<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2448-6760</journal-id>
<journal-title><![CDATA[Veterinaria México OA]]></journal-title>
<abbrev-journal-title><![CDATA[Veterinaria México OA]]></abbrev-journal-title>
<issn>2448-6760</issn>
<publisher>
<publisher-name><![CDATA[Universidad Nacional Autónoma de México, Facultad de Medicina Veterinaria y Zootecnia]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2448-67602017000400001</article-id>
<article-id pub-id-type="doi">10.21753/vmoa.4.4.469</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Turral Ramírez]]></surname>
<given-names><![CDATA[Ma. Montserrat]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Córdova Ponce]]></surname>
<given-names><![CDATA[Rodolfo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[González Ruíz]]></surname>
<given-names><![CDATA[Cynthia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Castañeda Aceves]]></surname>
<given-names><![CDATA[Graciela]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Marín Flamand]]></surname>
<given-names><![CDATA[Ernesto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Garrido Fariña]]></surname>
<given-names><![CDATA[Germán]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ramírez Álvarez]]></surname>
<given-names><![CDATA[Hugo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional Autónoma de México Facultad de Estudios Superiores Cuautitlán Laboratorio de Virología, Genética y Biología Molecular]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Nacional Autónoma de México Facultad de Estudios Superiores Cuautitlán Laboratorio de Patología]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Nacional Autónoma de México Facultad de Estudios Superiores Cuautitlán Laboratorio de Análisis Clínicos]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad Nacional Autónoma de México Facultad de Estudios Superiores Cuautitlán Laboratorio de apoyo a Histología y Biología]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2017</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2017</year>
</pub-date>
<volume>4</volume>
<numero>4</numero>
<fpage>0</fpage>
<lpage>0</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2448-67602017000400001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2448-67602017000400001&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2448-67602017000400001&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: The illegal trafficking of exotic birds such as parrots is now the third most lucrative business in the world and has been a problem for several years. As a result of this trafficking, there has been an increase in the emergence of exotic diseases. Herpesvirus is a pathogen of psittacines that has not been identified in Mexico to date. Through the use of polymerase chain reaction (PCR) assays and pathological analyses, the present study demonstrates the presence of herpesvirus in endemic and exotic psittacines in Mexico. The study was based on 50 birds, with clinical signs of herpervirus infection, and 45 clinically healthy birds. DNA samples were obtained from proctodaeal cells of the healthy birds, collected through the use of an enema; additionally, the liver, kidneys, brain, large intestines, and lungs of five birds exhibiting clinical signs suggestive of herpesvirus infection, were collected and fixed in buffered formalin for histopathological examination. PCR assays revealed herpesvirus infection in 8 clinically healthy birds, including four exotic species, and one endemic species. On necropsy, four of the five birds with clinical signs of herpesvirus infection showed lesions caused by herpesvirus, and the presence of intranuclear inclusion bodies in various tissues. Thus, these results demonstrate that there is a high risk of herpesvirus infection in endemic Mexican psittacines, which could significantly impact the health of these populations.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Herpesvirus]]></kwd>
<kwd lng="en"><![CDATA[Psittacines]]></kwd>
<kwd lng="en"><![CDATA[Proctodeum Cells]]></kwd>
<kwd lng="en"><![CDATA[PCR]]></kwd>
<kwd lng="en"><![CDATA[Histopathology]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="">
<collab>International Committee on Taxonomy of Viruses ICTV</collab>
<source><![CDATA[Virus taxonomy]]></source>
<year>2016</year>
</nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[MacLachlan]]></surname>
<given-names><![CDATA[NJ]]></given-names>
</name>
<name>
<surname><![CDATA[Dubovi]]></surname>
<given-names><![CDATA[EJ]]></given-names>
</name>
</person-group>
<source><![CDATA[Fenner's veterinary virology]]></source>
<year>2011</year>
<edition>4</edition>
<publisher-loc><![CDATA[London(UK) ]]></publisher-loc>
<publisher-name><![CDATA[Elsevier Academic Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Norkin]]></surname>
<given-names><![CDATA[LC]]></given-names>
</name>
</person-group>
<source><![CDATA[Virology: Molecular biology and pathogenesis]]></source>
<year>2010</year>
<publisher-loc><![CDATA[Washington DC(US) ]]></publisher-loc>
<publisher-name><![CDATA[ASM Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Carrasco-Llamas]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Almendral]]></surname>
<given-names><![CDATA[JM]]></given-names>
</name>
<name>
<surname><![CDATA[Alcamí]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Carrascosa]]></surname>
<given-names><![CDATA[JL]]></given-names>
</name>
<name>
<surname><![CDATA[Carrascosa]]></surname>
<given-names><![CDATA[ÁL]]></given-names>
</name>
<name>
<surname><![CDATA[Enjuanes]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
</person-group>
<source><![CDATA[Virus patógenos]]></source>
<year>2006</year>
<publisher-loc><![CDATA[Madrid(SP) ]]></publisher-loc>
<publisher-name><![CDATA[Hélice y Fundación BBVA]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Tissue distribution of psittacid herpesviruses in latently infected parrots, repeated sampling of latently infected parrots and prevalence of latency in parrots submitted for necropsy]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tomaszewski]]></surname>
<given-names><![CDATA[EK]]></given-names>
</name>
<name>
<surname><![CDATA[Wigle]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
<name>
<surname><![CDATA[Phalen]]></surname>
<given-names><![CDATA[DN]]></given-names>
</name>
</person-group>
<source><![CDATA[J Vet Diagn Invest]]></source>
<year>2006</year>
<volume>18</volume>
<page-range>536-44</page-range></nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Pacheco's disease in a Hungarian zoo bird population a case report]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bistyák]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Kecskeméti]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Glávits]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Tischler]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Nagy]]></surname>
<given-names><![CDATA[ST]]></given-names>
</name>
<name>
<surname><![CDATA[Kardos]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<source><![CDATA[Acta Vet Hung]]></source>
<year>2007</year>
<volume>55</volume>
<page-range>213-8</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[A survey for selected viral, chlamydial, and parasitic diseases in wild dusky-headed parakeets (Aratinga weddellii) and tui parakeets (Brotogeris sanctithomae) in Peru]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gilardi]]></surname>
<given-names><![CDATA[KV]]></given-names>
</name>
<name>
<surname><![CDATA[Lowenstine]]></surname>
<given-names><![CDATA[LJ]]></given-names>
</name>
<name>
<surname><![CDATA[Gilardi]]></surname>
<given-names><![CDATA[JD]]></given-names>
</name>
<name>
<surname><![CDATA[Munn]]></surname>
<given-names><![CDATA[CA]]></given-names>
</name>
</person-group>
<source><![CDATA[J Wildl Dis]]></source>
<year>1995</year>
<volume>31</volume>
<page-range>523-8</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ritchie]]></surname>
<given-names><![CDATA[BW]]></given-names>
</name>
<name>
<surname><![CDATA[Harrison]]></surname>
<given-names><![CDATA[GJ]]></given-names>
</name>
<name>
<surname><![CDATA[Harrison]]></surname>
<given-names><![CDATA[LR]]></given-names>
</name>
</person-group>
<source><![CDATA[Avian medicine. Principles and application]]></source>
<year>1994</year>
<publisher-loc><![CDATA[Florida (US) ]]></publisher-loc>
<publisher-name><![CDATA[Wingers Publishing, Inc.]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Detection and heterogeneity of herpesviruses causing Pachecos´s disease in parrots]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tomaszewski]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Wilson]]></surname>
<given-names><![CDATA[VG]]></given-names>
</name>
<name>
<surname><![CDATA[Wigle]]></surname>
<given-names><![CDATA[WL]]></given-names>
</name>
<name>
<surname><![CDATA[Phalen]]></surname>
<given-names><![CDATA[DN]]></given-names>
</name>
</person-group>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2001</year>
<volume>39</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>533-8</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="">
<collab>Cambrico Biotech</collab>
<source><![CDATA[Enfermedad de Pacheco: Herpesvirus]]></source>
<year>2015</year>
</nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Molecular genetic and pathogenic characterization of psittacid herpesvirus type 1 isolated from a captive galah (Eolophus roseicapillus) in Japan]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Katoh]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Yamada]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Hagino]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Ohya]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Sakai]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Yanai]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<source><![CDATA[J Vet Med Sci]]></source>
<year>2011</year>
<volume>73</volume>
<numero>10</numero>
<issue>10</issue>
<page-range>1341-5</page-range></nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="">
<collab>Procuraduría Federal de Protección al Ambiente (PROFEPA)</collab>
<source><![CDATA[Tráfico ilegal de especies. Ciudad de México (MX): gob.mx]]></source>
<year>2010</year>
</nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[A novel herpesvirus associated with respiratory disease in Bourke´s parrots (Neopsephotus bourkii)]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Shivaprasad]]></surname>
<given-names><![CDATA[HL]]></given-names>
</name>
<name>
<surname><![CDATA[Phalen]]></surname>
<given-names><![CDATA[DN]]></given-names>
</name>
</person-group>
<source><![CDATA[Avian Pathol]]></source>
<year>2012</year>
<volume>41</volume>
<page-range>531-9</page-range></nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Molecular phylogeny of the psittacid herpesviruses causing Pacheco's disease correlation of genotype with phenotypic expression]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tomaszewski]]></surname>
<given-names><![CDATA[EK]]></given-names>
</name>
<name>
<surname><![CDATA[Kaleta]]></surname>
<given-names><![CDATA[EF]]></given-names>
</name>
<name>
<surname><![CDATA[Phalen]]></surname>
<given-names><![CDATA[DN]]></given-names>
</name>
</person-group>
<source><![CDATA[J Virol]]></source>
<year>2003</year>
<volume>77</volume>
<page-range>11260-7</page-range></nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[DNA from formalin-fixed tissue extraction or repair? That is the question]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Skage]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Schander]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
</person-group>
<source><![CDATA[Mar Biol Res]]></source>
<year>2007</year>
<volume>3</volume>
<page-range>289-95</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Comparison of protocols for DNA extraction from long-term preserved formalin fixed tissues]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Paireder]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Werner]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Bailer]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Werther]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
<name>
<surname><![CDATA[Schmid]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Patzak]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
</person-group>
<source><![CDATA[Anal Biochem]]></source>
<year>2013</year>
<volume>439</volume>
<page-range>152-60</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[A review of DNA viral infections in psittacine birds]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Katoh]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Ogawa]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Ohya]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Fukushi]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
</person-group>
<source><![CDATA[J Vet Med Sci]]></source>
<year>2010</year>
<volume>72</volume>
<page-range>1099-106</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Identification and isolation of psittacid herpesvirus from psittacids in Brazil]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Miranda-Luppi]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Luiz]]></surname>
<given-names><![CDATA[AP]]></given-names>
</name>
<name>
<surname><![CDATA[Coelho]]></surname>
<given-names><![CDATA[FM]]></given-names>
</name>
<name>
<surname><![CDATA[Malta]]></surname>
<given-names><![CDATA[MdC]]></given-names>
</name>
<name>
<surname><![CDATA[Preis]]></surname>
<given-names><![CDATA[IS]]></given-names>
</name>
<name>
<surname><![CDATA[Ecco]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
</person-group>
<source><![CDATA[Vet Microbiol]]></source>
<year>2011</year>
<volume>154</volume>
<numero>1-2</numero>
<issue>1-2</issue>
<page-range>69-77</page-range></nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<article-title xml:lang=""><![CDATA[Genetic characterization of a herpesvirus isolate from a superb starling (Lamprotornis superbus) as a psittacid herpesvirus genotype 1]]></article-title>
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tomaszewski]]></surname>
<given-names><![CDATA[EK]]></given-names>
</name>
<name>
<surname><![CDATA[Gravendyck]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Kaleta]]></surname>
<given-names><![CDATA[EF]]></given-names>
</name>
<name>
<surname><![CDATA[Phalen]]></surname>
<given-names><![CDATA[DN]]></given-names>
</name>
</person-group>
<source><![CDATA[Avian Dis]]></source>
<year>2004</year>
<volume>48</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>212-4</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
