<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2444-054X</journal-id>
<journal-title><![CDATA[Cirugía y cirujanos]]></journal-title>
<abbrev-journal-title><![CDATA[Cir. cir.]]></abbrev-journal-title>
<issn>2444-054X</issn>
<publisher>
<publisher-name><![CDATA[Academia Mexicana de Cirugía A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2444-054X2019000600619</article-id>
<article-id pub-id-type="doi">10.24875/ciru.19000705</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Efecto de la refrigeración en la viabilidad y la apoptosis de los adipocitos decantados después de la liposucción]]></article-title>
<article-title xml:lang="en"><![CDATA[Effect of refrigeration in the viability and apoptosis of decanted adipocytes after liposuction]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mecott]]></surname>
<given-names><![CDATA[Gabriel A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Chacón-Martínez]]></surname>
<given-names><![CDATA[Hernán]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Leal-Torres]]></surname>
<given-names><![CDATA[Oscar H.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Flores-García]]></surname>
<given-names><![CDATA[Pablo P.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Montes de Oca]]></surname>
<given-names><![CDATA[Roberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[García-Pérez]]></surname>
<given-names><![CDATA[Mauricio M.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma de Nuevo León Hospital Universitario &#8220;Dr. José Eleuterio González&#8221; Servicio de Cirugía Plastica y Reconstructiva]]></institution>
<addr-line><![CDATA[Monterrey ]]></addr-line>
<country>México</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma de Nuevo León Hospital Universitario &#8220;Dr. José Eleuterio González&#8221; Departamento de Histología]]></institution>
<addr-line><![CDATA[Monterrey ]]></addr-line>
<country>México</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Autónoma de Nuevo León Hospital Universitario &#8220;Dr. José Eleuterio González&#8221; Facultad de Medicina]]></institution>
<addr-line><![CDATA[Monterrey ]]></addr-line>
<country>México</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2019</year>
</pub-date>
<volume>87</volume>
<numero>6</numero>
<fpage>619</fpage>
<lpage>623</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2444-054X2019000600619&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2444-054X2019000600619&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2444-054X2019000600619&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Abstract  Objective: To evaluate the effect of refrigeration in the apoptosis and viability of the lipoaspirate in the first 2 h after harvesting.  Methods: 20 consecutive patients who underwent liposuction from the abdomen for esthetic reasons were included. 5 ml of fat were obtained and processed for study. The viability was obtained using trypan blue. Apoptosis was determined using TUNEL assay.  Results: All patients were female with a median age of 36.5 (21-67) years. On regard of the viability, at time 0, the viability in the control group was 59.08 ± 24% and 60.96 ± 22% in the refrigeration group. At 60 min, the values were 50.82 ± 21% versus 55 ± 32.6% (p = 0.74) and a 120 min, 42.69 ± 20.85% and 50.33 ± 21% respectively. On regard of apoptosis, the percentage of apoptotic cells at time 0 was 37.87 ± 9.7% for the control group and 34.28 ± 9.74% for refrigerated samples. At 60 min 51.11 ± 8.64% versus 45.94 ± 9.15% and at 120 min, 62.97 ± 13.33% versus 55.81 ± 9.45% respectively.  Conclusions: Refrigerating the lipoaspirate at 4 °C decreased the mortality and apoptosis of the adipocytes in &lt;10% within the first 2 h from harvesting.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Resumen  Objetivo: Evaluar el efecto de la refrigeración en la apoptosis y la viabilidad del lipoaspirado en las primeras 2 horas después de la toma.  Método: Se incluyeron 20 pacientes que fueron sometidas a una liposucción del abdomen por razones estéticas. Se obtuvieron 5 ml de grasa y se procesaron para su estudio. La viabilidad se determinó usando azul tripano. La apoptosis se determinó usando el ensayo TUNEL.  Resultados: Todas las pacientes eran mujeres, con una edad media de 36.5 años (rango: 21-67). Con respecto a la viabilidad, en el tiempo 0, en el grupo control fue del 59.08 ± 24% y en el grupo de refrigeración fue del 60.96 ± 22%; a los 60 minutos, los valores fueron del 50.82 ± 21% y el 55 ± 32.6%, respectivamente (p = 0.74); y a los 120 minutos, fueron del 42.69 ± 20.85% y el 50.33 ± 21%, respectivamente. En cuanto a la apoptosis, el porcentaje de células apoptóticas en el tiempo 0 fue del 37.87 ± 9.7% para el grupo de control y del 34.28 ± 9.74% para las muestras refrigeradas; a los 60 minutos, del 51.11 ± 8.64% y el 45.94 ± 9.15%, respectivamente; y a los 120 minutos, del 62.97 ± 13.33% y el 55.81 ± 9.45%, respectivamente.  Conclusiones: Refrigerar el lipoaspirado a 4 °C disminuyó la mortalidad y la apoptosis de los adipocitos en menos del 10% en las primeras 2 horas desde la toma.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Apoptosis]]></kwd>
<kwd lng="en"><![CDATA[Refrigeration]]></kwd>
<kwd lng="en"><![CDATA[Viability]]></kwd>
<kwd lng="en"><![CDATA[Adipocytes]]></kwd>
<kwd lng="en"><![CDATA[Liposuction]]></kwd>
<kwd lng="es"><![CDATA[Apoptosis]]></kwd>
<kwd lng="es"><![CDATA[Refrigeración]]></kwd>
<kwd lng="es"><![CDATA[Viabilidad]]></kwd>
<kwd lng="es"><![CDATA[Adipocitos]]></kwd>
<kwd lng="es"><![CDATA[Liposucción]]></kwd>
</kwd-group>
</article-meta>
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