<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>2007-0934</journal-id>
<journal-title><![CDATA[Revista mexicana de ciencias agrícolas]]></journal-title>
<abbrev-journal-title><![CDATA[Rev. Mex. Cienc. Agríc]]></abbrev-journal-title>
<issn>2007-0934</issn>
<publisher>
<publisher-name><![CDATA[Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S2007-09342016000601321</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Capacidad predictiva de DAS-ELISA al virus mosaico estriado de la cebada en trigo]]></article-title>
<article-title xml:lang="en"><![CDATA[Predictive capability of DAS-ELISA to barley stripe mosaic virus in wheat]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Martínez-Mirafuentes]]></surname>
<given-names><![CDATA[Arturo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Valencia-Torres]]></surname>
<given-names><![CDATA[Noemí]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mezzalama]]></surname>
<given-names><![CDATA[Mónica]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Vargas-Hernández]]></surname>
<given-names><![CDATA[Mateo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Hernández-Anguiano]]></surname>
<given-names><![CDATA[Ana María]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,Colegio de Postgraduados  ]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Centro Internacional de Mejoramiento de Maíz y Trigo  ]]></institution>
<addr-line><![CDATA[ Estado de México]]></addr-line>
<country>Mexico</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>09</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>09</month>
<year>2016</year>
</pub-date>
<volume>7</volume>
<numero>6</numero>
<fpage>1321</fpage>
<lpage>1333</lpage>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_arttext&amp;pid=S2007-09342016000601321&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_abstract&amp;pid=S2007-09342016000601321&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.org.mx/scielo.php?script=sci_pdf&amp;pid=S2007-09342016000601321&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen La detección de patógenos asociados a semilla requiere contar con muestras representativas y homogéneas para obtener resultados confiables. Esta investigación tuvo como objetivos evaluar si: 1) el nivel de infección ( 5, 10 y 15 %); 2) el incremento del número de muestra simples (10, 15, 20 y 25), utilizadas para formar muestras compuestas; y 3) el incremento y la reducción del tamaño de muestra usual de 10% (establecido en el CIMMYT para el diagnóstico de plagas y enfermedades) a 15 y 5%, afectan la capacidad predictiva de la prueba DAS-ELISA al virus del mosaico estriado de la cebada (BSMV, por sus siglas en ingles). A partir de lotes de semilla de trigo harinero (Triticum aestivum L.), uno naturalmente infectado y otro sano, se formaron 210 muestras simples (MS), de 20 g cada una, divididas en grupos iguales de 70 muestras MS con 5, 10 y 15% de nivel de infección (NI) para formar tamaños de muestra (TM) de 15, 10 y 5%, y muestras compuestas (MC) con 10, 15, 20 y 25 muestras MS por NI y TM. Los resultados indicaron que al reducir el nivel NI en muestras MS de 15% a 10 y 5% decreció significativamente (&#945;= 0.05) en 21 y 31.5%, respectivamente, la sensibilidad de la prueba al virus BSMV. Similarmente, la reducción del número de muestras MS para formar muestras MC afectó significativamente (&#945;= 0.05) la sensibilidad de la prueba al virus e incrementó la probabilidad de registrar falsos negativos. Aunque el incremento y la reducción del TM de 10% a 15 y 5% afectó significativamente (&#945;= 0.05) la sensibilidad de la prueba al virus no se encontró una relación lineal entre el TM de la muestra MC con el valor de absorbancia. Este estudio evidencia la importancia del nivel de infección del virus BSMV en la semilla, del TM y del número de MS en muestras MC para la obtención de resultados confiables por la prueba DAS-ELISA.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract Detection of pathogens associated with seed requires to count with representative and homogeneous samples to obtain reliable results. This research objective was to evaluate whether: 1) the level of infection (5, 10 and 15%); 2) the increase in the number of single sample (10, 15, 20 and 25) used to form composite samples; and 3) increasing and reducing the usual sample size from 10% (established at CIMMYT for diagnosis of pests and diseases) to 15 and 5%, affect the predictive capability of DAS-ELISA test to barley stripe mosaic virus (BSMV). From seed lots of bread wheat (Triticum aestivum L.), one naturally infected and another healthy, 210 single samples (MS), 20 g each, divided into equal groups of 70 MS samples with 5, 10 and 15% level of infection (NI) to form sample sizes (TM) of 15, 10 and 5%, and composite samples (MC) with 10, 15, 20 and 25 MS samples by NI and TM were formed. The results indicated that reducing the level of NI in MS samples from 15% to 10 and 5% decreased significantly (&#945;= 0.05) in 21 and 31.5% respectively, the sensitivity of the test to BSMV virus. Similarly, reducing the number of MS samples to form MC samples significantly affected (&#945;= 0.05) the sensitivity of the test to the virus and increased the likelihood of false negative record. Although the increase and reduction of TM from 10% to 15 and 5% significantly affected (&#945;= 0.05) the sensitivity of the test to the virus, not finding a linear relationship between TM from the MC sample with the absorbance value. This study demonstrates the importance of the level of infection from the BSMV virus in the seed, of TM and MS number in MS in MC samples to obtain reliable results by DAS-ELISA.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[BSMV]]></kwd>
<kwd lng="es"><![CDATA[muestreo]]></kwd>
<kwd lng="es"><![CDATA[semilla]]></kwd>
<kwd lng="en"><![CDATA[BSMV]]></kwd>
<kwd lng="en"><![CDATA[sampling]]></kwd>
<kwd lng="en"><![CDATA[seed]]></kwd>
</kwd-group>
</article-meta>
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<given-names><![CDATA[V.]]></given-names>
</name>
<name>
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<given-names><![CDATA[P.]]></given-names>
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</person-group>
<article-title xml:lang=""><![CDATA[Distribution of Tilletia tritici and Microdochium nivale in wheat seed bulks, and significance for seed sampling strategies]]></article-title>
<source><![CDATA[Seed Testing International]]></source>
<year>2005</year>
<numero>129</numero>
<issue>129</issue>
</nlm-citation>
</ref>
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</back>
</article>
