<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1405-3195</journal-id>
<journal-title><![CDATA[Agrociencia]]></journal-title>
<abbrev-journal-title><![CDATA[Agrociencia]]></abbrev-journal-title>
<issn>1405-3195</issn>
<publisher>
<publisher-name><![CDATA[Colegio de Postgraduados]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1405-31952007000700763</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Ocurrencia de un tobamovirus asociado con manchas anulares amarillas en nopal tunero en México]]></article-title>
<article-title xml:lang="en"><![CDATA[Occurrence of a tobamovirus associated with yellow ringspots in prickly pear cactus in Mexico]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Torre-Almaraz]]></surname>
<given-names><![CDATA[Rodolfo De La]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Salazar-Segura]]></surname>
<given-names><![CDATA[Mario]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ruiz-Medrano]]></surname>
<given-names><![CDATA[Roberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,UNAM FES-IZTACALA Unidad de Biotecnología y prototipos (UBIPRO)]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma Chapingo  ]]></institution>
<addr-line><![CDATA[Chapingo Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Politécnico Nacional Centro de Investigaciones Avanzadas CINVESTAV]]></institution>
<addr-line><![CDATA[ Distrito Federal]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>11</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>11</month>
<year>2007</year>
</pub-date>
<volume>41</volume>
<numero>7</numero>
<fpage>763</fpage>
<lpage>773</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S1405-31952007000700763&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S1405-31952007000700763&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S1405-31952007000700763&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: Se detectó un virus en cladodios de nopal tunero cultivado en la región de San Martín de las Pirámides, Estado de México, con manchas anulares de color amarillo. Los cladodios afectados desarrollan, en ataques severos, clorosis y marchitez. El virus se transmitió mecánicamente a algunas especies de plantas hospedantes indicadoras, causando lesiones locales cloróticas y después necróticas en las hojas inoculadas; sólo Nicotiana tabacum L. var. White Burley y Capsicum annuum L. desarrollaron además mosaicos tenues en hojas no inoculadas. El desarrollo de síntomas en los hospedantes alternos fue lento, pero se aceleraron a temperaturas mayores a 30 °C en invernadero. El análisis electroforético y el tratamiento con nucleasas del ARN replicativo mostró que el virus aislado de plantas de nopal tiene un genoma de ARN de cadena sencilla, mientras que el análisis al microscopio electrónico de macerados de tejidos de plantas de las especies de hospedantes indicadoras con mosaico, mostró partículas en forma de varilla rígida de 300-350 nm de longitud. Además, el ensayo por RT-PCR confirmó que el virus es una especie del grupo Tobamovirus. Las características biológicas de este virus indicaron que es una variante no severa relacionada con la especie Opuntia Sammons&#8217; virus.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: A virus was detected in fruit prickly pear cladodes cultivated in the region of San Martín de las Pirámides, State of Mexico, causing yellow ringspots. In severe attacks the affected cladodes develop chlorosis and wilting. The virus was transmitted mechanically to species of indicator host plants in which localized chlorotic lesions, later becoming necrotic, were caused on inoculated leaves; only Nicotiana tabacum L. var. White Burley and Capsicum annuum L. also developed tenuous mosaic on non-inoculated leaves. Symptom development on alternate hosts was slow, but accelerated at temperatures above 30 °C in a greenhouse. Electrophoretic analysis and treatment with double stranded RNA nuclease showed that the virus isolated from prickly pear plants has a single stranded RNA genome, while macerated tissue analysis of the indicator host species with mosaic under an electronic microscope showed rigid bar-shaped particles 300-350 mm long. Besides, an RT-PCR assay confirmed that the virus is a species of the Tobamovirus group. The biological characteristics of this virus indicate that it is a non-severe variant related to the species Opuntia Sammons&#8217; virus.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Opuntia amyclaea]]></kwd>
<kwd lng="es"><![CDATA[virus]]></kwd>
<kwd lng="en"><![CDATA[Opuntia amyclaea]]></kwd>
<kwd lng="en"><![CDATA[virus]]></kwd>
</kwd-group>
</article-meta>
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<surname><![CDATA[Watson]]></surname>
<given-names><![CDATA[A. L.]]></given-names>
</name>
<name>
<surname><![CDATA[Zurcher]]></surname>
<given-names><![CDATA[J. E.]]></given-names>
</name>
</person-group>
<source><![CDATA[Plant Viruses Online: description and Lists from the VIDE database]]></source>
<year>1989</year>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
