<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>0185-3309</journal-id>
<journal-title><![CDATA[Revista mexicana de fitopatología]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. fitopatol]]></abbrev-journal-title>
<issn>0185-3309</issn>
<publisher>
<publisher-name><![CDATA[Sociedad Mexicana de Fitopatología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S0185-33092019000300002</article-id>
<article-id pub-id-type="doi">10.18781/r.mex.fit.1905-5</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Detección por PCR de Guignardia bidwellii, agente causal de la pudrición negra de la vid]]></article-title>
<article-title xml:lang="en"><![CDATA[PCR detection of Guignardia bidwellii, causal agent of grape black rot]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Moreno-Velázquez]]></surname>
<given-names><![CDATA[Magnolia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández-Ramos]]></surname>
<given-names><![CDATA[Lervin]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Preuss-Angeles]]></surname>
<given-names><![CDATA[Ana Karen]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ronces-Frutos]]></surname>
<given-names><![CDATA[Liliana Elizabeth]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Morales-González]]></surname>
<given-names><![CDATA[Israel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Carrillo-Ortiz]]></surname>
<given-names><![CDATA[Nayeli]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cárcamo-Rodríguez]]></surname>
<given-names><![CDATA[Antonio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Centro Nacional de Referencia Fitosanitaria  ]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<volume>37</volume>
<numero>3</numero>
<fpage>383</fpage>
<lpage>398</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S0185-33092019000300002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S0185-33092019000300002&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S0185-33092019000300002&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen. Guignardia bidwellii (anamorfo: Phyllosticta ampelicida) es el agente etiológico de la pudrición negra de la vid, enfermedad de importancia económica en Europa y cuarentenaria para México. La identificación del estado anamorfo mediante caracteres morfológicos resulta complicada debido a la similitud entre especies del mismo género, por ejemplo, con el endófito cosmopolita G. endophyllicola (P. capitalensis), por lo que es necesario contar con herramientas de diagnóstico rápidas y específicas. Con este propósito se desarrolló un marcador molecular basado en los polimorfismos de un solo nucleótido (SNP) en la región ITS del ADNr, donde se diseñaron los oligos Bidwell y Ampel2 que mediante amplificación PCR generan un fragmento de 173 pb específico a G. bidwellii f. euvitis. La validación del método demostró que no hay amplificación cruzada con otros hongos fitopatógenos ni con el genoma de la vid. La técnica fue sensible al detectar hasta 30 pg µL-1 de ADN a partir de cultivos monospóricos y en mezcla con tejido vegetal. Se presenta este procedimiento de diagnóstico como una opción rápida y específica para el monitoreo y detección de G. bidwellii f. euvitis en apoyo a las estrategias de prevención, manejo, cuarentena y erradicación de la enfermedad.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract. Guignardia bidwellii (anamorph Phyllosticta ampelicida) is the etiological agent of grape black rot, a disease of economic importance in Europe and a quarantine pest for Mexico. The identification of the anamorphic state by morphological characteristics is complicated, due to the similarity among species of the same genus, for example with the cosmopolitan endophyte G. endophyllicola (P. capitalensis), therefore it is necessary to have quick and specific diagnostic tools. For this purpose, a molecular marker based on single nucleotide polymorphisms (SNP) in the ITS region of the rDNA was developed, where the Bidwell and Ampel2 primers were designed. The PCR amplification generates a fragment of 173 bp, specific to G. bidwellii f. euvitis. The validation of the method showed that there is no cross amplification with closely related phytopathogenic fungi or with the genome of grapevine. The technique was sensitive to detect up to 30 pg &#956;L-1 of DNA from monosporic cultures and in mixture of plant tissue. This diagnostic procedure is presented as a fast and specific option for the monitoring, and detection of G. bidwellii f. euvitis in support of prevention, management, quarantine and eradication strategies of the disease.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Phyllosticta ampelicida¸ diagnóstico molecular]]></kwd>
<kwd lng="es"><![CDATA[ITS]]></kwd>
<kwd lng="es"><![CDATA[Vitis spp]]></kwd>
<kwd lng="en"><![CDATA[Phyllosticta ampelicida]]></kwd>
<kwd lng="en"><![CDATA[molecular diagnosis]]></kwd>
<kwd lng="en"><![CDATA[ITS]]></kwd>
<kwd lng="en"><![CDATA[Vitis spp]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Altschul]]></surname>
<given-names><![CDATA[FS]]></given-names>
</name>
<name>
<surname><![CDATA[Gish]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
<name>
<surname><![CDATA[Miller]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
<name>
<surname><![CDATA[Myers]]></surname>
<given-names><![CDATA[WE]]></given-names>
</name>
<name>
<surname><![CDATA[Lipman]]></surname>
<given-names><![CDATA[JD.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Basic local alignment search tool]]></article-title>
<source><![CDATA[Journal of Molecular Biology]]></source>
<year>1990</year>
<volume>215</volume>
<page-range>403-10</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ayyadevara]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Thaden]]></surname>
<given-names><![CDATA[JJ]]></given-names>
</name>
<name>
<surname><![CDATA[Shmookler]]></surname>
<given-names><![CDATA[RJ.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Discrimination of primer 3&#8242;-nucleotide mismatch by Taq DNA Polymerase during Polymerase Chain Reaction]]></article-title>
<source><![CDATA[Analytical Biochemistry]]></source>
<year>2000</year>
<volume>284</volume>
<page-range>11-8</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Peter]]></surname>
<given-names><![CDATA[Bonants JM]]></given-names>
</name>
<name>
<surname><![CDATA[Carroll]]></surname>
<given-names><![CDATA[CG]]></given-names>
</name>
<name>
<surname><![CDATA[Weerdt]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Brouwershaven]]></surname>
<given-names><![CDATA[RI]]></given-names>
</name>
<name>
<surname><![CDATA[Baayen]]></surname>
<given-names><![CDATA[PR]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development and validation of a fast PCR-based detection method for pathogenic isolates of the citrus black spot fungus, Guignardia citricarpa]]></article-title>
<source><![CDATA[European Journal of Plant Pathology]]></source>
<year>2003</year>
<volume>109</volume>
<page-range>503-13</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cenis]]></surname>
<given-names><![CDATA[LJ]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Rapid extraction of fungal DNA for PCR amplification]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>1992</year>
<volume>20</volume>
<numero>9</numero>
<issue>9</issue>
<page-range>2380</page-range></nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Crous]]></surname>
<given-names><![CDATA[PW]]></given-names>
</name>
<name>
<surname><![CDATA[Verkleij]]></surname>
<given-names><![CDATA[GJM]]></given-names>
</name>
<name>
<surname><![CDATA[Groenewald]]></surname>
<given-names><![CDATA[JZ]]></given-names>
</name>
<name>
<surname><![CDATA[J]]></surname>
<given-names><![CDATA[Houbraken]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Laboratory Manual Series No. 1: Fungal Biodiversity]]></article-title>
<source><![CDATA[CBS Fungal Biodiversity Centre]]></source>
<year>2005</year>
<page-range>425</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Doyle]]></surname>
<given-names><![CDATA[JJ]]></given-names>
</name>
<name>
<surname><![CDATA[Doyle]]></surname>
<given-names><![CDATA[JL.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A rapid DNA isolation procedure for small quantities of fresh leaf tissue]]></article-title>
<source><![CDATA[Phytochemical Bulletin]]></source>
<year>1987</year>
<volume>19</volume>
<page-range>11-5</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Everett]]></surname>
<given-names><![CDATA[RK]]></given-names>
</name>
<name>
<surname><![CDATA[George Rees]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Species-specific PCR primers for Guignardia citricarpa and Guignardia mangiferae]]></article-title>
<source><![CDATA[New Zealand Plant Protection]]></source>
<year>2006</year>
<volume>59</volume>
<page-range>141-5</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hall]]></surname>
<given-names><![CDATA[TA.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT]]></article-title>
<source><![CDATA[Nucleic Acids Symposium Series]]></source>
<year>1999</year>
<volume>41</volume>
<page-range>95-8</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Humber]]></surname>
<given-names><![CDATA[AR]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fungi: Preservation of cultures]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Lawrence]]></surname>
<given-names><![CDATA[A. Lacey]]></given-names>
</name>
</person-group>
<source><![CDATA[Manual of techniques in insect pathology]]></source>
<year>1997</year>
<page-range>269-79</page-range><publisher-name><![CDATA[Academic Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hyun]]></surname>
<given-names><![CDATA[JW]]></given-names>
</name>
<name>
<surname><![CDATA[Peres]]></surname>
<given-names><![CDATA[NA]]></given-names>
</name>
<name>
<surname><![CDATA[Yi]]></surname>
<given-names><![CDATA[SY]]></given-names>
</name>
<name>
<surname><![CDATA[Timmer]]></surname>
<given-names><![CDATA[LW]]></given-names>
</name>
<name>
<surname><![CDATA[Kim]]></surname>
<given-names><![CDATA[KS]]></given-names>
</name>
<name>
<surname><![CDATA[Kwon]]></surname>
<given-names><![CDATA[HM]]></given-names>
</name>
<name>
<surname><![CDATA[Lim]]></surname>
<given-names><![CDATA[HC.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development of PCR assays for the identification of species and pathotypes of Elsinoë causing scab on citrus]]></article-title>
<source><![CDATA[Plant Disease]]></source>
<year>2007</year>
<volume>91</volume>
<page-range>865-70</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Luttrell]]></surname>
<given-names><![CDATA[SE.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Black rot of muscadine grapes]]></article-title>
<source><![CDATA[Phytopathology]]></source>
<year>1946</year>
<volume>36</volume>
<page-range>905-24</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Manchester]]></surname>
<given-names><![CDATA[KL]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Value of A260/A280 ratios for measurement of purity of nucleic acids]]></article-title>
<source><![CDATA[BioTechniques]]></source>
<year>1995</year>
<volume>19</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>208-10</page-range></nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Matveeva]]></surname>
<given-names><![CDATA[VO]]></given-names>
</name>
<name>
<surname><![CDATA[Shabalina]]></surname>
<given-names><![CDATA[AS]]></given-names>
</name>
<name>
<surname><![CDATA[Nemtsov]]></surname>
<given-names><![CDATA[AV]]></given-names>
</name>
<name>
<surname><![CDATA[Tsodikov]]></surname>
<given-names><![CDATA[DA]]></given-names>
</name>
<name>
<surname><![CDATA[Gesteland]]></surname>
<given-names><![CDATA[RF]]></given-names>
</name>
<name>
<surname><![CDATA[Atkins]]></surname>
<given-names><![CDATA[FJ]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Thermodynamic calculations and statistical correlations for oligo-probes design]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>2003</year>
<volume>31</volume>
<numero>14</numero>
<issue>14</issue>
<page-range>4211-7</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Keiichi]]></surname>
<given-names><![CDATA[Motohashi]]></given-names>
</name>
<name>
<surname><![CDATA[Inaba]]></surname>
<given-names><![CDATA[Shigeki]]></given-names>
</name>
<name>
<surname><![CDATA[Anzai]]></surname>
<given-names><![CDATA[Kozue]]></given-names>
</name>
<name>
<surname><![CDATA[Takamatsu]]></surname>
<given-names><![CDATA[Susumu]]></given-names>
</name>
<name>
<surname><![CDATA[Nakashima]]></surname>
<given-names><![CDATA[Chiharu]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phylogenetic analyses of Japanese species of Phyllosticta sensu stricto]]></article-title>
<source><![CDATA[Mycoscience]]></source>
<year>2009</year>
<volume>50</volume>
<page-range>291-302</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Okane]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Lumyong]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Nakagiri]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Ito]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Extensive host range of an endophytic fungus, Guignardia endophyllicola (anamorph: Phyllosticta capitalensis).]]></article-title>
<source><![CDATA[Mycoscience]]></source>
<year>2003</year>
<volume>44</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>353-63</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Peres]]></surname>
<given-names><![CDATA[NA]]></given-names>
</name>
<name>
<surname><![CDATA[Harakava]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Carroll]]></surname>
<given-names><![CDATA[CG]]></given-names>
</name>
<name>
<surname><![CDATA[Adaskaveg]]></surname>
<given-names><![CDATA[EJ]]></given-names>
</name>
<name>
<surname><![CDATA[Timmer]]></surname>
<given-names><![CDATA[WL.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Comparison of molecular procedures for detection and identification of Guignardia citricarpa and G. mangiferae]]></article-title>
<source><![CDATA[Plant Disease]]></source>
<year>2007</year>
<volume>91</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>525-31</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rinaldi]]></surname>
<given-names><![CDATA[AP]]></given-names>
</name>
<name>
<surname><![CDATA[Paffetti]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Comparini]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Broggini]]></surname>
<given-names><![CDATA[ALG]]></given-names>
</name>
<name>
<surname><![CDATA[Gessler]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Mugnai]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Genetic variability of Phyllosticta ampelicida, the agent of black rot disease of grapevine]]></article-title>
<source><![CDATA[Phytopathology]]></source>
<year>2017</year>
<volume>107</volume>
<page-range>1406-16</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rychlik]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Selection of Primers for Polymerase Chain Reaction]]></article-title>
<source><![CDATA[Molecular Biotechnology]]></source>
<year>1995</year>
<volume>3</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>129-34</page-range></nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sambrook]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Russell]]></surname>
<given-names><![CDATA[DW]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular Cloning: A Laboratory Manual. 3ª ed]]></source>
<year>2001</year>
<page-range>2344</page-range><publisher-loc><![CDATA[Nueva York. USA ]]></publisher-loc>
<publisher-name><![CDATA[Ed Cold Spring Harbor Laboratory Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Schoch]]></surname>
<given-names><![CDATA[LC]]></given-names>
</name>
<name>
<surname><![CDATA[Seifert]]></surname>
<given-names><![CDATA[AK]]></given-names>
</name>
<name>
<surname><![CDATA[Huhndorf]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Robert]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Spouge]]></surname>
<given-names><![CDATA[LJ]]></given-names>
</name>
<name>
<surname><![CDATA[Levesque]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Chen]]></surname>
<given-names><![CDATA[W.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi]]></article-title>
<source><![CDATA[Proceedings of the National Academy of Sciences of the United State of America]]></source>
<year>2012</year>
<volume>109</volume>
<numero>16</numero>
<issue>16</issue>
<page-range>6241-6</page-range></nlm-citation>
</ref>
<ref id="B21">
<nlm-citation citation-type="journal">
<collab>SENASICA</collab>
<article-title xml:lang=""><![CDATA[Pudrición negra de la vid (Guignardia bidwellii-Phyllosticta ampelicida). Servicio Nacional de Sanidad, Inocuidad y Calidad Agroalimentaria. Dirección General de Sanidad Vegetal]]></article-title>
<source><![CDATA[Programa de Vigilancia Epidemiológica Fitosanitaria]]></source>
<year>2019</year>
<publisher-loc><![CDATA[México, D.F ]]></publisher-loc>
</nlm-citation>
</ref>
<ref id="B22">
<nlm-citation citation-type="journal">
<collab>SIAP</collab>
<article-title xml:lang=""><![CDATA[Servicio de Información Agroalimentaria y Pesquera]]></article-title>
<source><![CDATA[Anuario estadístico de la producción agrícola: producción de vid]]></source>
<year>2018</year>
</nlm-citation>
</ref>
<ref id="B23">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Stadhouders]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Paz]]></surname>
<given-names><![CDATA[DS]]></given-names>
</name>
<name>
<surname><![CDATA[Anber]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Voermans]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Mes]]></surname>
<given-names><![CDATA[MHT]]></given-names>
</name>
<name>
<surname><![CDATA[Schutten]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5&#8242; nuclease assay]]></article-title>
<source><![CDATA[Journal of Molecular Diagnostics]]></source>
<year>2010</year>
<volume>12</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>109-17</page-range></nlm-citation>
</ref>
<ref id="B24">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ullrich]]></surname>
<given-names><![CDATA[IC]]></given-names>
</name>
<name>
<surname><![CDATA[Kleespies]]></surname>
<given-names><![CDATA[GR]]></given-names>
</name>
<name>
<surname><![CDATA[Enders]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Koch]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biology of the black rot pathogen, Guignardia bidwellii, its development in susceptible leaves of grapevine Vitis vinifera]]></article-title>
<source><![CDATA[Journal für Kulturflanzen]]></source>
<year>2009</year>
<volume>61</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>82-90</page-range></nlm-citation>
</ref>
<ref id="B25">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Untergasser]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Cutcutache]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Koressaar]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Ye]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Faircloth]]></surname>
<given-names><![CDATA[BC]]></given-names>
</name>
<name>
<surname><![CDATA[Remm]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Rozen]]></surname>
<given-names><![CDATA[SG.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Primer3 - new capabilities and interfaces]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>2012</year>
<volume>40</volume>
<numero>15</numero>
<issue>15</issue>
</nlm-citation>
</ref>
<ref id="B26">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Van der Aa]]></surname>
<given-names><![CDATA[H.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Studies in Phyllosticta]]></article-title>
<source><![CDATA[Studies in Mycology]]></source>
<year>1973</year>
<volume>5</volume>
<page-range>1-110</page-range></nlm-citation>
</ref>
<ref id="B27">
<nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[White]]></surname>
<given-names><![CDATA[JT]]></given-names>
</name>
<name>
<surname><![CDATA[Bruns]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Lee]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Taylor]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics]]></article-title>
<source><![CDATA[PCR Protocols: a guide to methods and applications]]></source>
<year>1990</year>
<page-range>315-22</page-range></nlm-citation>
</ref>
<ref id="B28">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wicht]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Petrini]]></surname>
<given-names><![CDATA[O]]></given-names>
</name>
<name>
<surname><![CDATA[Jermini]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Gessler]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Lodovico]]></surname>
<given-names><![CDATA[BGA.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Molecular, proteomic and morphological characterization of the ascomycete Guignardia bidwellii, agent of grape black rot: a polyphasic approach to fungal identification]]></article-title>
<source><![CDATA[Mycologia]]></source>
<year>2012</year>
<volume>104</volume>
<numero>5</numero>
<issue>5</issue>
<page-range>1036-45</page-range></nlm-citation>
</ref>
<ref id="B29">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wicht]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Jermini]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Gessler]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Lodovico]]></surname>
<given-names><![CDATA[BGA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Microsatellite markers for population studies of the ascomycete Phyllosticta ampelicida, the pathogen causing grape black rot]]></article-title>
<source><![CDATA[Phytopathologia Mediterranea]]></source>
<year>2014</year>
<volume>53</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>470&#8722;479</page-range></nlm-citation>
</ref>
<ref id="B30">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wikee]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Lombard]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Nakashima]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Motohashi]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Chukeatirote]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Cheewangkoon]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[McKenzie]]></surname>
<given-names><![CDATA[EH]]></given-names>
</name>
<name>
<surname><![CDATA[Hyde]]></surname>
<given-names><![CDATA[KD]]></given-names>
</name>
<name>
<surname><![CDATA[Crous]]></surname>
<given-names><![CDATA[PW]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales)]]></article-title>
<source><![CDATA[Studies in Mycology]]></source>
<year>2013</year>
<volume>76</volume>
<page-range>1-29</page-range></nlm-citation>
</ref>
<ref id="B31">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Wikee]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Lombard]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Crous]]></surname>
<given-names><![CDATA[WP]]></given-names>
</name>
<name>
<surname><![CDATA[Nakashima]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Motohashi]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Chukeatirote]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Alias]]></surname>
<given-names><![CDATA[AS]]></given-names>
</name>
<name>
<surname><![CDATA[McKenzie]]></surname>
<given-names><![CDATA[HCE]]></given-names>
</name>
<name>
<surname><![CDATA[Hyde]]></surname>
<given-names><![CDATA[DK.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Phyllosticta capitalensis, a widespread endophyte of plants]]></article-title>
<source><![CDATA[Fungal Diversity]]></source>
<year>2013</year>
<volume>60</volume>
<page-range>91-105</page-range></nlm-citation>
</ref>
<ref id="B32">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jian]]></surname>
<given-names><![CDATA[Ye]]></given-names>
</name>
<name>
<surname><![CDATA[Coulouris]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
<name>
<surname><![CDATA[Zaretskaya]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Cutcutache]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Rozen]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Madden]]></surname>
<given-names><![CDATA[LT.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction]]></article-title>
<source><![CDATA[BMC Bioinformatics]]></source>
<year>2012</year>
<volume>13</volume>
<page-range>134</page-range></nlm-citation>
</ref>
<ref id="B33">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ke]]></surname>
<given-names><![CDATA[Zhang]]></given-names>
</name>
<name>
<surname><![CDATA[Zhang]]></surname>
<given-names><![CDATA[Ning]]></given-names>
</name>
<name>
<surname><![CDATA[Cai.]]></surname>
<given-names><![CDATA[Lei]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Typification and phylogenetic study of Phyllosticta ampelicida and P. vaccinii]]></article-title>
<source><![CDATA[Mycologia]]></source>
<year>2013</year>
<volume>105</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>1030-42</page-range></nlm-citation>
</ref>
<ref id="B34">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zuker]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[mFold web server for nucleic acid folding and hybridization prediction]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>2003</year>
<volume>31</volume>
<page-range>3406-15</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
