A statistically motivated choice of process parameters for the improvement of canthaxanthin production by Dietzia maris NIT-D (Accession number: HM151403)
Elección de parámetros de proceso mediante análisis estadístico para mejorar la producción de cantaxantina usando Dietzia maris NIT-D (Número de acceso: HM151403)
J. De Neve1*, G. Goswami2, A. Dutta3,4, S. Chaudhuri2 and D. Dutta2
1 Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University. Coupure Links 653, 9000 Ghent, Belgium. Corresponding author. E-mail: JanR.DeNeve@UGent.be Tel. +32 (0) 9 264 59 38, Fax +32 (0) 9 264 62 20.
2 Department of Biotechnology, National Institute of Technology, Durgapur, Ml. G. Avenue, Durgapur 713209, West Bengal, India.
]]> 3 Faculteit Industriële Ingenieurswetenschappen, KU Leuven, Campus Leuven (@Groep T), Andreas Vesaliusstraat 13, B-3000 Leuven, Belgium.4 Departement Metaalkunde ein Toegepaste Matertaalkunde (MTM), KU Leuven, Kasteelpark Arenberg 44, B-3001 Heverlee-Leuven, Belgium.
Received: December 12, 2013.
Accepted: February 8, 2014.
Abstract
Dietzia maris NIT-D, a producer of canthaxanthin, is isolated during routine screening of pigment producing bacteria. The effects of process parameters, namely temperature, pH, shaker speed, percentage inoculum, medium volume, and concentration of glucose on the canthaxanthin production are studied by using response surface design methodology. The optimal conditions are temperature = 30°C, pH = 5.5), shaker speed = 125 rpm, inoculum = 1.9 %, volume = 50 mL, and glucose = 15 g L-1, resulting in a canthaxanthin production of 152 mg L-1, which is 25% higher than that of a recently reported study.
Keywords: Dietzia maris NIT-D, biomass, canthaxanthin, response surface methodology.
]]> Resumen
Dietzia maris NIT-D una bacteria productora de cantaxantina, fue aislada durante una selección de cepas. Mediante un diseño de superficie de respuesta fueron estudiados los efectos de los parámetros del proceso para la producción de este pigmento: temperatura, pH, velocidad de agitación, porcentaje de inóculo, volumen del medio de cultivo y concentración de glucosa. Las condiciones óptimas fueron 30°C, pH de 5.9, 125 rpm, 1.9 % de inóculo, 50 mL de medio de cultivo y 15 g L-1 de glucosa. Como resultado la producción de cantaxantina fue de 152 mg L-1, 25% más que en un reciente estudio.
Palabras clave: Dietzia maris NIT-D, biomasa, cantaxantina, metodología de superficie de respuesta.
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References
Asker D. and Ohta Y. (1999) Production of canthaxanthin by extremely halophilic bacteria. Journal of Bioscience and Bioengineering 88, 617-621 [ Links ]
Asker D. and Ohta Y. (2002) Production of canthaxanthin by Haloferax alexandrinus under non-aseptic conditions and a simple, rapid method for its extraction. Applied Microbiology and Biotechnology 58, 743-750 [ Links ]
Borzenkov IA, Milekhina EI, Gotoeva MT, Rozanova EP and Belyaev SS (2006) The properties of hydrocarbon-oxidizing bacteria isolated from the oilfields of Tatarstan, Western Siberia, and Vietnam. Microbiology 75, 66-72 [ Links ]
Calo P., de Miguel T., Sieiro C., Velazquez JB and Villa TG (1995) Ketocarotenoids in halobacteria: 3-hydroxy echinenone and trans-astaxanthin. Journal of Applied Microbiology 79, 282-285 [ Links ]
Cooney JJ and Berry RA (1981) Inhibition of carotenoid synthesis in Micrococcus roseus. Canadian Journal of Microbiology 27,421-125 [ Links ]
Corona-González, RI, Ramos-Ibarra, JR, Gutiérrez-González, P, Pelayo-Ortiz, C, Guatemala-Morales, GM and Arriola-Guevara, E. (2013) The use of response surface methodology to evaluate the fermentation conditions in the production of tepache. Revista Mexicana de Ingeniería Química 12, 19-28 [ Links ]
Das A, Yoon S, Lee S, Kim J, Oh D and Kim S (2007) An update on microbial carotenoid production: application of recent metabolic engineering tools. Applied Microbiology and Biotechnology 77, 505-512 [ Links ]
de Miguel T, Sieiro C, Poza M and Villa TG (2000) Isolation and taxonomic study of a new canthaxanthin-containing bacterium, Gordonia jacobaea MV-1 sp. nov. International Microbiology 3, 107-111 [ Links ]
Don MM and Shoparwe NF (2010) Kinetics of hyarulonic acid production by Streptococcus zooepidemicus considering the effect of glucose. Biochemical Engineering Journal 49, 95-103 [ Links ]
Duckworth AW, Grant S, Grant WD, Jones BE and Meijer D (1998) Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake. Extremophiles 2, 359-366 [ Links ]
Gharibzahedi SMT, Razavi SH and Mousavi M (2013) Kinetic analysis and mathematical modelling of cell growth and canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1 on waste molasses hydrolysate. RSC Advances 3, 23495-23502 [ Links ]
Goswami G, Chakraborty S, Chaudhuri S and Dutta D (2012) Optimization of process parameters by response surface methodology and kinetic modeling for batch production of canthaxanthin by Dietzia maris NIT-D (accession number: M151403). Bioprocess and Biosystems Engineering 35, 1375-1388 [ Links ]
Hannibal L, Lorquin J, D'Ortoli NA, García N, Chaintreuil C, Masson-Boivin C, Dreyfus B and Giraud E (2000) Isolation and characterization of canthaxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. Strain ORS278. Journal of Bacteriology 182, 3850-3853 [ Links ]
Khodaiyan F, Razavi SH, Emam-Djomeh Z and Mousavi SM (2007a) Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 using response surface methodology. Pakistan Journal of Biological Sciences 10, 2544-2552 [ Links ]
Khodaiyan F, Razavi SH, Emam-Djomeh Z, Mousavi SM and Hejazi MA (2007b) Effect of culture conditions on canthaxanthin production by Dietzia natronolimnaea HS-1. Journal of Microbiology and Biotechnology 17, 195-201 [ Links ]
Khodaiyan F, Razavi SH, Mousavi SM (2008) Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 from cheese whey using statistical experimental methods. Biochemical Engineering Journal 40, 415-422 [ Links ]
Krupa D, Nakkeeran E, Kumaresan N, Vijayalakshmi G and Subramanian R (2010) Extraction, purification and concentration of partially saturated canthaxanthin from Aspergillus carbonarius. Bioresource Technology 101, 7598-7604 [ Links ]
Kutner MH, Nachtsheim CJ, Neter J and Li W (2005) Applied Linear Statistical Models, 5th edn. McGraw-Hill, New York [ Links ]
Lorquin J, Molouba F and Dreyfus BL (1997) Identification of the carotenoid pigment canthaxanthin from photosynthetic Bradyrhizobium strains. Applied and Environmental Microbiology 63, 1151-1154 [ Links ]
Malis, SA, Cohen E and Ben Amotz A (1993) Accumulation of canthaxanthin in Chlorella emersonii. Physiologia Plantarum 87, 232-236 [ Links ]
Moller AP, Biard C, Blount JD, Houston DC, Ninni P, Saino N and Surai PF (2000) Carotenoid- dependent signals: indicators of foraging efficiency, immunocompetence or detoxification ability? Avian and Poultry Biology Reviews 11, 137-159 [ Links ]
Montgomery DC (2001) Design and Analysis of Experiments, 5th edn. Wiley, New York [ Links ]
Nelis JH and de Leenheer AP (1989) Reinvestigation of Brevibacterium sp. Strain KY-4313 as a source of canthaxanthin. Applied and Environmental Microbiology 55, 2505-2510 [ Links ]
Padmapriya K, Dutta A, Chaudhuri S and Dutta D. (2012) Microwave assisted extraction of mangiferin from Curcuma amada. 3 Biotech 2, 27-30 [ Links ]
R Core Team (2012) R: A Language and Environment for Statistical Computing (http://www.R-project.org), Vienna, Austria [ Links ]
Rodriguez-Marín ML, Bello-Perez LA, Yee-Madeira H and González-Soto RA (2013) Propiedades mecánicas y de barrera de películas elaboradas con harina de arroz y plátano reforzadas con nanopartículas: estudio con superficie de respuesta. Revista Mexicana de Ingeniería Química 12, 165-176 [ Links ]
Veiga-Crespo P, Blasco L, Rosa dos Santos F, Poza M and Villa TG (2005) Influence of culture conditions of Gordonia jacobaea MV-26 on canthaxanthin production. International Microbiology 8, 55-58 [ Links ] ]]>