<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-1124</journal-id>
<journal-title><![CDATA[Revista mexicana de ciencias pecuarias]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. de cienc. pecuarias]]></abbrev-journal-title>
<issn>2007-1124</issn>
<publisher>
<publisher-name><![CDATA[Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-11242021000200653</article-id>
<article-id pub-id-type="doi">10.22319/rmcp.v12i2.5635</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Optimización de un protocolo de extracción de ADN a partir de sangre bovina hemolizada y coagulada para la detección molecular de Anaplasma spp.]]></article-title>
<article-title xml:lang="en"><![CDATA[Optimization of a DNA extraction protocol for hemolyzed and coagulated bovine blood for use in molecular detection of Anaplasma spp.]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Landázuri Rafael]]></surname>
<given-names><![CDATA[Tomás Humberto]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Carrazco]]></surname>
<given-names><![CDATA[Andrés]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[León]]></surname>
<given-names><![CDATA[Renato]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vinueza]]></surname>
<given-names><![CDATA[Lenin]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Barragán]]></surname>
<given-names><![CDATA[Verónica]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad San Francisco de Quito Colegio de Ciencias Biológicas y Ambientales ]]></institution>
<addr-line><![CDATA[Quito ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Pontificia Universidad Católica del Ecuador Facultad de Ciencias Exactas y Naturales ]]></institution>
<addr-line><![CDATA[Quito ]]></addr-line>
<country>Ecuador</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad San Francisco de Quito Colegio de Ciencias de la Salud Escuela de Medicina Veterinaria]]></institution>
<addr-line><![CDATA[Quito ]]></addr-line>
<country>Ecuador</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>06</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>06</month>
<year>2021</year>
</pub-date>
<volume>12</volume>
<numero>2</numero>
<fpage>653</fpage>
<lpage>664</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-11242021000200653&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-11242021000200653&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-11242021000200653&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen La anaplasmosis es una enfermedad que puede afectar de manera significativa a la producción en el sector pecuario. Para la detección molecular por PCR de Anaplasma spp., se requiere de una extracción eficiente de ADN a partir de sangre completa, lo que a su vez depende de la calidad de la muestra de sangre. Fallas en los procedimientos de muestreo o conservación de las muestras pueden ocasionar hemolisis y coagulación de la sangre, lo que a su vez puede obstaculizar el diagnóstico. El objetivo de esta investigación fue optimizar un protocolo casero de bajo costo que utiliza resina de Chelex® 100 y permite detectar de manera eficiente a Anaplasma spp. en 30 muestras de sangre bovina hemolizada y coagulada. La eficiencia del protocolo optimizado se comparó con dos kits comerciales de extracción de ADN mostrando una alta concordancia (Kappa de Cohen = 0.72) en la detección molecular de Anaplasma spp. por PCR. Se recomienda esta metodología para superar las limitaciones de investigación que pueden surgir al extraer ADN a partir de muestras de sangre complejas.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Anaplasma spp. bacteria cause anaplasmosis, a disease which negatively affects livestock production worldwide. Molecular detection by PCR requires efficient extraction of DNA from whole blood, which in turn depends on blood sample quality. Failures in sampling procedures and/or sample storage can lead to hemolysis and blood clotting, which can hamper diagnosis. An established DNA extraction protocol using Chelex® 100 resin was modified to optimize detection of Anaplasma spp. in hemolyzed and coagulated bovine blood samples, as well as reduce its cost. The optimized protocol extracted highly pure DNA effective in PCR analysis. Efficiency of the optimized protocol was compared with two commercial DNA extraction kits. When used in PCR detection of Anaplasma spp., the concordance values for all three were high (Cohen&#8217;s Kappa = 0.72). The optimized protocol is effective at extracting DNA from complex blood samples and is much less costly than commercial methods, a clear advantage when operating under limited budgets.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Anaplasma]]></kwd>
<kwd lng="es"><![CDATA[Anaplasmosis]]></kwd>
<kwd lng="es"><![CDATA[Detección molecular]]></kwd>
<kwd lng="es"><![CDATA[Extracción de ADN]]></kwd>
<kwd lng="es"><![CDATA[Chelex® 100]]></kwd>
<kwd lng="es"><![CDATA[Sangre]]></kwd>
<kwd lng="en"><![CDATA[Anaplasma]]></kwd>
<kwd lng="en"><![CDATA[Anaplasmosis]]></kwd>
<kwd lng="en"><![CDATA[Molecular detection]]></kwd>
<kwd lng="en"><![CDATA[DNA extraction]]></kwd>
<kwd lng="en"><![CDATA[Chelex® 100]]></kwd>
<kwd lng="en"><![CDATA[Blood]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Reyna-bello]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale: Logros y retos]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[González]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Madrid]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Soto]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
</person-group>
<source><![CDATA[Logros y desafíos de la ganadería doble propósito]]></source>
<year>2014</year>
<page-range>388-95</page-range><publisher-loc><![CDATA[Maracaibo, Venezuela ]]></publisher-loc>
<publisher-name><![CDATA[Ediciones Astro Data SA]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="">
<collab>OIE</collab>
<source><![CDATA[Terrestrial manual-Bovine Anaplasmosis]]></source>
<year>2018</year>
</nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Goodger]]></surname>
<given-names><![CDATA[WJ]]></given-names>
</name>
<name>
<surname><![CDATA[Carpenter]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
<name>
<surname><![CDATA[Riemann]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Estimation of economic loss associated with anaplasmosis in California beef cattle]]></article-title>
<source><![CDATA[J Am Vet Med Assoc]]></source>
<year>1979</year>
<volume>174</volume>
<numero>12</numero>
<issue>12</issue>
<page-range>1333-6</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jonsson]]></surname>
<given-names><![CDATA[NN]]></given-names>
</name>
<name>
<surname><![CDATA[Bock]]></surname>
<given-names><![CDATA[RE]]></given-names>
</name>
<name>
<surname><![CDATA[Jorgensen]]></surname>
<given-names><![CDATA[WK]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Productivity and health effects of anaplasmosis and babesiosis on Bos indicus cattle and their crosses, and the effects of differing intensity of tick control in Australia]]></article-title>
<source><![CDATA[Vet Parasitol]]></source>
<year>2008</year>
<volume>155</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>1-9</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rodríguez]]></surname>
<given-names><![CDATA[RI]]></given-names>
</name>
<name>
<surname><![CDATA[Grisi]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez de León]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Silva]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Torres]]></surname>
<given-names><![CDATA[JF]]></given-names>
</name>
<name>
<surname><![CDATA[Fragoso]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Potential economic impact assessment for cattle parasites in Mexico]]></article-title>
<source><![CDATA[Review. Rev Mex Cienc Pecu]]></source>
<year>2017</year>
<volume>8</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>61-74</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Aubry]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
<name>
<surname><![CDATA[Geale]]></surname>
<given-names><![CDATA[DW]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A review of bovine anaplasmosis]]></article-title>
<source><![CDATA[Transbound Emerg Dis]]></source>
<year>2011</year>
<volume>58</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>1-30</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Silaghi]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Santos]]></surname>
<given-names><![CDATA[AS]]></given-names>
</name>
<name>
<surname><![CDATA[Gomes]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Christova]]></surname>
<given-names><![CDATA[I]]></given-names>
</name>
<name>
<surname><![CDATA[Matei]]></surname>
<given-names><![CDATA[IA]]></given-names>
</name>
<name>
<surname><![CDATA[Walder]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Guidelines for the direct detection of Anaplasma spp. in diagnosis and epidemiological studies]]></article-title>
<source><![CDATA[Vector-Borne Zoonotic Dis]]></source>
<year>2017</year>
<volume>17</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>12-22</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Al-Soud]]></surname>
<given-names><![CDATA[WA]]></given-names>
</name>
<name>
<surname><![CDATA[Rådström]]></surname>
<given-names><![CDATA[P]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Purification and characterization of PCR-inhibitory components in blood cells]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2001</year>
<volume>39</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>485-93</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sidstedt]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Hedman]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Romsos]]></surname>
<given-names><![CDATA[EL]]></given-names>
</name>
<name>
<surname><![CDATA[Waitara]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Wadsö]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Steffen]]></surname>
<given-names><![CDATA[CR]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Inhibition mechanisms of hemoglobin, immunoglobulin G, and whole blood in digital and real-time PCR]]></article-title>
<source><![CDATA[Anal Bioanal Chem]]></source>
<year>2018</year>
<volume>410</volume>
<numero>10</numero>
<issue>10</issue>
<page-range>2569-83</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ghaheri]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Kahkrizi]]></surname>
<given-names><![CDATA[D]]></given-names>
</name>
<name>
<surname><![CDATA[Yari]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Babaie]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Suthar]]></surname>
<given-names><![CDATA[RS]]></given-names>
</name>
<name>
<surname><![CDATA[Kazemi]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A comparative evaluation of four DNA extraction protocols from whole blood sample]]></article-title>
<source><![CDATA[Cell Mol Biol]]></source>
<year>2016</year>
<volume>62</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>119-23</page-range></nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Smith]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
<name>
<surname><![CDATA[Diggle]]></surname>
<given-names><![CDATA[MA]]></given-names>
</name>
<name>
<surname><![CDATA[Clarke]]></surname>
<given-names><![CDATA[SC]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2003</year>
<volume>41</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>2440-3</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Polski]]></surname>
<given-names><![CDATA[JM]]></given-names>
</name>
<name>
<surname><![CDATA[Kimzey]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Percival]]></surname>
<given-names><![CDATA[RW]]></given-names>
</name>
<name>
<surname><![CDATA[Grosso]]></surname>
<given-names><![CDATA[LE]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Rapid and effective processing of blood specimens for diagnostic PCR using filter paper and Chelex-100]]></article-title>
<source><![CDATA[J Clin Pathol Mol Pathol]]></source>
<year>1998</year>
<volume>51</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>215-7</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Walsh]]></surname>
<given-names><![CDATA[PS]]></given-names>
</name>
<name>
<surname><![CDATA[Metzger]]></surname>
<given-names><![CDATA[DA]]></given-names>
</name>
<name>
<surname><![CDATA[Higushi]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material]]></article-title>
<source><![CDATA[BioTechniques]]></source>
<year>2013</year>
<volume>54</volume>
<page-range>134-9</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Singh]]></surname>
<given-names><![CDATA[UA]]></given-names>
</name>
<name>
<surname><![CDATA[Kumari]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Iyengar]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin]]></article-title>
<source><![CDATA[Biol Proced Online]]></source>
<year>2018</year>
<volume>20</volume>
<page-range>12</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="">
<collab>IICA. Instituto Interamericano de Cooperación para la Agricultura</collab>
<source><![CDATA[Planes de Fincas Ganaderas en Esmeralda, Ecuador]]></source>
<year>2017</year>
</nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Zobba]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Anfossi]]></surname>
<given-names><![CDATA[AG]]></given-names>
</name>
<name>
<surname><![CDATA[Parpaglia]]></surname>
<given-names><![CDATA[MLP]]></given-names>
</name>
<name>
<surname><![CDATA[Dore]]></surname>
<given-names><![CDATA[GM]]></given-names>
</name>
<name>
<surname><![CDATA[Chessa]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Spezzigu]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Molecular investigation and phylogeny of Anaplasma spp. in mediterranean ruminants reveal the presence of neutrophil-tropic strains closely related to A. platys]]></article-title>
<source><![CDATA[Appl Environ Microbiol]]></source>
<year>2014</year>
<volume>80</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>271-80</page-range></nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Du Breuil]]></surname>
<given-names><![CDATA[RM]]></given-names>
</name>
<name>
<surname><![CDATA[Patel]]></surname>
<given-names><![CDATA[JM]]></given-names>
</name>
<name>
<surname><![CDATA[Mendelow]]></surname>
<given-names><![CDATA[BV]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Quantitation of &#946;-actin-specific mRNA transcripts using xeno-competitive PCR]]></article-title>
<source><![CDATA[Genome Res]]></source>
<year>1993</year>
<volume>3</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>57-9</page-range></nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="book">
<collab>R Core Team</collab>
<source><![CDATA[R: A language and environment for statistical computing]]></source>
<year>2020</year>
<publisher-loc><![CDATA[Vienna, Austria ]]></publisher-loc>
<publisher-name><![CDATA[R Foundation for Statistical Computing]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B19">
<label>19</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[McLaughlin]]></surname>
<given-names><![CDATA[MJ]]></given-names>
</name>
<name>
<surname><![CDATA[Sainani]]></surname>
<given-names><![CDATA[KL]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Bonferroni, holm, and hochberg corrections: Fun names, serious changes to P values]]></article-title>
<source><![CDATA[PM R]]></source>
<year>2014</year>
<volume>6</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>544-6</page-range></nlm-citation>
</ref>
<ref id="B20">
<label>20</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[McHugh]]></surname>
<given-names><![CDATA[ML]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Interrater reliability: The kappa statistic]]></article-title>
<source><![CDATA[Biochem Medica]]></source>
<year>2012</year>
<volume>22</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>276-82</page-range></nlm-citation>
</ref>
<ref id="B21">
<label>21</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sánchez-Otero]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
</person-group>
<source><![CDATA[Introducción a la estadística no paramétrica y al análisis multivariado]]></source>
<year>2016</year>
<page-range>12-3</page-range><publisher-loc><![CDATA[Quito, Ecuador ]]></publisher-loc>
<publisher-name><![CDATA[Giro Creativo]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B22">
<label>22</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Suarez]]></surname>
<given-names><![CDATA[CE]]></given-names>
</name>
<name>
<surname><![CDATA[Noh]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Emerging perspectives in the research of bovine babesiosis and anaplasmosis]]></article-title>
<source><![CDATA[Vet Parasitol]]></source>
<year>2011</year>
<volume>180</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>109-25</page-range></nlm-citation>
</ref>
<ref id="B23">
<label>23</label><nlm-citation citation-type="">
<collab>FAO</collab>
<source><![CDATA[Ganadería de América Latina y el Caribe puede jugar rol clave en el logro de los Objetivos de Desarrollo Sostenible]]></source>
<year>2016</year>
</nlm-citation>
</ref>
<ref id="B24">
<label>24</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Donadeu]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Nwankpa]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Abela-Ridder]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Dungu]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Strategies to increase adoption of animal vaccines by smallholder farmers with focus on neglected diseases and marginalized populations]]></article-title>
<source><![CDATA[PLoS Negl Trop Dis]]></source>
<year>2019</year>
<volume>13</volume>
<numero>2</numero>
<issue>2</issue>
</nlm-citation>
</ref>
<ref id="B25">
<label>25</label><nlm-citation citation-type="">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rodríguez]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
</person-group>
<source><![CDATA[La investigación científica en Ecuador es cinco veces más cara por costos de reactivos y equipos]]></source>
<year>2019</year>
</nlm-citation>
</ref>
<ref id="B26">
<label>26</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Dong]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Ismail]]></surname>
<given-names><![CDATA[N]]></given-names>
</name>
<name>
<surname><![CDATA[Walker]]></surname>
<given-names><![CDATA[DH]]></given-names>
</name>
<name>
<surname><![CDATA[Coleman]]></surname>
<given-names><![CDATA[W]]></given-names>
</name>
<name>
<surname><![CDATA[Tsongalis]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<source><![CDATA[Molecular testing in emerging infectious diseases]]></source>
<year>2016</year>
<publisher-loc><![CDATA[Londres, Reino Unido ]]></publisher-loc>
<publisher-name><![CDATA[Elsevier Academic Press]]></publisher-name>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
