<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0187-7380</journal-id>
<journal-title><![CDATA[Revista fitotecnia mexicana]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. fitotec. mex]]></abbrev-journal-title>
<issn>0187-7380</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitogenética A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0187-73802020000400433</article-id>
<article-id pub-id-type="doi">10.35196/rfm.2020.4.433</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Identificación de Xylella fastidiosa subsp. fastidiosa en plantas cultivadas y silvestres en parras, Coahuila, México]]></article-title>
<article-title xml:lang="en"><![CDATA[Identification of Xylella fastidiosa subsp. fastidiosa in cultivated and wild plants in parras, Coahuila, Mexico]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Moya-Hernández]]></surname>
<given-names><![CDATA[Sandra L.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández-Anguiano]]></surname>
<given-names><![CDATA[Ana M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López-Buenfil]]></surname>
<given-names><![CDATA[J. Abel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aranda-Ocampo]]></surname>
<given-names><![CDATA[Sergio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aguilar-Granados]]></surname>
<given-names><![CDATA[Andrés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Nacional de Referencia Fitosanitaria  ]]></institution>
<addr-line><![CDATA[Tecámac Estado de México]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[Texcoco Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2020</year>
</pub-date>
<volume>43</volume>
<numero>4</numero>
<fpage>433</fpage>
<lpage>442</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0187-73802020000400433&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0187-73802020000400433&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0187-73802020000400433&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen Xylella fastidiosa es una bacteria Gram negativa que infecta plantaciones de vid (Vitis vinifera L.) en el municipio de Parras, Coahuila, México. La presente investigación tuvo como objetivo identificar la subespecie de X. fastidiosa y determinar su asociación con diferentes especies de plantas cultivadas y silvestres en el municipio. Durante el verano y otoño de 2018 se obtuvieron, en dos predios del municipio de Parras, 83 muestras procedentes de árboles frutales (n = 36), incluyendo vid, árboles forestales (n = 4), plantas ornamentales (n = 2) y plantas silvestres (n = 41). Para la identificación de la bacteria se amplificaron por PCR secuencias parciales de los genes gyrB, HL y 16S rRNA, así como de los genes de mantenimiento (MLST) leuA, petC, malF, cysG, holC, nuoL y gltT, además del gen de adherencia a superficies pilU. La reconstrucción filogenética mostró que las secuencias amplificadas correspondieron a X. fastidiosa subsp. fastidiosa. El clado X. fastidiosa subsp. fastidiosa mostró que las secuencias concatenadas de los genes MLST se agruparon con las secuencias ST1, ST2 y ST3, y las del gen pilU con la secuencia ST1. Los resultados confirmaron la presencia de X. fastidiosa subsp. fastidiosa en las especies cultivadas de Carya sp., Cydonia sp., Ficus carica, Olea europaea y V. vinifera, y en las especies silvestres Celtis pallida, Baccharis sp., Phragmites sp. y Rubus sp. Sólo en Cydonia sp., O. europaea, Phragmites sp. y V. vinifera se registró síntoma de escaldadura en las hojas, característico de X. fastidiosa. Los resultados indican que en el municipio de Parras, Coahuila se presentan infecciones recurrentes que representan una fuente de inóculo de la bacteria para los viñedos, principal cultivo de la región. Este es el primer reporte de X. fastidiosa subsp. fastidiosa en Cydonia sp., la cual se propone como nuevo hospedante de la bacteria.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Summary Xylella fastidiosa is a Gram negative bacterium that infects vine plantations (Vitis vinifera L.) in the municipality of Parras, Coahuila, México. This research aimed to identify the subspecies of X. fastidiosa and determine its association with different species of cultivated and wild plants in the municipality. During Summer and Autumn 2018, 83 samples of fruit trees (n = 36), including vine, forest trees (n = 4), ornamental plants (n = 2) and wild plants (n = 41) were collected from two sites in the municipality of Parras. For bacterial identification, partial sequences of the gyrB, HL, and 16S rRNA genes were amplified by PCR, as well as the housekeeping genes (MLST) leuA, petC, malF, cysG, holC, nuoL and gltT, in addition to the pilU adherence to surface gene. Phylogenetic reconstruction showed that the amplified sequences corresponded to X. fastidiosa subsp. fastidiosa. Clade X. fastidiosa subsp. fastidiosa and that the concatenated sequences of the MLST genes were grouped with the sequences ST1, ST2 and ST3, and those of the pilU gene with ST1. Results confirmed the presence of X. fastidiosa subsp. fastidiosa in the cultivated species of Carya sp., Cydonia sp., Ficus carica, Olea europaea and V. vinifera, and in the wild species Celtis pallida, Baccharis sp., Phragmites sp. and Rubus sp. Only in Cydonia sp., O. europaea, Phragmites sp. and V. vinifera scalding symptom was registered on the leaves, a characteristic of X. fastidiosa. Results show the presence of recurrent infections in the municipality of Parras, Coahuila, that represent a source of inoculum of the bacteria for the vineyards, the main crop in the region. This is the first report of X. fastidiosa subsp. fastidiosa in Cydonia sp., which is proposed as a new host for the bacterium.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Cydonia]]></kwd>
<kwd lng="es"><![CDATA[Olea europaea]]></kwd>
<kwd lng="es"><![CDATA[Phragmites]]></kwd>
<kwd lng="es"><![CDATA[Xylella fastidiosa]]></kwd>
<kwd lng="es"><![CDATA[MLST]]></kwd>
<kwd lng="es"><![CDATA[PCR]]></kwd>
<kwd lng="en"><![CDATA[Cydonia]]></kwd>
<kwd lng="en"><![CDATA[Olea europaea]]></kwd>
<kwd lng="en"><![CDATA[Phragmites]]></kwd>
<kwd lng="en"><![CDATA[Xylella fastidios]]></kwd>
<kwd lng="en"><![CDATA[MLST]]></kwd>
<kwd lng="en"><![CDATA[PCR]]></kwd>
</kwd-group>
</article-meta>
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