<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-0934</journal-id>
<journal-title><![CDATA[Revista mexicana de ciencias agrícolas]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Mex. Cienc. Agríc]]></abbrev-journal-title>
<issn>2007-0934</issn>
<publisher>
<publisher-name><![CDATA[Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-09342019000601449</article-id>
<article-id pub-id-type="doi">10.29312/remexca.v10i6.1519</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Tomato spotted wilt orthotospovirus (TSWV) no se trasmite por semilla de jitomate]]></article-title>
<article-title xml:lang="en"><![CDATA[Tomato spotted wilt orthotospovirus (TSWV) is not transmitted by tomato seed]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ríos Domínguez]]></surname>
<given-names><![CDATA[Guadalupe]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Salgado Siclán]]></surname>
<given-names><![CDATA[Martha Lidya]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aguilar Ortigoza]]></surname>
<given-names><![CDATA[Carlos]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Aquino Martínez]]></surname>
<given-names><![CDATA[Jesús Gaudencio]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Ramírez Dávila]]></surname>
<given-names><![CDATA[José Francisco]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Universidad Autónoma del Estado de México Ciencias Agropecuarias y Recursos Naturales Facultad de Ciencias Agrícolas]]></institution>
<addr-line><![CDATA[ Toluca]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Universidad Autónoma del Estado de México Facultad de Ciencias Agrícolas Campus &#8216;El Cerrillo&#8217;]]></institution>
<addr-line><![CDATA[ Toluca]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Universidad Autónoma del Estado de México Facultad de Ciencias Campus &#8216;El Cerrillo&#8217;]]></institution>
<addr-line><![CDATA[ Toluca]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Instituto de Investigación y Capacitación Agropecuaria, Acuícola y Forestal del Estado de México  ]]></institution>
<addr-line><![CDATA[Metepec ]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>00</month>
<year>2019</year>
</pub-date>
<volume>10</volume>
<numero>6</numero>
<fpage>1449</fpage>
<lpage>1455</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-09342019000601449&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-09342019000601449&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-09342019000601449&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen A la fecha, se reportan más de 231 virus capaces de ser transmitidos por semilla de manera externa o interna, esta transmisión constituye uno de los factores más importantes en el desarrollo epidemiológico de enfermedades de origen viral de precoz infección. El virus marchitez manchada del tomate tomato spot wilt orthotospovirus (TSWV), Orthotospoviridae) afecta a más de 900 familias de plantas incluyendo solanáceas, su principal transmisión es por insectos vectores como los trips, sin embargo, poco se conoce de su posible transmisión a través de semilla. El objetivo del presente trabajo fue determinar la posible trasmisión del TSWV por semilla de plantas de jitomate infectadas empleando técnicas serológicas y RT-PCR en su detección. Se colectaron frutos de jitomate provenientes de plantas infectadas de manera natural con TSWV previamente analizadas por Das-Elisa y positivas al virus de cuatro regiones del Estado de México. Posteriormente, se extrajeron las semillas de los frutos enfermos, a un grupo se les realizó la detección del virus por Das-Elisa en embrión y testa y el otro grupo de semillas se desarrollaron en cultivo in vitro por 15 días. Una vez obtenidas las plántulas, se llevó a cabo la extracción de ARN y la RT-PCR, usando los oligos específicos a la nucleocápside. Los análisis de embrión, semilla y plántulas in vitro con Das- Elisa y RT-PCR (respectivamente) resultaron ser negativos a TSWV en las muestras tratadas. Al parecer no existe infección por el virus en embrión, testa y plántulas in vitro de jitomate.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract To date, more than 231 viruses capable of being transmitted by seed are reported externally or internally, this transmission is one of the most important factors in the epidemiological development of diseases of viral origin of early infection. The spotted wilt virus of the tomato spot wilt orthotospovirus (TSWV), Orthotospoviridae) affects more than 900 plant families including Solanaceae, its main transmission is by vector insects such as thrips, however, little is known of its possible transmission through of seed. The objective of this work was to determine the possible transmission of TSWV by seed of infected tomato plants using serological techniques and RT-PCR in its detection. Tomato fruits were collected from plants naturally infected with TSWV previously analyzed by Das-Elisa and positive for the virus from four regions of the State of Mexico. Subsequently, the seeds of the diseased fruits were extracted, one group had the detection of the virus by Das-Elisa in embryo and testa and the other group of seeds were grown in vitro culture for 15 days. Once the seedlings were obtained, RNA extraction and RT-PCR were carried out, using nucleocapsid-specific oligos. In vitro embryo, seed and seedling analyzes with Das-Elisa and RT-PCR (respectively) were found to be negative for TSWV in the treated samples. Apparently, there is no virus infection in in vitro embryo, testa and tomato seedlings.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Solanum lycopersicum L.]]></kwd>
<kwd lng="es"><![CDATA[in vitro]]></kwd>
<kwd lng="es"><![CDATA[Das-Elisa]]></kwd>
<kwd lng="es"><![CDATA[RT-PCR]]></kwd>
<kwd lng="es"><![CDATA[semilla]]></kwd>
<kwd lng="es"><![CDATA[TSWV]]></kwd>
<kwd lng="en"><![CDATA[Solanum lycopersicum L.]]></kwd>
<kwd lng="en"><![CDATA[in vitro]]></kwd>
<kwd lng="en"><![CDATA[Das-Elisa]]></kwd>
<kwd lng="en"><![CDATA[RT-PCR]]></kwd>
<kwd lng="en"><![CDATA[seed]]></kwd>
<kwd lng="en"><![CDATA[TSWV]]></kwd>
</kwd-group>
</article-meta>
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<publisher-name><![CDATA[APS PRESS]]></publisher-name>
</nlm-citation>
</ref>
</ref-list>
</back>
</article>
