name>
</person-group>
<source><![CDATA[Basic plant pathology methods]]></source>
<year>1987</year>
<page-range>49</page-range><publisher-loc><![CDATA[Boca Raton, Florida ]]></publisher-loc>
<publisher-name><![CDATA[CRC. Press, Inc]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Elliot]]></surname>
<given-names><![CDATA[C. G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Physiology of sexual reproduction in Phytophthora]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Erwin]]></surname>
<given-names><![CDATA[D. C.]]></given-names>
</name>
<name>
<surname><![CDATA[Bartnicki-García]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Tsao]]></surname>
<given-names><![CDATA[P. H.]]></given-names>
</name>
</person-group>
<source><![CDATA[Phytophthora: its biology, taxonomy, ecology and pathology]]></source>
<year>1983</year>
<page-range>71-80</page-range><publisher-loc><![CDATA[St. Paul Minnesota ]]></publisher-loc>
<publisher-name><![CDATA[APS Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Heckely]]></surname>
<given-names><![CDATA[R. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Preservation of microorganisms]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Perlaman]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<source><![CDATA[Advances in applied microbiology]]></source>
<year>1978</year>
<volume>24</volume>
<page-range>1-53</page-range><publisher-name><![CDATA[Academic Press Inc.]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Humber]]></surname>
<given-names><![CDATA[R.A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fungi: preservation of cultures]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Lacery]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[manual of techniques in insect pathology]]></source>
<year>1997</year>
<page-range>409</page-range><publisher-name><![CDATA[Academic Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Judelson]]></surname>
<given-names><![CDATA[H. S.]]></given-names>
</name>
<name>
<surname><![CDATA[Blanco]]></surname>
<given-names><![CDATA[F.A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The spores of Phytophthora: weapons of the plant destroyer]]></article-title>
<source><![CDATA[Nature Reviews. Microbiology.]]></source>
<year>2005</year>
<volume>3</volume>
<page-range>47-58</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ko]]></surname>
<given-names><![CDATA[W. H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Long-term storage and survival structure of three species of Phytophthora in water]]></article-title>
<source><![CDATA[J. General Plant Pathol.]]></source>
<year>2003</year>
<volume>69</volume>
<page-range>186-8</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ribeiro]]></surname>
<given-names><![CDATA[O. K.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Physiology of asexual sporulation and spore germination in Phytophthora]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Erwin]]></surname>
<given-names><![CDATA[D. C.]]></given-names>
</name>
<name>
<surname><![CDATA[Bartnicki-García]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Tsao]]></surname>
<given-names><![CDATA[P. H.]]></given-names>
</name>
</person-group>
<source><![CDATA[Phytophthora: its biology, taxonomy, ecology and pathology]]></source>
<year>1983</year>
<page-range>55-70</page-range><publisher-loc><![CDATA[St. Paul Minnesota ]]></publisher-loc>
<publisher-name><![CDATA[APS Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Richter]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
<name>
<surname><![CDATA[Bruhn]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Revival of saprotrophic and mycorrhizal basidiomycetes culture from cold storage in sterile water]]></article-title>
<source><![CDATA[Can. J. Microbiol.]]></source>
<year>1989</year>
<volume>35</volume>
<page-range>1055-60</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ryan]]></surname>
<given-names><![CDATA[M. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Smith]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Jeffries]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A decision-based key to determine the most appropriate protocol for the preservation of fungi]]></article-title>
<source><![CDATA[World J. Microbiol. Biotechnol.]]></source>
<year>2000</year>
<volume>16</volume>
<page-range>183-6</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Simpfendorfer]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
<name>
<surname><![CDATA[Harden]]></surname>
<given-names><![CDATA[T. J.]]></given-names>
</name>
<name>
<surname><![CDATA[Murray]]></surname>
<given-names><![CDATA[G. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Viability and pathogenicity of Phytophthora clandestina after storage in water and liquid nitrogen]]></article-title>
<source><![CDATA[Aust. Plant Pathol.]]></source>
<year>1996</year>
<volume>25</volume>
<page-range>234-9</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Smith]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Liquid nitrogen storage of fungi]]></article-title>
<source><![CDATA[Transactions of the British Mycological Society.]]></source>
<year>1982</year>
<volume>79</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>415-21</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tsao]]></surname>
<given-names><![CDATA[H. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Ocana]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Selective isolation of species of Phytophthora from natural soils on an improved antibiotic medium]]></article-title>
<source><![CDATA[Nature.]]></source>
<year>1969</year>
<volume>223</volume>
<page-range>636-8</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
