<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092022000200008</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.2202-7</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Omics applications in plant-microorganism interactions: A view from plant genomics]]></article-title>
<article-title xml:lang="es"><![CDATA[Aplicaciones Ómicas en la interacción Planta-Microorganismos: Una visión desde la genómica vegetal]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Simpson]]></surname>
<given-names><![CDATA[June]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Avila de Dios]]></surname>
<given-names><![CDATA[Emmanuel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gómez Vargas]]></surname>
<given-names><![CDATA[Alan D.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pérez-López]]></surname>
<given-names><![CDATA[Arely V.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castro Mexicano]]></surname>
<given-names><![CDATA[Andrea]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Meléndez Barraza]]></surname>
<given-names><![CDATA[Yoselin]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández Soriano]]></surname>
<given-names><![CDATA[Laura]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gálvez Sandré]]></surname>
<given-names><![CDATA[Laura]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[López Rosas]]></surname>
<given-names><![CDATA[M. Erendira]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Medina Chávez]]></surname>
<given-names><![CDATA[Rocío]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gil-Vega]]></surname>
<given-names><![CDATA[Katia D.C.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro de Investigación y de Estudios Avanzados Departamento de Ingeniería Genética de Plantas ]]></institution>
<addr-line><![CDATA[Irapuato Guanajuato]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2022</year>
</pub-date>
<volume>40</volume>
<numero>2</numero>
<fpage>263</fpage>
<lpage>269</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092022000200008&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092022000200008&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092022000200008&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract. The advent of next generation sequencing has opened up the possibility to carry out detailed molecular genetic analysis on non-model species. Here we present strategies to carry out RNAseq analysis in Agave tequilana which for the first time have permitted the identification and characterization of genes involved in fructan metabolism and flowering time in Agave species. Based on in silico data, expression patterns for individual genes could be determined and later confirmed by qRT-PCR and cDNA´s of genes of interest were cloned and functionally characterized in heterologous systems such as Pichia pastoris and Arabidopsis thaliana. We also present data obtained by RNAseq analysis of the C. lindemuthianum-P. vulgaris pathosystem. Using strains of C. lindemuthianum expressing the green fluorescent protein, the infection process can easily be followed. RNAseq was carried out at different time points during the initial stages of infection by C. lindemuthianum on P. vulgaris cultivar BAT93 using virulent or non-virulent races of C. lindemuthianum (Race 1088 and Race 256 respectively). Differentially expressed genes specific to compatible and non-compatible reactions could therefore be identified.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen. El desarrollo de la secuenciación de nueva generación ha abierto la posibilidad de llevar a cabo análisis genéticos moleculares detallados en especies no-modelo. Aquí se presentan estrategias para llevar a cabo análisis de RNAseq en Agave tequilana que por primera vez han permitido la identificación y caracterización de genes involucrados en el metabolismo de fructanos y el tiempo de floración en especies de Agave. Basándose en datos in silico, los patrones de expresión de genes individuales fueron determinados y luego confirmados mediante qRT-PCR. Además, los cDNA´s correspondientes a genes de interés fueron clonados y caracterizados funcionalmente en sistemas heterólogos como Pichia pastoris y Arabidopsis thaliana. También se presentan datos obtenidos por análisis de RNAseq del patosistema C. lindemuthianum-P. vulgaris. Usando cepas de C. lindemuthianum que expresan la proteína fluorescente verde, es sencillo dar seguimiento al proceso de infección y se llevó a cabo RNAseq en diferentes etapas del proceso inicial de infección por C. lindemuthianum en P. vulgaris cultivar BAT93 usando razas virulentas o no virulentas de C. lindemuthianum (Raza 1088 y Raza 256 respectivamente). Por lo tanto, pudieron identificarse genes expresados diferencialmente y específicos para reacciones compatibles y no compatibles.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Agave]]></kwd>
<kwd lng="en"><![CDATA[RNA-seq]]></kwd>
<kwd lng="en"><![CDATA[cDNA cloning]]></kwd>
<kwd lng="en"><![CDATA[C. lindemuthianum/P.vulgaris]]></kwd>
<kwd lng="en"><![CDATA[infection process]]></kwd>
<kwd lng="en"><![CDATA[virulence/avirulence]]></kwd>
<kwd lng="es"><![CDATA[Agave]]></kwd>
<kwd lng="es"><![CDATA[ARN-seq]]></kwd>
<kwd lng="es"><![CDATA[clonación cADN]]></kwd>
<kwd lng="es"><![CDATA[C. lindemuthianum/P.vulgaris]]></kwd>
<kwd lng="es"><![CDATA[proceso de infección]]></kwd>
<kwd lng="es"><![CDATA[virulencia/avirulencia]]></kwd>
</kwd-group>
</article-meta>
</front><back>
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