Effect of mineral supplementation and type of starch on the production of prodigiosin from a culture of Serratia marcescens BS303
Efecto de la suplementación mineral y el tipo de almidón en la producción de prodigiosina a partir de un cultivo de Serratia marcescens BS303
L. Chávez-Castilla and O. Aguilar*
Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, México. * Corresponding author. E-mail: alex.aguilar@itesm.mx Tel. +52 (81) 8358-2000 ext. 4821.
]]> Received May 4, 2015;
Abstract
Prodigiosin is a secondary metabolite that has become attractive for industrial microbiology by the large number of potential applications, but limitations like high price related to production, recovery and purification are responsible of the low industrial application. The production of the red pigment prodigiosin in Serratia marcescens is a regulated mechanism that depends on several factors, such as the presence of specific metal ions and carbon sources, for that reason the effect of four minerals and three types of starch as carbon sources on the cultivation behavior of Serratia marcescens BS303 was studied. The role of minerals on prodigiosin content resulted in 1.8-fold increase on the metabolite production using a combination of copper sulfate and ammonium ferric citrate at 0.05 g/L and it is related with the ability of the strain to regulate metal toxicity. The use of hydroxypropylated modified starch as a carbon source caused an increase of 8.1-fold on prodigiosin content and a growth rate of 0.06 h-1 showing pigment adsorption and probably scaffold properties for bacterial growth, enhancing prodigiosin production by 8.t-fold. Prodigiosin content raise 570 mg/L with mineral supplementation and polartex instant addition.
Keywords: Prodigiosin, Serratia marcescens, 2k factorial method, culture media, mineral supplementation.
Resumen
La prodigiosina es un metabolito secundario que se ha vuelto atractivo para la microbiología industrial por el gran número de aplicaciones demostradas, pero los altos precios relacionados con la producción, recuperación y purificación son responsables de limitar la aplicación industrial. La producción de prodigiosina a partir de Serratia marcescens es un mecanismo regulado que depende de varios factores, tales como la presencia de iones metálicos y la fuente de carbono, por esa razón se estudió el efecto de cuatro minerales y tres tipos de almidón como fuente de carbono en la producción de prodigiosina. El papel de los minerales en el contenido de prodigiosina resultó en un aumento de 1.8 veces, usando una combinación de sulfato de cobre y citrato de amonio férrico en una concentración de 0.05 g/L y se relacionó con la capacidad de la cepa para regular la toxicidad de metales. El uso de almidón hidroxipropilado como fuente de carbono causó un incremento de 8.1 veces el contenido de prodigiosina y una tasa de crecimiento de 0.06 h-1, originado por una adsorción del pigmento y generación de un andamio para el crecimiento bacteriano, obteniendo hasta 570 mg/L de prodigiosina.
Palabras clave: Prodigiosina, Serratia marcescens, diseño 2k, medio mineral.
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Acknowledgements
The authors wish to acknowledge the financial support of the Bioprocess and Synthetic Biology Research Group of Tecnológico de Monterrey and CONACYT for the doctoral fellowship of Luis Rodolfo Chávez Castilla No. 483935.
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