<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-3364</journal-id>
<journal-title><![CDATA[Therya]]></journal-title>
<abbrev-journal-title><![CDATA[Therya]]></abbrev-journal-title>
<issn>2007-3364</issn>
<publisher>
<publisher-name><![CDATA[Asociación Mexicana de Mastozoología A.C.]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-33642024000100051</article-id>
<article-id pub-id-type="doi">10.12933/therya-24-5746</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Applicability of DNA barcoding-based analyses on the diet of the Gray brocket deer (Subulo gouazoubira) in xeric hillside forests]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cosse]]></surname>
<given-names><![CDATA[Mariana]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bruno]]></surname>
<given-names><![CDATA[Antonella]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mannise]]></surname>
<given-names><![CDATA[Natalia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bou]]></surname>
<given-names><![CDATA[Nadia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Bonifacino]]></surname>
<given-names><![CDATA[Mauricio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Camargo]]></surname>
<given-names><![CDATA[Arley]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Smircich]]></surname>
<given-names><![CDATA[Pablo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Iriarte]]></surname>
<given-names><![CDATA[Andrés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Brazeiro]]></surname>
<given-names><![CDATA[Alejandro]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Instituto de Investigaciones Biológicas Clemente Estable Departamento de Biodiversidad y Genética ]]></institution>
<addr-line><![CDATA[Montevideo ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad de la República Centro Universitario Regional del Este Sede Maldonado ]]></institution>
<addr-line><![CDATA[Maldonado ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad de la República Instituto de Ecología y Ciencias Ambientales, Facultad de Ciencias ]]></institution>
<addr-line><![CDATA[Montevideo ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Universidad de la República Facultad de Agronomía Laboratorio de Botánica]]></institution>
<addr-line><![CDATA[Montevideo ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af5">
<institution><![CDATA[,Universidad de la República Centro Universitario Regional Noreste, Sede Rivera Laboratorio de Biogeografía y Evolución]]></institution>
<addr-line><![CDATA[Rivera ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af6">
<institution><![CDATA[,Instituto de Investigaciones Biológicas Clemente Estable Departamento de Genómica Laboratorio de Bioinformática]]></institution>
<addr-line><![CDATA[Montevideo ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af7">
<institution><![CDATA[,Universidad de la República Facultad de Medicina Instituto de Higiene]]></institution>
<addr-line><![CDATA[Montevideo ]]></addr-line>
<country>Uruguay</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2024</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2024</year>
</pub-date>
<volume>15</volume>
<numero>1</numero>
<fpage>51</fpage>
<lpage>58</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-33642024000100051&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-33642024000100051&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-33642024000100051&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[In this study, we explore the applicability of DNA barcoding, specifically targeting the chloroplast DNA (cpDNA) sequences, particularly the trnL (UAA) intron region, to analyze the diet of gray brocket deer. This approach offers improved taxonomic resolution and the ability to identify species with greater precision compared to traditional methods. The study was conducted in the "Reserva Natural Salus" in Uruguay, covering a range of vegetation types, where gray brocket deer coexist with other exotic ungulates. A local reference database of trnL (UAA) sequences was established, incorporating both GenBank data and sequences obtained from native species in the study area. Fecal samples were collected in summer and winter, and DNA was extracted and amplified for metabarcoding analysis in pooled samples for each season. For each sample 28,229 and 33,588 reads were obtained respectively, which together corresponded to 25 Operational Taxonomic Units (OTUs). The species Rubus ulmifolius and Schinus engleri were the most represented in the diet with 69.6 % of the summer reads, whereas in winter, 68.7 % of the reads corresponded only to Schinus engleri. These findings indicate that gray brocket deer consume species that have higher nutritional value, which may be linked to their capacity to thrive in young and productive ecosystems. This study demonstrates the feasibility of DNA barcoding for dietary analysis in gray brocket deer and provides valuable insights into their food habits in the "Reserva Natural Salus". Further improvements to increase the reference databases of native species and the exploration of additional genetic markers are recommended for enhanced species-level discrimination in dietary analysis studies. This methodology is promising for future research as diet studies have an impact on species management, habitat conservation and biodiversity conservation efforts.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[En este estudio, exploramos la aplicabilidad de los códigos de barras de ADN, utilizando la región de intrón del cloroplasto trnL (UAA), para analizar la dieta del guazubirá. Este enfoque ofrece una buena resolución taxonómica y la capacidad de identificar especies con mayor precisión que con los métodos tradicionales basados en análisis microhistológicos de fragmentos vegetales. El estudio se realizó en la "Reserva Natural Salus" de Uruguay, que presenta distintos tipos de vegetación, incluyendo bosques nativos, plantaciones de pinos y eucaliptos, y afloramientos rocosos, donde el guazubirá convive con otros ungulados exóticos. Para el estudio se estableció una base de referencia local de secuencias trnL (UAA), incorporando registros de GenBank y secuencias obtenidas de especies nativas en el área de estudio. Se recolectaron muestras fecales en verano e invierno, se extrajo y amplificó el ADN para análisis de metabarcoding, agrupando las muestras colectadas en cada estación. Para cada uno de los muestreos se obtuvieron 28,229 y 33,588 lecturas respectivamente, que en conjunto representaron 25 Unidades Taxonómicas Operativas (UTOs). Sin embargo, las especies Rubus ulmifolius y Schinus engleri fueron las más representadas con el 69.6 % de las lecturas de verano, para invierno el 68.7 % de las lecturas fueron para Schinus engleri. Estos hallazgos indican que el guazubirá consume especies que tienen alto valor nutricional, lo cual podría relacionarse con la habilidad de esta especie a adaptarse a ecosistemas jóvenes y altamente productivos. Este estudio demuestra la viabilidad de estudios de dieta basados en códigos de barras de ADN para ciervos neotropicales y proporciona información valiosa sobre los hábitos alimentarios del guazubirá en la "Reserva Natural Salus". Se recomienda ampliar las bases de secuencias de referencia de especies nativas y explorar otros marcadores genéticos adicionales para mejorar el poder de discriminación a nivel de especie en los estudios de metabarcoding de especies vegetales. Esta metodología es prometedora para futuras investigaciones, ya que los estudios de dieta tienen un impacto en el manejo de especies, la conservación del hábitat y los esfuerzos de conservación de la biodiversidad.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[DNA metabarcoding]]></kwd>
<kwd lng="en"><![CDATA[food habits]]></kwd>
<kwd lng="en"><![CDATA[mammal]]></kwd>
<kwd lng="en"><![CDATA[neotropical cervids]]></kwd>
<kwd lng="en"><![CDATA[noninvasive]]></kwd>
<kwd lng="en"><![CDATA[species identification]]></kwd>
<kwd lng="en"><![CDATA[trnL (UAA)]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ahmad]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Antioxidant and nutraceutical value of wild medicinal Rubus berries]]></article-title>
<source><![CDATA[Pakistan Journal of Pharmaceutical Sciences]]></source>
<year>2015</year>
<volume>28</volume>
<page-range>241-7</page-range></nlm-citation>
</ref>
<ref id="B2">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Alberdi]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Promises and pitfalls of using high-throughput sequencing for diet analysis]]></article-title>
<source><![CDATA[Molecular Ecology Resources]]></source>
<year>2019</year>
<volume>19</volume>
<page-range>327-48</page-range></nlm-citation>
</ref>
<ref id="B3">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Altschul]]></surname>
<given-names><![CDATA[S. F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Gapped BLAST and PSI-BLAST: a new generation of protein database search programs]]></article-title>
<source><![CDATA[Nucleic acids research]]></source>
<year>1997</year>
<volume>25</volume>
<page-range>3389-402</page-range></nlm-citation>
</ref>
<ref id="B4">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aristimuño]]></surname>
<given-names><![CDATA[M. P.]]></given-names>
</name>
</person-group>
<source><![CDATA[Variabilidad genética en poblaciones de Mazama gouazoubira (Mammalia: Cervidae Fischer, 1814) de Uruguay y la región]]></source>
<year>2013</year>
<publisher-loc><![CDATA[Udelar ]]></publisher-loc>
<publisher-name><![CDATA[Grade, Facultad de Ciencias]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B5">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Benson]]></surname>
<given-names><![CDATA[D. A.]]></given-names>
</name>
</person-group>
<source><![CDATA[GenBank]]></source>
<year>2013</year>
<publisher-name><![CDATA[Nucleic Acids Res 2012/11/30]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B6">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bernegossi]]></surname>
<given-names><![CDATA[A. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Resurrection of the genus Subulo Smith, 1827 for the gray brocket deer, with designation of a neotype]]></article-title>
<source><![CDATA[Journal of Mammalogy]]></source>
<year>2022</year>
<volume>104</volume>
<page-range>619-33</page-range></nlm-citation>
</ref>
<ref id="B7">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Black-Décima]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Brown brocket deer Mazama gouazoubira (Fischer 1814). Neotropical Cervidology: Biology and Medicine of Latin American Deer. J. M. B. Duarte and S. Gonzalez]]></article-title>
<source><![CDATA[Jaboticabal, Funep/IUCN]]></source>
<year>2010</year>
<volume>190</volume>
<page-range>201</page-range></nlm-citation>
</ref>
<ref id="B8">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bodmer]]></surname>
<given-names><![CDATA[R. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Frugivory in Amazonian Artiodactyla: evidence for the evolution of the ruminant stomach]]></article-title>
<source><![CDATA[Journal of Zoology]]></source>
<year>1989</year>
<volume>219</volume>
<page-range>457-67</page-range></nlm-citation>
</ref>
<ref id="B9">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bodmer]]></surname>
<given-names><![CDATA[R. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Ungulate frugivores and the browser-grazer continuum]]></article-title>
<source><![CDATA[Oikos]]></source>
<year>1990</year>
<volume>57</volume>
<page-range>319-25</page-range></nlm-citation>
</ref>
<ref id="B10">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bodmer]]></surname>
<given-names><![CDATA[R. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Strategies of seed dispersal and seed predation in Amazonian ungulates]]></article-title>
<source><![CDATA[Biotropica]]></source>
<year>1991</year>
<volume>23</volume>
<page-range>255-61</page-range></nlm-citation>
</ref>
<ref id="B11">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bonifacino]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
<name>
<surname><![CDATA[Rossado]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<source><![CDATA[Flora &amp; Vegetación de la Reserva Natural Salus]]></source>
<year>2016</year>
<publisher-loc><![CDATA[Montevideo, Uruguay ]]></publisher-loc>
<publisher-name><![CDATA[R. N. Salus]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B12">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bridson]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
<name>
<surname><![CDATA[Forman]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[The herbarium handbook]]></source>
<year>1992</year>
<publisher-loc><![CDATA[Kew ]]></publisher-loc>
<publisher-name><![CDATA[Royal Botanics Gardens]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B13">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Caballero]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<source><![CDATA[Comparación de la dieta de la urina (Mazama gouazoupira) durante las épocas lluviosa y seca en la zona del Izozog, Santa Cruz, Bolivia]]></source>
<year>2001</year>
<publisher-loc><![CDATA[Santa Cruz, Bolivia ]]></publisher-loc>
<publisher-name><![CDATA[Universidad Autónoma Gabriel René Moreno]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B14">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cartes]]></surname>
<given-names><![CDATA[J. L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Distribución y uso de hábitat de la corzuela parda en Los Llanos de La Rioja, Argentina]]></source>
<year>1998</year>
<publisher-loc><![CDATA[Córdoba, Argentina ]]></publisher-loc>
<publisher-name><![CDATA[Universidad Nacional de Córdoba]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B15">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Cosse]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Feeding ecology of Ozotoceros bezoarticus: conservation implications in Uruguay]]></article-title>
<source><![CDATA[Iheringia. Série Zoologia]]></source>
<year>2009</year>
<volume>99</volume>
<page-range>158-64</page-range></nlm-citation>
</ref>
<ref id="B16">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Davison]]></surname>
<given-names><![CDATA[A.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[On the origin of faeces: morphological versus molecular methods for surveying rare carnivores from their scats]]></article-title>
<source><![CDATA[Journal of Zoology]]></source>
<year>2002</year>
<volume>257</volume>
<page-range>141-3</page-range></nlm-citation>
</ref>
<ref id="B17">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Deagle]]></surname>
<given-names><![CDATA[B. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data?]]></article-title>
<source><![CDATA[Molecular Ecology]]></source>
<year>2019</year>
<volume>28</volume>
<page-range>391-406</page-range></nlm-citation>
</ref>
<ref id="B18">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Deagle]]></surname>
<given-names><![CDATA[B. E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Quantifying sequence proportions in a DNA&#8208;based diet study using Ion Torrent amplicon sequencing: which counts count?]]></article-title>
<source><![CDATA[Molecular Ecology Resources]]></source>
<year>2013</year>
<volume>13</volume>
<page-range>620-33</page-range></nlm-citation>
</ref>
<ref id="B19">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Fazekas]]></surname>
<given-names><![CDATA[A. J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[DNA barcoding methods for land plants]]></article-title>
<source><![CDATA[Methods and protocols]]></source>
<year>2012</year>
<volume>858</volume>
<page-range>223-52</page-range></nlm-citation>
</ref>
<ref id="B20">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Garnick]]></surname>
<given-names><![CDATA[S.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Assessment of Animal-Based Methods Used for Estimating and Monitoring Rangeland Herbivore Diet Composition]]></article-title>
<source><![CDATA[Rangeland Ecology and Management]]></source>
<year>2018</year>
<volume>71</volume>
<page-range>449-57</page-range></nlm-citation>
</ref>
<ref id="B21">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gayot]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Comparative diet of the two forest cervids of the genus Mazama in French Guiana]]></article-title>
<source><![CDATA[Journal of Tropical Ecology]]></source>
<year>2004</year>
<volume>20</volume>
<page-range>31-43</page-range></nlm-citation>
</ref>
<ref id="B22">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Geist]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
</person-group>
<source><![CDATA[Deer of the world : their evolution, behaviour, and ecology]]></source>
<year>1998</year>
<publisher-loc><![CDATA[Mechanicsburg, U.S.A. ]]></publisher-loc>
<publisher-name><![CDATA[Stackpole Books]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B23">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hofmann]]></surname>
<given-names><![CDATA[R. R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Evolutionary steps of ecophysiological adaptation and diversification of ruminants: a comparative view of their digestive system]]></article-title>
<source><![CDATA[Oecologia]]></source>
<year>1989</year>
<volume>78</volume>
<page-range>443-57</page-range></nlm-citation>
</ref>
<ref id="B24">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Hofmann]]></surname>
<given-names><![CDATA[R. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Stewart]]></surname>
<given-names><![CDATA[D. R. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Grazer or browser: a classification based on the stomach-structure and feeding habits of East African ruminants]]></article-title>
<source><![CDATA[Mammalia]]></source>
<year>1972</year>
<volume>36</volume>
<page-range>226-40</page-range></nlm-citation>
</ref>
<ref id="B25">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Juliá]]></surname>
<given-names><![CDATA[J. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Richard]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Management of brown brocket deer (Mazama gouazoubira) under conditions of strict control in Reserva Experimental Horco Molle (Tucumán, Argentina)]]></article-title>
<source><![CDATA[Deer Specialist Group News]]></source>
<year>2001</year>
<volume>16</volume>
<page-range>8-9</page-range></nlm-citation>
</ref>
<ref id="B26">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kufner]]></surname>
<given-names><![CDATA[M. B.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Is the native deer Mazama gouazoubira threatened by competition for food with the exotic hare Lepus europaeus in the degraded Chaco in Córdoba, Argentina?]]></article-title>
<source><![CDATA[Journal of Arid Environments]]></source>
<year>2008</year>
<volume>72</volume>
<page-range>2159-67</page-range></nlm-citation>
</ref>
<ref id="B27">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Miller]]></surname>
<given-names><![CDATA[C. R.]]></given-names>
</name>
<name>
<surname><![CDATA[Waits]]></surname>
<given-names><![CDATA[L. P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The history of effective population size and genetic diversity in the Yellowstone grizzly (Ursus arctos): Implications for conservation]]></article-title>
<source><![CDATA[Proceedings of the National Academy of Sciences of the United States of America]]></source>
<year>2003</year>
<volume>100</volume>
<page-range>4334-9</page-range></nlm-citation>
</ref>
<ref id="B28">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Moreno]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Use of next-generation molecular tools in archaeological neotropical deer sample analysis]]></article-title>
<source><![CDATA[Journal of Archaeological Science: Reports]]></source>
<year>2016</year>
<volume>10</volume>
<page-range>403-10</page-range></nlm-citation>
</ref>
<ref id="B29">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Nakahara]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The applicability of DNA barcoding for dietary analysis of sika deer]]></article-title>
<source><![CDATA[DNA Barcodes]]></source>
<year>2015</year>
<volume>3</volume>
<page-range>200-6</page-range></nlm-citation>
</ref>
<ref id="B30">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Nikodemova]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The effect of low-abundance OTU filtering methods on the reliability and variability of microbial composition assessed by 16S rRNA amplicon sequencing]]></article-title>
<source><![CDATA[Front Cell Infect Microbiol]]></source>
<year>2023</year>
<volume>13</volume>
<page-range>1165295</page-range></nlm-citation>
</ref>
<ref id="B31">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pinder]]></surname>
<given-names><![CDATA[L.]]></given-names>
</name>
</person-group>
<source><![CDATA[Niche overlap among brown brocket deer, pampas deer, and cattle in the Pantanal of Brazil]]></source>
<year>1997</year>
<publisher-loc><![CDATA[U.S.A. ]]></publisher-loc>
<publisher-name><![CDATA[University of Florida]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B32">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Pompanon]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Who is eating what: diet assessment using next generation sequencing]]></article-title>
<source><![CDATA[Molecular Ecology]]></source>
<year>2012</year>
<volume>21</volume>
<page-range>1931-50</page-range></nlm-citation>
</ref>
<ref id="B33">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Prado]]></surname>
<given-names><![CDATA[H. M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Feeding ecology of five Neotropical ungulates: a critical review]]></article-title>
<source><![CDATA[Oecologia Australis]]></source>
<year>2013</year>
<volume>17</volume>
<page-range>459-73</page-range></nlm-citation>
</ref>
<ref id="B34">
<nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Putman]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<source><![CDATA[The natural history of deer]]></source>
<year>1988</year>
<publisher-loc><![CDATA[Ithaca, U.S.A. ]]></publisher-loc>
<publisher-name><![CDATA[Comstock Pub. Associates]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B35">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rayé]]></surname>
<given-names><![CDATA[G.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[New insights on diet variability revealed by DNA barcoding and high-throughput pyrosequencing: chamois diet in autumn as a case study]]></article-title>
<source><![CDATA[Ecological Research]]></source>
<year>2010</year>
<volume>26</volume>
<page-range>265-76</page-range></nlm-citation>
</ref>
<ref id="B36">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Reed]]></surname>
<given-names><![CDATA[S. P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The long&#8208;term impacts of deer herbivory in determining temperate forest stand and canopy structural complexity]]></article-title>
<source><![CDATA[Journal of Applied Ecology]]></source>
<year>2022</year>
<volume>59</volume>
<page-range>812-21</page-range></nlm-citation>
</ref>
<ref id="B37">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Richard]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
<name>
<surname><![CDATA[Fontúrbel-Rada]]></surname>
<given-names><![CDATA[F.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Análisis de las relaciones de preferencia entre frutos componentes de la dieta de la corzuela parda Mazama gouazoubira Fischer (Mammalia, Cervidae), en un ambiente secundario de Yungas (Tucumán - Argentina)]]></article-title>
<source><![CDATA[Ecología Aplicada]]></source>
<year>2006</year>
<volume>5</volume>
<page-range>1-2</page-range></nlm-citation>
</ref>
<ref id="B38">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Richard]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Hábitos frugívoros de la corzuela parda (Mazama gouazoubira, Fischer, 1814)(Mammalia: Cervidae), en un ambiente secundario de Yungas]]></article-title>
<source><![CDATA[Acta Vertebrata]]></source>
<year>1995</year>
<volume>22</volume>
<page-range>19-28</page-range></nlm-citation>
</ref>
<ref id="B39">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rognes]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[VSEARCH: a versatile open source tool for metagenomics]]></article-title>
<source><![CDATA[PeerJ]]></source>
<year>2016</year>
<volume>4</volume>
</nlm-citation>
</ref>
<ref id="B40">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Serbent]]></surname>
<given-names><![CDATA[M. P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Mazama gouazoubira (Cervidae) diet during the dry season in the arid Chaco of Córdoba (Argentina)]]></article-title>
<source><![CDATA[Journal of Arid Environments]]></source>
<year>2011</year>
<volume>75</volume>
<page-range>87-90</page-range></nlm-citation>
</ref>
<ref id="B41">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Stallings]]></surname>
<given-names><![CDATA[Jr.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Notes on feeding habits of Mazama gouazoubira in the Chaco Boreal of Paraguay]]></article-title>
<source><![CDATA[Biotropica]]></source>
<year>1984</year>
<volume>16</volume>
<page-range>155-7</page-range></nlm-citation>
</ref>
<ref id="B42">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Taberlet]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding]]></article-title>
<source><![CDATA[Nucleic Acids Research]]></source>
<year>2007</year>
<volume>35</volume>
</nlm-citation>
</ref>
<ref id="B43">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Taberlet]]></surname>
<given-names><![CDATA[P.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Noninvasive genetic sampling: look before you leap]]></article-title>
<source><![CDATA[Trends in Ecology and Evolution]]></source>
<year>1999</year>
<volume>14</volume>
<page-range>323-7</page-range></nlm-citation>
</ref>
<ref id="B44">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Tosa]]></surname>
<given-names><![CDATA[M. I.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Multi&#8208;locus DNA metabarcoding reveals seasonality of foraging ecology of western spotted skunks in the Pacific Northwest]]></article-title>
<source><![CDATA[Ecosphere]]></source>
<year>2023</year>
<volume>14</volume>
</nlm-citation>
</ref>
<ref id="B45">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Vavra]]></surname>
<given-names><![CDATA[M.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Biodiversity, exotic plant species, and herbivory: the good, the bad, and the ungulate]]></article-title>
<source><![CDATA[Forest Ecology and Management]]></source>
<year>2007</year>
<volume>246</volume>
<page-range>66-72</page-range></nlm-citation>
</ref>
<ref id="B46">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[V&#283;trovský]]></surname>
<given-names><![CDATA[T.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[SEED 2: a user-friendly platform for amplicon high-throughput sequencing data analyses]]></article-title>
<source><![CDATA[Bioinformatics]]></source>
<year>2018</year>
<volume>34</volume>
<page-range>2292-4</page-range></nlm-citation>
</ref>
<ref id="B47">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Waits]]></surname>
<given-names><![CDATA[L. P.]]></given-names>
</name>
<name>
<surname><![CDATA[Paetkau]]></surname>
<given-names><![CDATA[D.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Noninvasive genetic sampling tools for wildlife biologist: a review of applications and recommendations for accurate data collection]]></article-title>
<source><![CDATA[Journal of Wildlife Management]]></source>
<year>2005</year>
<volume>69</volume>
<page-range>1419-33</page-range></nlm-citation>
</ref>
<ref id="B48">
<nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Willerslev]]></surname>
<given-names><![CDATA[E.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Fifty thousand years of Arctic vegetation and megafaunal diet]]></article-title>
<source><![CDATA[Nature]]></source>
<year>2014</year>
<volume>506</volume>
<page-range>47-51</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
