<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-7380</journal-id>
<journal-title><![CDATA[Revista fitotecnia mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. fitotec. mex]]></abbrev-journal-title>
<issn>0187-7380</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitogenética A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-73802022000200251</article-id>
<article-id pub-id-type="doi">10.35196/rfm.2022.2.251</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Capacidad de bacterias endófitas diazotróficas aisladas de maguey para solubilizar P Y Fe]]></article-title>
<article-title xml:lang="en"><![CDATA[Capacity of endophytic diazotrophic bacteria isolated from maguey to solubilize P AND Fe]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Moreno-Gómez]]></surname>
<given-names><![CDATA[Blanca]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aldana-Hernández]]></surname>
<given-names><![CDATA[Erika]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Moya]]></surname>
<given-names><![CDATA[Edmundo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aguado-Santacruz]]></surname>
<given-names><![CDATA[Gerardo A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias Campo Experimental Bajío ]]></institution>
<addr-line><![CDATA[Celaya Guanajuato]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,BIOqualitum  ]]></institution>
<addr-line><![CDATA[Celaya Guanajuato]]></addr-line>
<country>México</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,aff3  ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2022</year>
</pub-date>
<volume>45</volume>
<numero>2</numero>
<fpage>251</fpage>
<lpage>259</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802022000200251&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-73802022000200251&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-73802022000200251&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[RESUMEN Las bacterias promotoras de crecimiento vegetal son habitantes naturales del suelo y del interior de los tejidos de las plantas y son capaces de promover el crecimiento y la productividad de los cultivos. Cuando estas bacterias son susceptibles de ser aisladas y multiplicadas en laboratorio poseen un alto potencial para ser comercializadas y usadas para mejorar el rendimiento y sanidad de los cultivos. En este estudio se llevó a cabo el aislamiento, a partir de maguey, de bacterias endófitas diazotróficas con capacidad para solubilizar fosfato y hierro. Se colectaron pencas de maguey de las variedades Púa Larga, Ayoteco, Manso, Carricillo y Chalqueño en el Rancho Magueyero San Isidro, al suroeste de Nanacamilpa de Mariano Arista, Tlaxcala, México. El aislamiento de las bacterias endófitas se efectuó en el medio selectivo ELMARC y la identificación molecular posterior se llevó a cabo mediante la amplificación de un fragmento de la subunidad ribosomal 16S. Una vez realizada la identificación de las bacterias mediante la comparación de las secuencias amplificadas contra la base de datos del NCBI, se realizó una evaluación de las capacidades de las bacterias para fijar nitrógeno y solubilizar fosfato (P) y hierro (Fe) con base en las técnicas ARA (Ensayo de Reducción de Acetileno), Vanadato-Molibdato y Sulfonato de Cromo Azurol (CAS), respectivamente. Las pruebas ARA comprobaron la naturaleza diazotrófica de las bacterias seleccionadas en el medio ELMARC. La realización de pruebas cualitativas CAS mostró que, de 18 cepas aisladas, 12 presentaron alta capacidad para producir sideróforos. Posteriormente, la identificación de la naturaleza química de estas moléculas y las pruebas bioquímicas cuantitativas realizadas permitieron constatar que las cepas de Bacillus mojavensis (Bamo) y Leclercia sp. (Lesp2), con valores de 0.669 y 0.637 mg mL-1, respectivamente, fueron las mayores productoras de sideróforos tipo catecolato a las 24 h, mientras que Erwinia tasmaniensis (Erta) y Enterobacter sp. (Ensp1) destacaron por ser las mejores solubilizadoras de fosfato, mostrando actividades respectivas de 56.48 y 49.51 mg L-1 a las 96 h.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[SUMMARY Plant growth promoting bacteria are natural inhabitants of the soil and the interior of plant tissues and are capable of promoting the growth and productivity of crops. When these bacteria can be isolated and multiplied in the laboratory, they have a high potential to be commercialized and used to improve the yield and health of crops. In this study, the isolation from maguey of endophytic diazotrophic bacteria with the capacity to solubilize phosphate and iron was carried out. Maguey leaves of the Púa Larga, Ayoteco, Manso, Carricillo and Chalqueño varieties were collected at the San Isidro maguey producer ranch, in the southwest of Nanacamilpa de Mariano Arista, Tlaxcala, Mexico. Isolation of endophytic bacteria was carried out using the ELMARC selective medium, and subsequent molecular identification was carried out by amplifying a fragment of the 16S ribosomal subunit. Once the identification of the bacteria was carried out by comparing the amplified sequences against the NCBI database, an evaluation of the capabilities of the bacteria to fix nitrogen and solubilize phosphate (P) and iron (Fe) was carried out based on the ARA (Acetylene Reduction Assay), Vanadate-Molybdate and Chromium Azurol Sulfonate (CAS) techniques, respectively. The ARA tests verified the diazotrophic nature of the selected bacteria in the ELMARC medium. The qualitative CAS tests showed that, of 18 isolated strains, 12 showed high capacity to produce siderophores. Afterwards, the identification of the chemical nature of these molecules and the quantitative biochemical tests carried out, made it possible to verify that the strains of Bacillus mojavensis (Bamo) and Leclercia sp. (Lesp2), with values of 0.669 and 0.637 mg mL-1, respectively, were the highest producers of catecholate-type siderophores at 24 h, while Erwinia tasmaniensis (Erta) and Enterobacter sp. (Ensp1), stood out for being the best phosphate solubilizers, achieving respective activities of 56.48 and 49.51 mg L-1 at 96 h.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Bacterias diazotróficas]]></kwd>
<kwd lng="es"><![CDATA[bacterias endófitas]]></kwd>
<kwd lng="es"><![CDATA[maguey]]></kwd>
<kwd lng="es"><![CDATA[sideróforos]]></kwd>
<kwd lng="es"><![CDATA[solubilización de fosfatos]]></kwd>
<kwd lng="en"><![CDATA[Diazotrophic bacteria]]></kwd>
<kwd lng="en"><![CDATA[endophytic bacteria]]></kwd>
<kwd lng="en"><![CDATA[maguey]]></kwd>
<kwd lng="en"><![CDATA[phosphate solubilization]]></kwd>
<kwd lng="en"><![CDATA[siderophores]]></kwd>
</kwd-group>
</article-meta>
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