<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1665-3521</journal-id>
<journal-title><![CDATA[Superficies y vacío]]></journal-title>
<abbrev-journal-title><![CDATA[Superf. vacío]]></abbrev-journal-title>
<issn>1665-3521</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Ciencia y Tecnología de Superficies y Materiales A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1665-35212007000200001</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Sensores suaves en procesos biotecnológicos]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cabrera Llanos]]></surname>
<given-names><![CDATA[A. I.]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ramírez Sotelo]]></surname>
<given-names><![CDATA[M. G.]]></given-names>
</name>
<xref ref-type="aff" rid="A01"/>
</contrib>
</contrib-group>
<aff id="A01">
<institution><![CDATA[,Instituto Politécnico Nacional Unidad Profesional Interdisciplinaria de Biotecnología ]]></institution>
<addr-line><![CDATA[México D. F.]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2007</year>
</pub-date>
<volume>20</volume>
<numero>2</numero>
<fpage>1</fpage>
<lpage>5</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1665-35212007000200001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1665-35212007000200001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1665-35212007000200001&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Se describe el desarrollo de un sensor virtual o suave aplicado a la estimación de las variables de un proceso de fermentación en lote alimentado para el cultivo de Sacharomyces cereviseae. Se desarrolla el algoritmo del estimador basado en redes neuronales diferenciales también llamado neuro-observador, se detallan los elementos que lo conforman y los resultados obtenidos al aplicarlo a una base de datos de dos experimentos de fermentación, con duración de 24 horas cada uno, finalmente se comparan la evolución temporal de las variables estimadas como la concentración de biomasa y la concentración de trehalosa en contra de las variables en una base de datos para determinar el desempeño del neuro-observador.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[In this paper we describe the virtual sensor or soft sensor applied to the fed batch fermentation process estimation with Sacharomyces cerevisiae yeast culture. The algorithm based on the differential neural networks is developed and this algorithm is called neuro-observer. The details for this structure are described and the application on the data for two fermentation process during a period of 24 hours of evolution of each one. Finally the estimate variables (biomass concentration and trehalose concentration) are compared versus the measured variables evolution to show the neuro-observer performance.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Sensor suave]]></kwd>
<kwd lng="es"><![CDATA[Redes neuronales diferenciales]]></kwd>
<kwd lng="es"><![CDATA[Procesos de Fermentación en lote alimentado]]></kwd>
<kwd lng="es"><![CDATA[Sacharomyces cereviseae]]></kwd>
<kwd lng="en"><![CDATA[Soft sensor]]></kwd>
<kwd lng="en"><![CDATA[Differential neural networks]]></kwd>
<kwd lng="en"><![CDATA[Fed batch fermentation process]]></kwd>
<kwd lng="en"><![CDATA[Sacharomyces cereviseae]]></kwd>
</kwd-group>
</article-meta>
</front><body><![CDATA[  	    <p align="center"><font face="verdana" size="4"><b>Sensores suaves en procesos biotecnol&oacute;gicos</b></font></p>  	    <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    <p align="center"><font face="verdana" size="2"><b>A. I. Cabrera Llanos, M. G. Ram&iacute;rez Sotelo</b></font></p>  	    <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    <p align="justify"><font face="verdana" size="2"><i>Unidad Profesional Interdisciplinaria de Biotecnolog&iacute;a del IPN, Barrio La Laguna Ticoman s/n, GAM, C. P. 07340 M&eacute;xico D. F.</i></font></p>  	    <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    <p align="justify"><font face="verdana" size="2">Recibido: 18 de marzo de 2007.    <br> 	Aceptado: 9 de mayo de 2007.</font></p>  	    <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    ]]></body>
<body><![CDATA[<p align="justify"><font face="verdana" size="2"><b>Resumen</b></font></p>  	    <p align="justify"><font face="verdana" size="2">Se describe el desarrollo de un sensor virtual o suave aplicado a la estimaci&oacute;n de las variables de un proceso de fermentaci&oacute;n en lote alimentado para el cultivo de <i>Sacharomyces cereviseae.</i> Se desarrolla el algoritmo del estimador basado en redes neuronales diferenciales tambi&eacute;n llamado neuro&#45;observador, se detallan los elementos que lo conforman y los resultados obtenidos al aplicarlo a una base de datos de dos experimentos de fermentaci&oacute;n, con duraci&oacute;n de 24 horas cada uno, finalmente se comparan la evoluci&oacute;n temporal de las variables estimadas como la concentraci&oacute;n de biomasa y la concentraci&oacute;n de trehalosa en contra de las variables en una base de datos para determinar el desempe&ntilde;o del neuro&#45;observador.</font></p>  	    <p align="justify"><font face="verdana" size="2"><b>Palabras clave:</b> Sensor suave; Redes neuronales diferenciales; Procesos de Fermentaci&oacute;n en lote alimentado; Sacharomyces cereviseae.</font></p>  	    <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    <p align="justify"><font face="verdana" size="2"><b>Abstract</b></font></p>  	    <p align="justify"><font face="verdana" size="2">In this paper we describe the virtual sensor or soft sensor applied to the fed batch fermentation process estimation with <i>Sacharomyces cerevisiae</i> yeast culture. The algorithm based on the differential neural networks is developed and this algorithm is called neuro&#45;observer. The details for this structure are described and the application on the data for two fermentation process during a period of 24 hours of evolution of each one. Finally the estimate variables (biomass concentration and trehalose concentration) are compared versus the measured variables evolution to show the neuro&#45;observer performance.</font></p>  	    <p align="justify"><font face="verdana" size="2"><b>Keywords:</b> Soft sensor; Differential neural networks; Fed batch fermentation process; Sacharomyces cereviseae.</font></p>  	    <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    <p align="justify"><font face="verdana" size="2"><a href="/pdf/sv/v20n2/v20n2a1.pdf" target="_blank">DESCARGAR ART&Iacute;CULO EN FORMATO PDF</a></font></p>     <p align="justify"><font face="verdana" size="2">&nbsp;</font></p>  	    ]]></body>
<body><![CDATA[<p align="justify"><font face="verdana" size="2"><b>Referencias</b></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;1&#93;&nbsp;J. de A Adilson, R. M. F., Soft sensors development for on&#45;line bioreactor state estimation, Computers and Chemical Engineering, 24, 1O99 (2OOO).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684509&pid=S1665-3521200700020000100001&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;2&#93; L. Fortuna, S. Grazini, Soft sensors for monitoring and control of industrial processes, 1st ed. (New York, Springer, 2006).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684511&pid=S1665-3521200700020000100002&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;3&#93; A. Cabrera, A. Poznyak, T. Poznyak, J. Aranda, Identification of a fed batch fermentation process: comparison of computational and laboratory experiments, Bioprocess and Biosystems Engineering, 24, 319 (2002).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684513&pid=S1665-3521200700020000100003&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;4&#93; O. Sotomayor, S. W. P., Software sensor for on&#45;line estimation of the microbial activity in activated sludge systems, The instrumentation, systems, and automation society, 41, 127 (2001).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684515&pid=S1665-3521200700020000100004&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;5&#93; L. Hudson, Neural Network and artificial intelligence for biomedical engineering, (Wiley&#45;IEEE Press, 2000).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684517&pid=S1665-3521200700020000100005&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;6&#93; S. Haykin, Neural Networks: A comprehensive Foundation. New York, (New York, Cambridge University Press, 1994).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684519&pid=S1665-3521200700020000100006&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;7&#93; A. V. W. Ph. Bogaerts, Parameter identification for state estimation &#45; application to bioprocess software sensor, Chemical Engineering Science, <b>59</b>, 2465 (2004).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684521&pid=S1665-3521200700020000100007&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;8&#93; B. McNeil,. L. M. Harvey, Fermentation a Practical Approach. (New York, IRL Press, 1990).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684523&pid=S1665-3521200700020000100008&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;9&#93; N. G. McDuffie, Biorreactor Design. (Oxford,Butterworth&#45;Heinemann., 1991).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684525&pid=S1665-3521200700020000100009&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;10&#93; A. S. Poznyak, E.N. Sanchez and W. Yu, Dynamic Neural Networks for Nonlinear Control: Identification, State Estimation and Trajectory Tracking. 1 st ed. (New Jersey, World Scientific, 2001).    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684527&pid=S1665-3521200700020000100010&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --></font></p>  	    <!-- ref --><p align="justify"><font face="verdana" size="2">&#91;11&#93;&nbsp;J. S. Aranda, E. Salgado and P. Taillandier, Biochem. Eng. J., 17, 129 (2004)</font>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[&#160;<a href="javascript:void(0);" onclick="javascript: window.open('/scielo.php?script=sci_nlinks&ref=9684529&pid=S1665-3521200700020000100011&lng=','','width=640,height=500,resizable=yes,scrollbars=1,menubar=yes,');">Links</a>&#160;]<!-- end-ref --> ]]></body><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Adilson]]></surname>
<given-names><![CDATA[J. de A]]></given-names>
</name>
<name>
<surname><![CDATA[M. F.]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang="en"><![CDATA[Soft sensors development for on-line bioreactor state estimation]]></article-title>
<source><![CDATA[Computers and Chemical Engineering]]></source>
<year>2OOO</year>
<volume>24</volume>
<page-range>1O99</page-range></nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Fortuna]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
<name>
<surname><![CDATA[Grazini]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[Soft sensors for monitoring and control of industrial processes]]></source>
<year>2006</year>
<edition>1</edition>
<publisher-loc><![CDATA[New York ]]></publisher-loc>
<publisher-name><![CDATA[Springer]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cabrera]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Poznyak]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
<name>
<surname><![CDATA[Poznyak]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
<name>
<surname><![CDATA[Aranda]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang="en"><![CDATA[Identification of a fed batch fermentation process: comparison of computational and laboratory experiments]]></article-title>
<source><![CDATA[Bioprocess and Biosystems Engineering]]></source>
<year>2002</year>
<volume>24</volume>
<page-range>319</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sotomayor]]></surname>
<given-names><![CDATA[O.]]></given-names>
</name>
<name>
<surname><![CDATA[S. W. P.]]></surname>
</name>
</person-group>
<article-title xml:lang="en"><![CDATA[Software sensor for on-line estimation of the microbial activity in activated sludge systems]]></article-title>
<source><![CDATA[The instrumentation, systems, and automation society]]></source>
<year>2001</year>
<volume>41</volume>
<page-range>127</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hudson]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Neural Network and artificial intelligence for biomedical engineering]]></source>
<year>2000</year>
<publisher-name><![CDATA[WileyIEEE Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Haykin]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<source><![CDATA[Neural Networks: A comprehensive Foundation]]></source>
<year>1994</year>
<publisher-loc><![CDATA[New York^eNew York New York]]></publisher-loc>
<publisher-name><![CDATA[Cambridge University Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bogaerts]]></surname>
<given-names><![CDATA[A. V. W. Ph.]]></given-names>
</name>
</person-group>
<article-title xml:lang="en"><![CDATA[Parameter identification for state estimation - application to bioprocess software sensor]]></article-title>
<source><![CDATA[Chemical Engineering Science]]></source>
<year>2004</year>
<volume>59</volume>
<page-range>2465</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[McNeil]]></surname>
<given-names><![CDATA[B.]]></given-names>
</name>
<name>
<surname><![CDATA[Harvey]]></surname>
<given-names><![CDATA[L. M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Fermentation a Practical Approach]]></source>
<year>1990</year>
<publisher-loc><![CDATA[New York ]]></publisher-loc>
<publisher-name><![CDATA[IRL Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[McDuffie]]></surname>
<given-names><![CDATA[N. G.]]></given-names>
</name>
</person-group>
<source><![CDATA[Biorreactor Design]]></source>
<year>1991</year>
<publisher-loc><![CDATA[OxfordButterworth ]]></publisher-loc>
<publisher-name><![CDATA[Heinemann]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Poznyak]]></surname>
<given-names><![CDATA[A. S.]]></given-names>
</name>
<name>
<surname><![CDATA[Sanchez]]></surname>
<given-names><![CDATA[E.N.]]></given-names>
</name>
<name>
<surname><![CDATA[Yu]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<source><![CDATA[Dynamic Neural Networks for Nonlinear Control: Identification, State Estimation and Trajectory Tracking]]></source>
<year>2001</year>
<edition>1</edition>
<publisher-loc><![CDATA[New Jersey ]]></publisher-loc>
<publisher-name><![CDATA[World Scientific]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aranda]]></surname>
<given-names><![CDATA[J. S.]]></given-names>
</name>
<name>
<surname><![CDATA[Salgado]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Taillandier]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<source><![CDATA[Biochem. Eng. J.]]></source>
<year>2004</year>
<volume>17</volume>
<page-range>129</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
