<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-1124</journal-id>
<journal-title><![CDATA[Revista mexicana de ciencias pecuarias]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. mex. de cienc. pecuarias]]></abbrev-journal-title>
<issn>2007-1124</issn>
<publisher>
<publisher-name><![CDATA[Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-11242018000400769</article-id>
<article-id pub-id-type="doi">10.22319/rmcp.v9i4.4508</article-id>
<title-group>
<article-title xml:lang="en"><![CDATA[Immunolocalization of VirB11 protein in the Anaplasma marginale outer membrane and its reaction with bovine immune sera]]></article-title>
<article-title xml:lang="es"><![CDATA[Inmunolocalización de la proteína VirB11 en la membrana externa de Anaplasma marginale y su reacción con sueros inmunes de bovino]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Barrera Molina]]></surname>
<given-names><![CDATA[América Ivette]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
<xref ref-type="aff" rid="Aaf"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Cossío Bayúgar]]></surname>
<given-names><![CDATA[Raquel]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Gutiérrez-Pabello]]></surname>
<given-names><![CDATA[José A.]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Tello López]]></surname>
<given-names><![CDATA[Ángel Tolentino]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Preciado de la Torre]]></surname>
<given-names><![CDATA[Jesús Francisco]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Rodríguez Camarillo]]></surname>
<given-names><![CDATA[Sergio Darío]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Nacional Autónoma de México  ]]></institution>
<addr-line><![CDATA[ Ciudad de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,INIFAP Centro de Nacional de Investigación Disciplinaria en Parasitología Veterinaria ]]></institution>
<addr-line><![CDATA[ Mor]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Instituto Nacional de Salud Pública  ]]></institution>
<addr-line><![CDATA[ Mor]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2018</year>
</pub-date>
<volume>9</volume>
<numero>4</numero>
<fpage>769</fpage>
<lpage>791</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-11242018000400769&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-11242018000400769&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-11242018000400769&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Type IV secretion system is composed by a group of proteins associated with substrate transfer between bacteria and eukaryotic organisms. VirB11 is a protein of this system and, in A. marginale it induces partial protection in cattle immunized with external membrane fragments of the rickettsia. The objectives of this study were: to determine the conservation rate of VirB11 among different Mexican isolates and to corroborate its cellular localization on the outer membrane of A. marginale. Nucleotide and amino acid sequence alignment of virB11 from nine Mexican isolates showed 100 % identity. Bovine sera from both naturally and experimentally infected cattle reacted against a multiple antigenic peptide (MAP-VirB11) designed based on a B-type epitope present in VirB11. In addition, MAP-VirB11-rabbit immune sera reacted with native VirB11 by ELISA and western blot in rickettsial extracts and on the surface of bovine infected erythrocytes by indirect immunofluorescence and the localization of VirB11 protein was determined on the outer surface of A. marginale initial bodies.]]></p></abstract>
<abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: El sistema de secreción tipo IV es un grupo de proteínas asociadas a la transferencia de sustratos entre bacterias y organismos eucariontes. VirB11 es una proteína de este sistema y en A. marginale induce protección parcial en bovinos inmunizados con fragmentos de membrana externa de la rickettsia. Los objetivos del presente trabajo fueron, determinar el índice de conservación de la proteína VirB11 entre diferentes aislados de México y, corroborar su localización celular en la membrana externa de A. marginale. El alineamiento de las secuencias de nucleótidos de virB11 de nueve aislados mexicanos mostró un 100 % de identidad. Sueros de bovinos infectados de manera natural y experimental, reaccionaron contra un péptido antigénico múltiple (MAP-VirB11) diseñado a partir de un epítopo tipo B presente en VirB11. Adicionalmente, sueros de conejos inmunizados con MAP-VirB11 reaccionaron con VirB11 nativa por ELISA y western blot en extractos de la rickettsia y en la superficie de eritrocitos infectados de bovinos por inmunofluorescencia indirecta y, se logró determinar la localización de la proteína VirB11 sobre la superficie exterior de cuerpos iniciales de A. marginale.]]></p></abstract>
<kwd-group>
<kwd lng="en"><![CDATA[Anaplasma marginale]]></kwd>
<kwd lng="en"><![CDATA[Type 4 secretion system]]></kwd>
<kwd lng="en"><![CDATA[Multiple antigenic peptide]]></kwd>
<kwd lng="en"><![CDATA[MAP]]></kwd>
<kwd lng="en"><![CDATA[VirB11]]></kwd>
<kwd lng="es"><![CDATA[Anaplasma marginale]]></kwd>
<kwd lng="es"><![CDATA[Sistema de Secreción 4]]></kwd>
<kwd lng="es"><![CDATA[Péptido antigénico múltiple (MAP)]]></kwd>
<kwd lng="es"><![CDATA[MAPVirB11]]></kwd>
</kwd-group>
</article-meta>
</front><back>
<ref-list>
<ref id="B1">
<label>1</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kocan]]></surname>
<given-names><![CDATA[KML]]></given-names>
</name>
<name>
<surname><![CDATA[de la Fuente]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Guglielmone]]></surname>
<given-names><![CDATA[AA]]></given-names>
</name>
<name>
<surname><![CDATA[Meléndez]]></surname>
<given-names><![CDATA[RD.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Antigens and alternatives for control of Anaplasma marginale infection in cattle]]></article-title>
<source><![CDATA[Clin Microbiol Rev]]></source>
<year>2003</year>
<volume>16</volume>
<numero>4</numero>
<issue>4</issue>
<page-range>698-712</page-range></nlm-citation>
</ref>
<ref id="B2">
<label>2</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rodríguez-Vivas]]></surname>
<given-names><![CDATA[RI]]></given-names>
</name>
<name>
<surname><![CDATA[Grisi]]></surname>
<given-names><![CDATA[L]]></given-names>
</name>
<name>
<surname><![CDATA[Pérez de León]]></surname>
<given-names><![CDATA[AA]]></given-names>
</name>
<name>
<surname><![CDATA[Silva Villela]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[de Jesús Torres-Acosta]]></surname>
<given-names><![CDATA[JF]]></given-names>
</name>
<name>
<surname><![CDATA[Fragoso Sánchez]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Potential economic impact assessment for cattle parasites in Mexico]]></article-title>
<source><![CDATA[Rev Mex Cienc Pecu]]></source>
<year>2017</year>
<volume>8</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>61-74</page-range></nlm-citation>
</ref>
<ref id="B3">
<label>3</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Rodríguez]]></surname>
<given-names><![CDATA[CSD]]></given-names>
</name>
<name>
<surname><![CDATA[García]]></surname>
<given-names><![CDATA[OMA]]></given-names>
</name>
<name>
<surname><![CDATA[Rojas]]></surname>
<given-names><![CDATA[REE]]></given-names>
</name>
<name>
<surname><![CDATA[Cantó]]></surname>
<given-names><![CDATA[AGJ]]></given-names>
</name>
<name>
<surname><![CDATA[Preciado]]></surname>
<given-names><![CDATA[TJF]]></given-names>
</name>
<name>
<surname><![CDATA[Rosario]]></surname>
<given-names><![CDATA[CR]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale Yucatan (Mexico) Strain. Assessment of low virulence and potential use as a live vaccine]]></article-title>
<source><![CDATA[Ann N Y Acad Sci]]></source>
<year>2008</year>
<volume>1149</volume>
<page-range>98-102</page-range></nlm-citation>
</ref>
<ref id="B4">
<label>4</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Kocan KM]]></surname>
<given-names><![CDATA[de la Fuente J]]></given-names>
</name>
<name>
<surname><![CDATA[Blouin]]></surname>
<given-names><![CDATA[EF]]></given-names>
</name>
<name>
<surname><![CDATA[Coetzee JF]]></surname>
<given-names><![CDATA[Ewing SA]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The natural history of Anaplasma marginale]]></article-title>
<source><![CDATA[Vet Parasitol]]></source>
<year>2010</year>
<volume>167</volume>
<page-range>95-107</page-range></nlm-citation>
</ref>
<ref id="B5">
<label>5</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Ueti]]></surname>
<given-names><![CDATA[MW]]></given-names>
</name>
<name>
<surname><![CDATA[Reagan]]></surname>
<given-names><![CDATA[JO]]></given-names>
</name>
<name>
<surname><![CDATA[Knowles]]></surname>
<given-names><![CDATA[DP]]></given-names>
</name>
<name>
<surname><![CDATA[Scoles]]></surname>
<given-names><![CDATA[GA]]></given-names>
</name>
<name>
<surname><![CDATA[Shkap]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Palmer]]></surname>
<given-names><![CDATA[GH.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale]]></article-title>
<source><![CDATA[Infect Immun]]></source>
<year>2007</year>
<volume>75</volume>
<page-range>2959-64</page-range></nlm-citation>
</ref>
<ref id="B6">
<label>6</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Palmer]]></surname>
<given-names><![CDATA[GH]]></given-names>
</name>
<name>
<surname><![CDATA[Knowles]]></surname>
<given-names><![CDATA[DP]]></given-names>
</name>
<name>
<surname><![CDATA[Rodriguez]]></surname>
<given-names><![CDATA[JL]]></given-names>
</name>
<name>
<surname><![CDATA[Gnad]]></surname>
<given-names><![CDATA[DP]]></given-names>
</name>
<name>
<surname><![CDATA[Hollis]]></surname>
<given-names><![CDATA[LC]]></given-names>
</name>
<name>
<surname><![CDATA[Marston]]></surname>
<given-names><![CDATA[T]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Stochastic transmission of multiple genotypically distinct Anaplasma marginale strains in a herd with high prevalence of Anaplasma infection]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2004</year>
<volume>42</volume>
<numero>11</numero>
<issue>11</issue>
<page-range>5381-4</page-range></nlm-citation>
</ref>
<ref id="B7">
<label>7</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Palmer]]></surname>
<given-names><![CDATA[GH]]></given-names>
</name>
<name>
<surname><![CDATA[McGuire]]></surname>
<given-names><![CDATA[TC.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Immune serum against Anaplasma marginale initial bodies neutralizes infectivity for cattle]]></article-title>
<source><![CDATA[J Immunol]]></source>
<year>1984</year>
<volume>133</volume>
<page-range>1010-5</page-range></nlm-citation>
</ref>
<ref id="B8">
<label>8</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[De la Fuente]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Van Den Bussche]]></surname>
<given-names><![CDATA[RA]]></given-names>
</name>
<name>
<surname><![CDATA[Prado]]></surname>
<given-names><![CDATA[TM]]></given-names>
</name>
<name>
<surname><![CDATA[Kocan]]></surname>
<given-names><![CDATA[KM.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale msp1&#945; genotypes evolved under positive selection pressure but are not markers for geographic isolates]]></article-title>
<source><![CDATA[J Clin Microbiol]]></source>
<year>2003</year>
<volume>41</volume>
<page-range>1609-16</page-range></nlm-citation>
</ref>
<ref id="B9">
<label>9</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Brayton]]></surname>
<given-names><![CDATA[KA]]></given-names>
</name>
<name>
<surname><![CDATA[Kappmeyer]]></surname>
<given-names><![CDATA[LS]]></given-names>
</name>
<name>
<surname><![CDATA[Herndon]]></surname>
<given-names><![CDATA[DR]]></given-names>
</name>
<name>
<surname><![CDATA[Dark]]></surname>
<given-names><![CDATA[MJ]]></given-names>
</name>
<name>
<surname><![CDATA[Tibbals]]></surname>
<given-names><![CDATA[DL]]></given-names>
</name>
<name>
<surname><![CDATA[Palmer]]></surname>
<given-names><![CDATA[GH]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Complete genome sequencing of Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins]]></article-title>
<source><![CDATA[Proc Natl Acad Sci USA]]></source>
<year>2005</year>
<volume>102</volume>
<page-range>844-9</page-range></nlm-citation>
</ref>
<ref id="B10">
<label>10</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Castañeda-Ortiz]]></surname>
<given-names><![CDATA[EJ]]></given-names>
</name>
<name>
<surname><![CDATA[Ueti]]></surname>
<given-names><![CDATA[MW]]></given-names>
</name>
<name>
<surname><![CDATA[Camacho-Nuez]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Mosqueda]]></surname>
<given-names><![CDATA[JJ]]></given-names>
</name>
<name>
<surname><![CDATA[Mousel]]></surname>
<given-names><![CDATA[MR]]></given-names>
</name>
<name>
<surname><![CDATA[Johnson]]></surname>
<given-names><![CDATA[WC]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Association of Anaplasma marginale strain superinfection with infection prevalence within tropical regions]]></article-title>
<source><![CDATA[PLoS One]]></source>
<year>2015</year>
<volume>10</volume>
<numero>5</numero>
<issue>5</issue>
</nlm-citation>
</ref>
<ref id="B11">
<label>11</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sutten]]></surname>
<given-names><![CDATA[EL]]></given-names>
</name>
<name>
<surname><![CDATA[Norimine]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Beare]]></surname>
<given-names><![CDATA[PA]]></given-names>
</name>
<name>
<surname><![CDATA[Heinzen]]></surname>
<given-names><![CDATA[RA]]></given-names>
</name>
<name>
<surname><![CDATA[Lopez]]></surname>
<given-names><![CDATA[JE]]></given-names>
</name>
<name>
<surname><![CDATA[Morse]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine]]></article-title>
<source><![CDATA[Infect Immun]]></source>
<year>2010</year>
<volume>78</volume>
<page-range>1314-25</page-range></nlm-citation>
</ref>
<ref id="B12">
<label>12</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[González-Pedrajo]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Dreyfus]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Sistemas de secreción de proteínas en las bacterias Gram negativas: Biogénesis flagelar y translocación de factores de virulencia]]></article-title>
<source><![CDATA[Mensaje Bioquim]]></source>
<year>2003</year>
<volume>27</volume>
<page-range>45-63</page-range></nlm-citation>
</ref>
<ref id="B13">
<label>13</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sutten]]></surname>
<given-names><![CDATA[EL]]></given-names>
</name>
<name>
<surname><![CDATA[Norimine]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Beare]]></surname>
<given-names><![CDATA[PA]]></given-names>
</name>
<name>
<surname><![CDATA[Heinzen]]></surname>
<given-names><![CDATA[RA]]></given-names>
</name>
<name>
<surname><![CDATA[Lopez]]></surname>
<given-names><![CDATA[JE]]></given-names>
</name>
<name>
<surname><![CDATA[Morse]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine]]></article-title>
<source><![CDATA[Infect Immun]]></source>
<year>2010</year>
<volume>78</volume>
<page-range>1314-25</page-range></nlm-citation>
</ref>
<ref id="B14">
<label>14</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Apostolopoulos]]></surname>
<given-names><![CDATA[V.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Vaccine delivery methods into the future]]></article-title>
<source><![CDATA[Vaccines (Basel)]]></source>
<year>2016</year>
<volume>4</volume>
<numero>2</numero>
<issue>2</issue>
<page-range>9</page-range></nlm-citation>
</ref>
<ref id="B15">
<label>15</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Jiménez Ocampo]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Rodríguez Camarillo]]></surname>
<given-names><![CDATA[SD]]></given-names>
</name>
<name>
<surname><![CDATA[Rosario]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Orozco Vega]]></surname>
<given-names><![CDATA[LE]]></given-names>
</name>
<name>
<surname><![CDATA[de la Fuente]]></surname>
<given-names><![CDATA[J.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale: análisis de las secuencias del fragmento variable del gen msp1 &#945; y del gen msp4 de cuatro nuevas cepas mexicanas]]></article-title>
<source><![CDATA[Téc Pecu Méx]]></source>
<year>2008</year>
<volume>46</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>69-78</page-range></nlm-citation>
</ref>
<ref id="B16">
<label>16</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Bradford]]></surname>
<given-names><![CDATA[MM.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding]]></article-title>
<source><![CDATA[Ann Biochem]]></source>
<year>1976</year>
<volume>72</volume>
<page-range>248-54</page-range></nlm-citation>
</ref>
<ref id="B17">
<label>17</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Eddy]]></surname>
<given-names><![CDATA[SR.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Multiple alignment using hidden Markov models]]></article-title>
<source><![CDATA[Proc Int Conf Intell Syst Mol Biol]]></source>
<year>1995</year>
<volume>3</volume>
<page-range>114-20</page-range></nlm-citation>
</ref>
<ref id="B18">
<label>18</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Möller]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Croning]]></surname>
<given-names><![CDATA[MD]]></given-names>
</name>
<name>
<surname><![CDATA[Apweiler]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Evaluation of methods for the prediction of membrane spanning regions]]></article-title>
<source><![CDATA[Bioinformatics]]></source>
<year>2001</year>
<volume>17</volume>
<numero>7</numero>
<issue>7</issue>
<page-range>646-53</page-range></nlm-citation>
</ref>
<ref id="B19">
<label>19</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gasteiger]]></surname>
<given-names><![CDATA[E]]></given-names>
</name>
<name>
<surname><![CDATA[Hoogland]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Gattiker]]></surname>
<given-names><![CDATA[A]]></given-names>
</name>
<name>
<surname><![CDATA[Duvaud]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Wilkins]]></surname>
<given-names><![CDATA[MR]]></given-names>
</name>
<name>
<surname><![CDATA[Appel]]></surname>
<given-names><![CDATA[RD]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Protein Identification and analysis tools on the ExPASy Server]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Walker]]></surname>
<given-names><![CDATA[John M.]]></given-names>
</name>
</person-group>
<source><![CDATA[The proteomics protocols handbook]]></source>
<year>2005</year>
<page-range>571-607</page-range><publisher-loc><![CDATA[USA ]]></publisher-loc>
<publisher-name><![CDATA[Humana Press]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B20">
<label>20</label><nlm-citation citation-type="book">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Saha]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Raghava]]></surname>
<given-names><![CDATA[GPS.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[BcePred: Prediction of continuous B-cell epitopes in antigenic sequences using physico-chemical properties]]></article-title>
<person-group person-group-type="editor">
<name>
<surname><![CDATA[Nicosia]]></surname>
<given-names><![CDATA[G]]></given-names>
</name>
</person-group>
<source><![CDATA[Artificial Immune Systems]]></source>
<year>2004</year>
<page-range>197-204</page-range><publisher-loc><![CDATA[Germany ]]></publisher-loc>
<publisher-name><![CDATA[Springer]]></publisher-name>
</nlm-citation>
</ref>
<ref id="B21">
<label>21</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Saha]]></surname>
<given-names><![CDATA[S]]></given-names>
</name>
<name>
<surname><![CDATA[Raghava]]></surname>
<given-names><![CDATA[GP.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Prediction of continuous B-cell epitopes in an antigen using recurrent neural network]]></article-title>
<source><![CDATA[Proteins]]></source>
<year>2006</year>
<volume>65</volume>
<numero>1</numero>
<issue>1</issue>
<page-range>40-8</page-range></nlm-citation>
</ref>
<ref id="B22">
<label>22</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Oliveira]]></surname>
<given-names><![CDATA[B]]></given-names>
</name>
<name>
<surname><![CDATA[Araújo]]></surname>
<given-names><![CDATA[R]]></given-names>
</name>
<name>
<surname><![CDATA[Melo]]></surname>
<given-names><![CDATA[V]]></given-names>
</name>
<name>
<surname><![CDATA[Madruga]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Soares]]></surname>
<given-names><![CDATA[C]]></given-names>
</name>
<name>
<surname><![CDATA[Kessler]]></surname>
<given-names><![CDATA[R.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Development of enzyme-linked immunosorbent assays based on recombinant MSP1a and MSP2 of Anaplasma marginale]]></article-title>
<source><![CDATA[Mem Inst Oswaldo Cruz]]></source>
<year>2005</year>
<volume>100</volume>
<numero>7</numero>
<issue>7</issue>
<page-range>765-9</page-range></nlm-citation>
</ref>
<ref id="B23">
<label>23</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Brayton]]></surname>
<given-names><![CDATA[KA]]></given-names>
</name>
<name>
<surname><![CDATA[Kappmeyer]]></surname>
<given-names><![CDATA[LS]]></given-names>
</name>
<name>
<surname><![CDATA[Herdon]]></surname>
<given-names><![CDATA[DR]]></given-names>
</name>
<name>
<surname><![CDATA[Dark]]></surname>
<given-names><![CDATA[MJ]]></given-names>
</name>
<name>
<surname><![CDATA[Tibbals]]></surname>
<given-names><![CDATA[DL]]></given-names>
</name>
<name>
<surname><![CDATA[Palmer]]></surname>
<given-names><![CDATA[GH]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Complete genome sequencing or Anaplasma marginale reveals that the surface is skewed to two superfamilies of outer membrane proteins]]></article-title>
<source><![CDATA[Proc Natl Acad Sci]]></source>
<year>2005</year>
<volume>102</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>844-9</page-range></nlm-citation>
</ref>
<ref id="B24">
<label>24</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Sutten]]></surname>
<given-names><![CDATA[EL]]></given-names>
</name>
<name>
<surname><![CDATA[Norimine]]></surname>
<given-names><![CDATA[J]]></given-names>
</name>
<name>
<surname><![CDATA[Beare]]></surname>
<given-names><![CDATA[PA]]></given-names>
</name>
<name>
<surname><![CDATA[Heinzen]]></surname>
<given-names><![CDATA[RA]]></given-names>
</name>
<name>
<surname><![CDATA[Lopez]]></surname>
<given-names><![CDATA[JE]]></given-names>
</name>
<name>
<surname><![CDATA[Morse]]></surname>
<given-names><![CDATA[K]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Anaplasma marginale type IV secretion system proteins VirB2, VirB7, VirB11, and VirD4 are immunogenic components of a protective bacterial membrane vaccine]]></article-title>
<source><![CDATA[Infect Immun]]></source>
<year>2010</year>
<volume>78</volume>
<page-range>1314-25</page-range></nlm-citation>
</ref>
<ref id="B25">
<label>25</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Gillespie]]></surname>
<given-names><![CDATA[JJ]]></given-names>
</name>
<name>
<surname><![CDATA[Phan]]></surname>
<given-names><![CDATA[IQH]]></given-names>
</name>
<name>
<surname><![CDATA[Driscoll]]></surname>
<given-names><![CDATA[TP]]></given-names>
</name>
<name>
<surname><![CDATA[Guillotte]]></surname>
<given-names><![CDATA[ML]]></given-names>
</name>
<name>
<surname><![CDATA[Lehman]]></surname>
<given-names><![CDATA[SS]]></given-names>
</name>
<name>
<surname><![CDATA[Rennoll-Bankert]]></surname>
<given-names><![CDATA[KE]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[The Rickettsia type IV secretion system: Unrealized complexity mired by gene family expansion]]></article-title>
<source><![CDATA[Pathog Dis]]></source>
<year>2016</year>
<volume>74</volume>
<numero>6</numero>
<issue>6</issue>
<page-range>ftw058</page-range></nlm-citation>
</ref>
<ref id="B26">
<label>26</label><nlm-citation citation-type="journal">
<person-group person-group-type="author">
<name>
<surname><![CDATA[Niu]]></surname>
<given-names><![CDATA[H]]></given-names>
</name>
<name>
<surname><![CDATA[Rikihisa]]></surname>
<given-names><![CDATA[Y]]></given-names>
</name>
<name>
<surname><![CDATA[Yamaguchi]]></surname>
<given-names><![CDATA[M]]></given-names>
</name>
<name>
<surname><![CDATA[Ohashi]]></surname>
<given-names><![CDATA[N.]]></given-names>
</name>
</person-group>
<article-title xml:lang=""><![CDATA[Differential expression of VirB9 and VirB6 during the life cycle of Anaplasma phagocytophilum in human leucocytes is associated with differential binding and avoidance of lysosome pathway]]></article-title>
<source><![CDATA[Cell Microbiol]]></source>
<year>2006</year>
<volume>8</volume>
<numero>3</numero>
<issue>3</issue>
<page-range>523-34</page-range></nlm-citation>
</ref>
</ref-list>
</back>
</article>
